PROSPECTION OF MONO AND DUAL-SPECIES Staphylococcus aureus

and Escherichia coli BIOFILM FORMATION IN STAINLESS STEEL

MORAES, J. O. ; CRUZ, E. A. ; VENTURA, R. L. ; MELO, A. P. F. S. ; UNGARETTI, J.

a a b a

S. ; SOUZA, E. G. F. MORAES, M. O. BARBOSA, L. A.

a c d e

E-mail: ju.omoraes@yahoo.com.br
Department of Agroindustry, Federal Institute of Alagoas, Piranhas, State of Alagoas, Brazil.
1

2
Department of Agronomic Engineering, Federal Institute of Alagoas, Piranhas, State of Alagoas, Brazil
3
Department of Agroecology, Federal Institute of Alagoas, Piranhas, State of Alagoas, Brazil
4
School of Nursing and Pharmacy, Federal University of Alagoas - University campus of Maceió
5
Faculty of Nutrition, Federal University of Sergipe -University campus of Lagarto, Brazil
TABLE 1 - Mean values of the number of viable sessile cells of strains isolated
INTRODUCTION
from Staphylococcus aureus in monospecies, submitted to two incubation
temperatures and classification for biofilm formation.
Among bacteria It is more common that they live by sticking to surfaces and
Incubation temperatures
forming organized communities called biofilms. In nature, biofilms can be composed Strains 8 °C 35 °C
of a single species or represent a consortium of numerous species. When present in   Log UFC/cm² Biofilma Log UFC/cm² Biofilm
N16 4,8 Non-Forming 6,8 Forming
the form of multispecies biofilms appear to be more resistant to antimicrobial activity N9 5,8 Forming 6,8 Forming
than to monospecies form (BRIDIER et al., 2015). B11 5,0 Forming 6,1 Forming
B41 4,8 Non-Forming 5,2 Forming
Among the bacteria that form biofilms on surfaces used in the food industries
N19 4,8 Non-Forming 5,5 Forming
responsible for foodborne diseases are Staphylococcus aureus and Escherichia coli. N29 5,0 Forming 6,8 Forming
Restricting the growth and development of these bacteria is very important, since B12 4,5 Non-Forming 6,6 Forming
6POD 4,9 Non-Forming 5,8 Forming
these microorganisms have a facility to form biofilms on various surfaces, making its
N28 5,0 Forming 6,5 Forming
eradication not always successful (ORAL et al, 2010) . N26 4,9 Non-Forming 6,8 Forming
N7 5,2 Forming 6,7 Forming
OBJECTIVE N30 4,6 Non-Forming 5,3 Forming
N21 4,9 Non-Forming 6,2 Forming
The objective of this study was to evaluate the capacity of Staphylococcus N11 4,6 Non-Forming 6,1 Forming
N20 4,8 Non-Forming 6,3 Forming
aureus biofilm formation and the influence of the presence of Escherichia coli on the
S62 4,7 Non-Forming 6,1 Forming
formation of stainless steel duospecies at 8 ° C and 35 ° C.
TABLE 2 - Mean values of viable sessile cell numbers of isolates of
MATERIALS AND METHODS Staphylococcus aureus and Escherichia coli ATCC 25922 dualespecies, submitted
to two incubation temperatures and classification for biofilm formation in BP culture
medium.
Isolation and identification of strains of Staphylococcus aureus
isolated at milk collection points Incubation temperatures
Strains 8 °C 35 °C
  Log UFC/cm² Biofilma Log UFC/cm² Biofilm
Standardization of bacterial cells (VALERIANO et al., 2012) B11 dualspecie 4,4 Non-Forming 6,3 Forming
N9 dualspecie 4,4 Non-Forming 6,4 Forming
N29 dualspecie 4,8 Non-Forming 6,2 Forming
Cleaning and sanitizing of stainless steel test surfaces
(MEIRA et al., 2012) N28 dualspecie 4,1 Non-Forming 6,6 Forming
N7 dualspecie 4,6 Non-Forming 6,3 Forming
a
Classification regarding the biofilm production expressed following the interpretation as
Evaluation of adhesion and biofilm formation on stainless steel surfaces described by Mallezi et al. (2012).
(MILLEZI et al., 2012)
CONCLUSION
RESULTS It has been observed that the presence of E. coli may influence the biofilm
formation capacity of S. aureus, and it is evident the formation of multispecies
A total of 132 strains were isolated, however only 12.12% (n = 16) strains
biofilms of E. coli and S. aureus in stainless steel under the temperature conditions
were biochemically confirmed as S. aureus. It was observed that only 31.25% (n =
evaluated.
5/16) of the analyzed strains were able to form monospecies biofilm at 8 °C while at
ACKNOWLEDGEMENTS
35 °C all strains were biofilm-forming (Table 1).
To Instituto Federal de Alagoas through support provided for project execution.
Five strains of S. aureus (n = 5/16) were selected that showed the capacity of
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