Mouse vibrissa follicles

Experiment

Purpose: isolate one hair follicle from vibrissae

General information: (A) Location of vibrissal follicles of the mystacial pad of the
mice. Vibrissal follicles are arranged in rows
(B) Schematic representation of the hair cycle phases of a
vibrissal follicle. Stage 1: mid-anagen; stage 2: late
anagen; stages 3–5: catagen; stage 6: early anagen;
stages 7 and 8: anagen. Mature and actively growing Hair
follicles anchored in the subcutis periodically regenerate
by spontaneously undergoing anagen (repetitive cycles of
growth), catagen (apoptosis-driven regression) and
telogen (relative quiescence), which is termed hair cycling
and is a typical characteristic of functional Hair follicles.
Experiment

Purpose: isolate one hair follicle from vibrissae

General information:

a: follicular papilla, b: capsula, c: dermal sheath, d:

ringwulst, e: lower vascular sinus, f: upper vascular sinus,
g: bulge region, h: matrix, i: hair, j: sebaceous gland. 

Mouse vibrissa follicles

Cultured in William's medium E 

Method

- Macrodissection of the whisker pad:

use tweezers to take a small part of vibrissae the use a scissor to cut it)
- Turn the whisker pad over, and from the dermal side to expose the ro
ws of vibrissae follicle bulbs. Cut between the follicle rows (use the knife
with clear cut)
- Isolate one hair follicle: use tweezers and knife to remove adipose tissu
e