ALCOHOL

POISONING
Dr Bilal Iqbal
ALCOHOL
 Ethanol
 Methanol
 Isopropanol
 Ethylene glycol
ETHANOL
Most commonly ingested alcohol.
ETHANOL SOURCES
 Alcoholic drinking
 Colognes
 Medical preparation
 Mouthwashes
 ANALYSIS OF ETHANOL
 Blood Ethanol  Breath Ethanol
 Enzymatic analysis :  Breath alcohol measurement devices use :

 Ethanol is measured by oxidation to  Infrared abrorption spectrometry

acetaldehyde with NAD a reaction is  Dichromatic sulfuric acid oxidation
catalyzed by ADH , NADH is measured at reduction (photometric)
340 nm that is proportional to Ethanol
present.  Electrochemical oxidation

 Serum or plasma is preferred over whole

blood to prevent interference from
hemoglobin.
ISOPROPANOL
(SECOND MOST COMMONLY INGESTED ALCOHOL)
Sources :
Industry solvent
Disinfectant
Skin and hair products
Jewelry cleaners
Detergents
Paint thinners
Anti freeze
ISOPROPANOL ANALYSIS
 Isopropanol and its metabolite Acetone determined by
 Headspace gas chromatography
 Nuclear magnetic resonance
METHANOL
 Sources:
 Solvent in commercial products
 Constituent of antifreeze and window cleaning fluids
 Componrnt of canned fuel