RELATIONSHIP BETWEEN WATER TEMPERATURE AND

LATENCY PERIOD OF CLARIAS GARIPINUS INDUCED USING

OVAPRIM

BY

NDEN NANFA
17/42744/UE

COLLEGE OF FORESTRY AND FISHERIES

DEPARTMENT OF FISHERIES AND AQUACULTURE

DECEMBER, 2022.
 INTRODUCTION
 Peak production of eggs by the female and hatching of the
fertilized eggs are known to take place during the rainy season.
The widespread use of induced breeding technique has permitted
the breeding of a number of fish species that do not ordinarily
spawn under confinement.
 Latency period is the time interval from the first injection
to ovulation. The ‘‘latency period’’ varies greatly among
species (Agbebi, et al., 2013).
 Due to limited information on latency period in relation
with temperature the researchers has decided to
investigate the relationship between water temperature
and latency period of Clarias gariepinus induced using
Ovaprim.
 JUSTIFICATION
• The demand for fish as food, recreation, and
ornamental aquariums is steadily increasing, however
there is a decline in Natural fish populations produced
due to pressure on fish in natural bodies as well as
environmental degradation and over-fishing.
• This has resulted in an increased effort in the
development of techniques for hatchery production of
fish. However there is paucity of information about the
period, time and temperature of induced ovulation of
Clarias gariepinus this has made it difficult for the
smooth induced spawning, making it necessary to
carry out this study inorder, to fill this gap thereby
increasing the number of Clarias gariepinus produced.
 AIM OF THE STUDY
The main objective of the study is to examine the relationship between water
temperature and Latency period of Clarias gariepinus induced using Ovaprim.

OBJECTIVES OF THE STUDY

1. Determine optimal temperature for
ovulation of eggs in Clarias gariepinus
2. Determine the best latency period for
ovulation of eggs in Clarias gariepinus
 MATERIALS AND METHODS
Experimental Site/Fish
• The experiment will be performed at the Department of
Fisheries and Aquaculture Laboratory where 10 broodstock
of Clarias gariepinus will be collected from, JOSTUM Fish
farm and taken to the control laboratory in well aerated
plastic containers. Ten 14-months-old Female brood-
stocks weighing 300 g each of Clarias gariepinus will be
acclimatized for one week.
• The temperatures for the experiment will be
24,26,28,29,30 ᵒC, and the ten broodstock will be
distributed into 5 bowl, in a ratio of 2 fish per bowl.
• The Latency period will be determined for each
temperature and weight of eggs ovulated at each
temperature.
 MATARIA AND METHODS CONTINUE.
 1 g of eggs = 600 eggs, therefore, 20 g of eggs = 12,000 eggs
Wet fertilization method will be used. The testes from the male
fish will be removed and milt pressed into a sterile dry petri dish
and diluted with physiological solution prepared by diluting 9 g of
common salt in 1 L of water.
The milt suspension will be drawn to fertilized the eggs to be
stripped. After 2 min of gentle stirring with plastic spoon, the
fertilized eggs will be washed several times with fresh water to
remove excess milt. The fertilized eggs will be spread on a nylon
mesh hatching substrate called kakaban in an incubation tank with
a regulated flow of water until hatching occurs.
• The weight of fish before injection will be taken and after
stripping will be taken
The percentage fertilization will be estimated as;
• Fertilization (%) =
• Counting of hatched larvae:
• The hatched larvae will be counted while the un-
hatched eggs will be discarded; the percentage
hatchability will be estimated thus:
• Hatchability (%) =
• Analysis of data: Data will be analyzed using one-
way analysis of variance in a completely
randomized design. Significant means will be
separated using Duncan Multiple range test.