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Lab.7
INDOLE ALKALOIDS
INTRODUCTION
Indole alkaloids are a class of alkaloids containing a structural
moiety of indole.
Indole is an aromatic heterocyclic organic compound. It has a
bicyclic structure
INTRODUCTION
HARMALA ALKALOIDS:-
A. Harmane
B. Harmine
C. Harmaline
D. Harmalol
E. Tetrahydroharmine
F. Vasicine & Vasicinone (quinazoline derivatives)
ISOLATION OF THE HARMALA ALKALOIDS
Aim: - To isolation the harmala alkaloids
Equipments and reagents:-
1) Large beaker 9) 2%HCL
2) Reflux instrument 10) Ammonium hydroxide solution
3) Water bath 11) Chloroform
4) Large evaporating dish 12) Methanol
5) Separatory funnel
6) Litmus paper
7) Petroleum ether
8) 90 % ethanol
Procedure:-
1) Take 50 gm. of harmala seeds.
2) Macerate with 500ml of petroleum ether over night.
3) Filter off the maceration solvent of the seeds.
4) And then reflux with 500ml of 90 %ethanol for one hour.
5) Evaporate the resultant filtrate on the steam bath to syrup liquid.
6) Add to the concentrated filtrate 5 ml of 2%HCL.
7) Remove all the remaining alcohol by evaporating in large evaporating dish on
the steam bath.
8) The acidic extract is then cooled, filtered and placed in a separatory funnel where
it is made alkaline by adding ammonium hydroxide solution (according to litmus
paper).
9) The alkaline solution is then extracted three times with 100 ml of chloroform.
10) The chloroform solution is then concentrated to give the product crude alkaloid
(fraction A).
11) Add 10 ml methanol to fraction A, then filter, the result is two fractions,
fraction B (filtrate), fraction C (precipitate).
Results:-
Fraction A contains the crude alkaloids.
Fraction B contains (vasicine and deoxyvasicine alkaloids)
Fraction C contains (harmine and harmaline).
Discussion:-
1) Maceration process is for defatting process.
2) Use of reflux condenser since the plant is not affected by heat.
3) Use of methanol is to separate some of harmala alkaloids, since some are soluble
in methanol and others are not.
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