COURSE-BIOCHEMICAL ENGINEERING

COURSE CODE-19CH7DCBCE
SEMESTER-7’TH
COURSE COORDINATOR-PROF. MAHESH SHETTY
PRESENTATION ON-PRODUCTION OF PENICILLIN
PRESENTED BY-SHWETA GARG (1DS19CH034)
 1.INTRODUCTION

2.HISTORY

CONTENTS
3.CONDITION FOR
PRODUCTION OF
PENICILLIN

4.PRODUCTION METHOD
 Introduction

WHAT IS PENICILLIN ?

• Penicillin is an antibiotic.
• It is used to treat infections caused by
bacteria.
• It does not work on viral infections (such as
colds and flu).

3
 It was accidently discovered and
massively used in world war 2nd to the
nobel prize for medicine in 1945.

Pencillin has made its proof as the

MIRACLE DRUG as that
revolutionized the course of medical
industry.

HISTORY
CONDITION FOR PRODUCTION OF
PENICILLIN

Penicillin is an aerobic organism; oxygen supply is critical: reactor must

have an efficient oxygen supply system.
• The optimum pH for penicillin growth is 6.5: maintain pH efficiently (pH
controller and acid-base reservoir).
• Strain Stability problems (mutations): careful strain maintenance is re-
quired.
• Biomass doubling is about 6h: provisions must be made.
Production Method
• Fermentation is the technique used for the commercial production of penicillin.
• It is a fed-batch process that is carried out aseptically in stainless steel tank reactors
with a capacity of 30 to 100 thousand gallons.
• The whole process is aerobic (it requires air). The fermentation is carried out over 4 or 5
days at 25-27°C. This gives a yield of about 0.5% penicillin.
• Penicillin rich aqueous broth was treated with activated charcoal to remove pigments
and impurities. After filtration and carbon treatment, penicillin recovery was done by
liquid-liquid extraction (solvent extraction). Penicillin was extracted from an aqueous
phase into the solvent butyl acetate.
• The penicillins are the most commonly used antibiotics in pediatrics and can be divided
broadly into four different groups: (1) natural penicillins, (2) penicillinase-stable
penicillins, (3) aminopenicillins, and (4) extended-spectrum penicillins
 USAGE OF PENICILLIN

Used as drug, to treat infections caused by bacteria

Penicillins are available only with your doctor's prescription.

This product is available in the following dosage forms:

Powder for Solution
Tablet
Solution
thank you