Ali Raza

19014107-034
Applications of GC in Blood
Analysis
Determination of amphetamine-type stimulants in blood and
urine

 Famous amphetamine-type energizers

 Serious cultural issue
 A technique in light of gas chromatography-pair mass spectrometry (GC-MS/MS)
with basic and quick fluid extraction (LLE) and derivatization was created and
approved for the synchronous assurance of the six previously mentioned
amphetamine subsidiaries in blood and pee.
Cond..

 Extraction method
 Approval boundaries
 Scientific toxicological cases
 High selectivity
 Non-lethal inebriation cases in routine toxicology examination
Blood Analysis by Headspace Gas
Chromatography
 Imperial Courtrooms
 Overabundance measure of sodium fluoride (NaF) additive, which would build
the grouping, not entirely settled by headspace gas chromatography (HS-GC).
 The indictment didn't deliver master proof to invalidate this contention and the
alcoholic driving suspect was absolved.
Cond..

 HS-GC examination
 The utilization of a proportion likewise assists with making up for any salting-out
impact of ethanol.
 the grouping of ethanol was brought down significantly more when a 4-carbon
liquor (t-butanol) was utilized as the inner norm.
Rapid quantitation of cyanide in whole blood
by automated headspace gas chromatography
 Cyanide (CN), a compound asphyxiate, is a quickly acting and strong toxic
substance.
 the utilization of gas chromatography (GC) with nitrogen-phosphorus
identification and acetonitrile as an inner reference
 fast conclusion of CN in clinical and legal examples
Study of silylated derivatives in blood by gas
chromatography
 Synchronous semi quantitative/quantitative screening of 51 medications of
misuse or medications possibly unsafe for traffic security in serum, plasma or
entire blood.
 High return fluid extraction (LLE)
 Restriction of quantitation (LOQ)
Quantitation and identification of ethanol and inhalant
compounds in whole blood using static headspace gas
chromatography
 Gas chromatography (GC) and vacuum bright spectroscopy (VUV)
 fire ionization recognition (FID)
 quantitative assurance of ethanol in blood and ID of inhalants
 Nonaromatic VOCs
 References: (APA)

 Woźniak, M. K., Wiergowski, M., Aszyk, J., Kubica, P., Namieśnik, J., & Biziuk, M. (2018). Application of
gas chromatography–tandem mass spectrometry for the determination of amphetamine-type stimulants in
blood and urine. Journal of Pharmaceutical and Biomedical Analysis, 148, 58-64.
 Jones, A. W., & Fransson, M. (2003). Blood Analysis by Headspace Gas Chromatography: Does a deficient
sample volume distort ethanol concentration?. Medicine, science and the law, 43(3), 241-247.
 Calafat, A. M., & Stanfill, S. B. (2002). Rapid quantitation of cyanide in whole blood by automated
headspace gas chromatography. Journal of chromatography B, 772(1), 131-137.
 Gunnar, T., Mykkänen, S., Ariniemi, K., & Lillsunde, P. (2004). Validated semiquantitative/quantitative
screening of 51 drugs in whole blood as silylated derivatives by gas chromatography-selected ion monitoring
mass spectrometry and gas chromatography electron capture detection. Journal of Chromatography
B, 806(2), 205-219.
 Diekmann III, J. A., Cochran, J., Hodgson, J. A., & Smuts, J. (2020). Quantitation and identification of
ethanol and inhalant compounds in whole blood using static headspace gas chromatography vacuum
ultraviolet spectroscopy. Journal of Chromatography A, 1611, 460607.
THANK YOU