RECOVERY &

PURIFICATION
• Mycelium is separated from broth by filteration & streptomycin is recovered.
• Recovery process – broth is acidified, filtered & neutralized. Then its
subjected to column containing cation exchange resin to adsorb
Streptomycin from the broth & column iswith water & streptomycin eluted
with HCl before concentration in vacuo almost to dryness.
• The streptomycin is dissolved in methanol & filtered.
• Acetone is used in filterate to precipitate the antibiotic.
• Precipitate is washed with acetone & dried in vacuo.
• Purification is done by dissolving in methanol to form pure S. chloride
complex. Further by, adsorbing it onto activated charcoal& eluting with acid
alcohol
 Final operations

• It includes the following steps like:

• Then, dissolve the crystalline streptomycin in the solution containing pyrogen-free water.Pass the solution through the special
biological filter like Seitz filter to eliminate the microorganisms that can contaminate the streptomycin
• Then, dry the filtrate under vacuum to form a dried cake.Crush the dried cake into a powdered form by employing high vacuum
sublimation using sterile steel balls.
• After that, weigh the powdered streptomycin into sterile vials.
• The whole process needs to be carried out in the air-conditioned room, which provides filtered or stale air through the
ultraviolet lamps
• .Finally, package the powdered streptomycin in the rooms having 10% relative humidity
• .
Uses of Streptomycin
• Treatment of diseases
• Tuberculosis
• Plague
• Veterinary medicine against gram negative bacteria
• Pesticide & fungicide
• Cell culture.