Activity no.

9
Respiratory
SERABANI, RAMOS, TAPIA, TATEL & ZARANDONA
[BS BIO 3C - GROUP 5]
 For hands On:  You will have a bonus points for the conduct of the study

Objectives:

• Gain experience doing simple chemical assay

• Learn of differences in biochemical composition of tissue

• Learn importance of respiration in living tissue

Materials:
• Bean, Corn, and peanut seeds

• Clear glass jars of varying volumes with airtight lids

• Graduated cylinders

• Metric rulers

• Single edged razor blades

• Paper towels

• Dark shelf or cabinet area to store respiration chambers

RAMOS
Introduction:

In this activity the students were asked to perform the respiration activity, using
the following materials: Beans, Jar containers that comes in different sizes,
Graduated cylinder or beaker, Paper towel or Tissue paper, Water, Metric Rulers and
etc. Particularly, the students is said to measure the respiration of the germinated
bean seed inside the jar container.

The experiment is expected to last for 5 days to obtain the final measurement
of the average shoot growth, allowing the us the students to compare results from
different set-ups, and be able to understand the difference between the data of one
group to another.

The germinating seeds such as the monggo seeds were tasked to utilized in
this activity for it is because it can clearly emphasize the concepts behind the
activity.
 TATEL
Bring in glass jars. The best jars clear and have wide mouth. There should be a
set of five or six jars of decreasing volumes for each class period. The largest
can have a volume of approximately one liter and the smallest a volume of
about 100 ml.

A range of volumes should be used in each class. Per student or depending on

the management of the group / should determine the volume of their jar by
filling them with water measuring the water volume. The initial volume of
oxygen in the jar is calculated by knowing air is 21% oxygen. (Hints: oxygen:
air=0.21 and 1mL=1 cm.

After four or five days, the students can remove the jars from the dark location
long enough to estimate shoot length (the lid must remain tightly closed so that
more oxygen will be introduced). The jars will then be replaced in the dark
cabinet.

In another three or four days, the jars may be opened and the shoot lengths
measured. Student now have enough data to construct a graph plotting average
length of shoot against oxygen volume. Estimated shoot length on (date), make
final measure of lengths on (date).
 Data and Observations:
Average Shoot TAPIA
Length
Jar OxygenVolume Date(Estimated) Date(Final Comments
Measure)
I (200 mL) x 0.21= 5 days 2.4 cm Jar I, obtained a total average shoot length of 2.4calculated by means
42 of getting the total length of each bean that grows inside the jar for 5
days, and adding the 12 sample (length), divided by its original
number allowing us to record the final measurement. When
comparing the recorded data to the rest, it is obviously higher than Jar
I & II but lower than Jar IV and V.

II (100 mL) x 0.21 5 days 1.9 cm Jar II, with 100 mL volume of water, having 21 oxygen volume
= 21 accumulated a final growth measure of 1.9 cm, the second lowest
recorded growth out of all treatment.
III (50 mL) x 0.21= 5 days 1.5 cm Jar III, with a jar volume of 50 ml when tabulated to know its oxygen
10.5 volume resulted to 10.5. Due to its size that is the smallest jar out of
the 5 set-ups, it’s also expected to record the lowest final
measurement with 1.5 cm.
lV (250 mL) x 0.21= 5 days 3.6 cm Jar IV, with 250 mL and has an equivalent oxygen volume of 52.5,
52.5 after 5 days recorder a mean value of bean growth with 3.6 cm, quite
lower than Jar V, but higher that Jar I, II & III.
V (300 mL) x 0.21= 5 days 4.2 cm Jar V, having oxygen volume of 63 recorded a final average shoot
63 length of 4.2 cm that when compared to other set-ups is higher that is
due to the size of the jar container.
SERABANI

Observations:
The results of the activity showed that, Jar V (300 mL) received the highest number of
average shoot growth with 4.2 cm, followed by Jar IV (250 mL) with an average shoot
growth of 3.6 cm, next is Jar I (200 ml) with 2.4 cm average shoot growth, the second to
the last is the Jar II (100 mL), with 1.9 cm. Lastly, the set-ups with lowest average shoot
growth is the Jar II with only 1.5 cm. The results only foresee that the larger the container,
the higher the oxygen volume resulting to higher average shoot growth.
Students should construct a line graph using the data from all groups. That is, average shoot length should be plotted against oxygen volume.
ZARANDONA

Generalization:
In this activity we were able to learned that germinating seeds like Monggo seeds
(Green Dry Beans) are the best representation for the respiration experiment. For such
genuine reason, is that germinating seeds can respire actively allowing them to germinate
faster by means with the presence of water required for their growth, as well as the oxygen.
As the seeds actively respire, what as observed is that they are also taking in oxygen, in
which a large portion of oxygen absorbed by the germinating seeds is base on the length of
the germinated beans. We also learned that, the bigger the jar container used in the respiration
experiment the larger the average shoot growth.