+ Puromycin concentration: 2,3,4,5 µg/ml

(https://www.thermofisher.com/kr/ko/home/life-science/cell-culture/transfection/selection/puromycin.html?gclid=Cj0KCQiA2NaNBhDvARIsAEw55hiX65VeYWHR_kfQh3j2LlU--

AFYbJeLYOSvVgDDVTY3a41VwiUPHDAaArPzEALw_wcB&ef_id=Cj0KCQiA2NaNBhDvARIsAEw55hiX65VeYWHR_kfQh3j2LlU--

AFYbJeLYOSvVgDDVTY3a41VwiUPHDAaArPzEALw_wcB:G:s&s_kwcid=AL!3652!3!391186514773!p!!g!!

puromycin&cid=bid_clb_cce_r01_co_cp0000_pjt0000_bid00000_0se_gaw_nt_pur_con) -> This web recommend Adherent mammalian cells are sensitive to

concentrations of 2 to 5 µg/ml (But in our experiment Puromycin: 1 µg/ml works in iDPC)

+ .org/10.1371/journal.pone.0079434) -> This paper used puromycin (2 µg/mL; Sigma) for 4 days
 My experiment in 48 well plates

Puromycin 0 µg/ml 104 2x104 3x104

Puromycin 1 µg/ml 104 2x104 3x104

Puromycin 2 µg/ml 104 2x104 3x104

Puromycin 3 µg/ml 104 2x104 3x104

104 2x104 3x104
Puromycin 4 µg/ml
Puromycin 5 µg/ml 104 2x104 3x104

Cell confluency 20% 50% 80%

-> Find the Cell confluency and the lowest concentration puromycin that kills 100% of cells in 2-3 days