Screening of different Okra genotypes under Salt Stress

M. Hussnain Raza
Roll No: 2019-Gu-2388

Supervisor : Dr. Javeria Sherani

Department of Horticulture

Ghazi University ,Dera Ghazi Khan

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 Introduction

• Common Name Ladyfinger

• Botanical Name Abelmoschus esculentus

• Family Malvaceae

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 Conti…

• Okra (Abelmoschus esculentus L.) is recognized as annual

herbaceous plant grown in tropical and subtropical areas
• To the total production of 4.8 million ton pods of okra in the
world, India contributes 70%, Nigeria 15%, Pakistan 2%, Ghana
2%, Egypt 1.7% and Iraq 1.7%

• It was originated in India but now grown in many parts of world

including the Middle East, Africa, Brazil, Turkey ,Pakistan and USA

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 Conti…
•Okra serves as a source of carbohydrates, fats, vitamins and
various minerals

•Okra seeds containing good quality edible oil and high protein
are used to complement other protein sources

• Okra plant at prior growth stages is more susceptible to

salinity as it affects water relations and nutrient uptake of
plants.

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 Conti…
• During long term contact to salinity, plants familiarity ionic
stress, which can lead to early senescence of adult leaves
and thus reduction in photosynthetic rate is a common
observation

• Salinity creates water and ionic imbalance in plants due to

existence of poisonous ions. Plants affected by salt stress
show stunted growth and the leaf color gets darker

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 Conti…
• Many regions in the world contain soils which are too
saline for economic crops which affect the plant growth
through oxidative stress and osmotic effects

• About 10 billion dollars is the global annual loss in the

production of agriculture due to salt affected land

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 Adverse effects of Salinity on Okra

• Nutritional Status of the Plant

• Hormonal Level
• Juvenility
• Position of Plant Part

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 Objectives
The objective of this work was,

• To screen out the available okra cultivars for salt tolerance

• To analyze the morpho-physiological effects of salt stress
on okra

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Materials and Methods

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 Materials
• Different Genotypes of Okra (10)
• Sand
• Distilled water
• Pots

• NaCl Solution

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 Methodology
• The present Experiment conducted at College of Agriculture,
BZU Bahadur Sub Campus Layyah. The research work carried
out in an experiment. Experiment was laid out in completely
randomized design (CRD). The seeds of okra cultivars for the
study obtained from Ayub Agricultural Research Institute
(AARI), Faisalabad and NARC, Islamabad.

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 Methodology
• Screening of different okra genotypes under Salinity
 
Following cultivars will be included in the experiment

• OH-713
• OH-138
• OH-139
• OH-001
• OH-597
• MD-02
• 0H-152
• OKRA7100
• OKRA-7080
• SABZPARI
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 Methodology

Filling The Pots with Sand 14

 Methodology

Sowing of Okra seed 15

 Methodology

Diluting the Solution

NaCl Solution

Applying the different

treatments of NaCl

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 Materials and Methods
Site: Nursery Area, BZU Sub Campus Layyah
Plantation Time: May, 2021
Design: CRD
Treatments: 04
Replications: 03

• Different treatments of NaCl applied to Okra genotypes.

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 Treatments
Treatments Concentration

T0 Control (distilled water)

T1 NaCl 25 mM

T2 NaCl 50 mM

T3 NaCl 75 mM

T4 NaCl 100 mM

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 Parameters
• Emergence percentage (%)
• Germination percentage (%)
• Plant Height
• Shoot length (cm)
• Root length (cm)
• Shoot fresh weight (g)
• Shoot dry weight (g)
• Root fresh weight (g)
• Root Dry weight(g)

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 Statistical analysis
• The data collected for all experiments analyzed by using the
Fisher’s analysis of variance technique and LSD test at 5%
probability will be used to compare the treatment means.

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 Results and Conclusion
Means values of different parameters of Okra genotypes under different
treatments of salinity
Okra Genotypes G% E% PH RL SL RFW RDW SFW SDW Rank

OH-713 90.4 90.1 25.33 10.17 14.22 10.53 1.981 16.75 2.56 1

OH-138 85.6 84.2 20.11 8.66 10.90 09.21 1.777 14.88 3.64 3
OH-139 90.6 88.8 23.64 9.0 13.99 10.63 1.824 15.25 2.41 2
OH-152 80.2 80 18.31 8.47 10.88 9.99 1.626 14.63 3.00 4
OH-597 75.2 74.2 17.00 8.11 10.12 9.32 1.613 14.93 3.77 6

OH-001 70.4 70.2 14.33 788 9.91 8.66 0.987 1454 3.23 5

OKRA-7080 68.2 66.4 12.29 7.75 8.85 8.24 0.863 12.11 2.987 7

OKRA-7100 68.1 65.2 10.63 7.67 8 .56 7.63 0.789 11.50 2.621 8

SABZ PARI 65.8 64.2 9.65 7.11 9.38 7.41 0.701 11.74 2.52 9

MD-2 64.6 60.4 9.37 6.88 7.22 6.91 0.622 10.63 2.33 10

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 Conclusion
The germinated seedlings were compared according to different parameters i.e.

emergence % , germination percentage%, plant height , plant root fresh and dry Weight ,

shoot fresh and dry weight . On the basis of the Okra genotypes capacity of salt

tolerance and results, the okra genotypes OH- 713, OH-139, OH-138, and OH-001 were

among the most tolerant group and medium tolerant group included OH-152, OH-597,

and MD-02. Lower medium tolerant group was identified included the, Sabzpari, Okra-

7100 and Okra-7080. After the results of this our experiment. So, from the final results

the above categorized Okra genotypes are salt tolerance in saline soil and are good for

salt affected regions.

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