Bacterial Transformations

7007BMS
 Basic Health and Safety
• No eating/drinking/chewing gum
• No mobile phones, should be left in your locker
• Long hair must be tied back
• Lab coats must be done up and safety specs worn as
appropriate
• When you have finished your work ensure you tidy
your bench
• Remove your lab coat before you wash your hands
 Safety

• Flammable liquids + Flames

• Be aware of what you are doing

• Be aware of where your limbs are !!!
 Streaking vs Spreading Plates
• Demo (Be as sterile as you can)
7007BMS Lab report
 Step 2 Transformation

Molecular
Biology
GLP1R + GLP1R Transformation
Site Directed
Mutagenesis X

PCR
 Transformations
Insert; gene of interest eg huGLP1R ; mutated/WT

Transformation
Introduction Amplification of
into host cell recombinant DNA
by batch growth of
Bacteria/yeast/insect cells bacteria

Selection
for cells containing
Marker: recombinant DNA
Antibiotic resistance molecules by
Gene growth
Ampicillin in the presence of
Kanamycin antibiotic
 End the practical
The real deal 

Mutant number, initial, date

Control, initials, date