A ASMAWATI S

ANTIOXIDANT ACTIVITY
TEST OF TELANG FLOWER
ETHANOL EXTRACT
(CLITORIA TERNATEA L.)
FROM PANGKEP DISTRICT
WITH DPPH METHOD
 INTRODUCTION

Many plants contain flavonoids, one of which is telang flowers. Telang flower
(Clitoria ternatea L.) is a plant whose all parts have benefits for humans (Marpaung,
2020). Telang flowers (Clitoria ternatea L.) contain flavonoids, glycoside flavonols,
anthocyanins, glycoside quercetin, glycoside kaempferol, glycoside mirisetin
(Kazuma, et al. , 2013), In addition, telang flowers also contain tannins, terpenoids
and steroids (Gupta, et al., 2010).
Telang flowers are commonly found in Indonesia, one of which is in Pangkep
Regency and the people have become accustomed to using the plant as traditional
medicine. According to Marpaung (2020) the benefits of telang flower extract are as
TELANG FLOWERS an antioxidant, anti-inflammatory, anti-microorganism, anticancer.
(CLITORIA TERNATEA L.)
Telang flowers
Extract water
contain anthocyanins.
parts of flowers,
Anthocyanins are
leaves, and roots Telang flower
also believed to be A good solvent A good solvent
of Telang flowers (Clitoria ternatea
antioxidants. The used to measure used to measure
have the potential L) which grows in
method used is antioxidant levels antioxidant
as a source of West Denpasar has
maceration or rated IC50 found levels rated IC50
antioxidants, a strong category
dissolving telang in telang flowers found in telang
especially the antioxidant activity
flowers in water. (Clitoria ternatea flowers (Clitoria
parts of flowers with an IC50
Extraction of telang L.) by using DPPH ternatea L.) by
that are extracted value of 87.86
flowers can be done is a methanol using DPPH is a
by boiling ppm
using water solvents solvent methanol solvent
treatment.
or aquades heated to a
 
temperature of 100
°C.

Purwaniati et Ukhradiya Setia Apriani, Cahyaningsih,

Erna, et al, 2019
al., 2020 et al, 2022. 2020. et al, 2019

So it is necessary to conduct a study on the activity test of ethanol extract of

telang flowers (Clitoria ternatea L.) from Kab. Pangkep with DPPH method.
 RESEARCH METHODE

This type of research is an experimental study by

analyzing antioxidant activity where telang flower
ethanol extract (Clitoria ternatea L.) is able to ward off
DPPH reagents which are free radicals.

Type Of Research Time and Place

It has been held from February to June 2022 at Makassar

City Health Laboratory Center
 RESEARCH METHODE

Working Scheme

Sample processing Extraction Manufacture of DPPH reagent solution

Manufacture of stock solution of telang Determination of maximum wavelength (λ

flower ethanol extract max)

Determination of IC50 ethanol extract of

Manufacture of vitamin C stock solution
telang flowers

Data Analysis
 Research Results

Table 4.1 Ic 50
calculation results from telang flower ethanol extract
Consensation of telang % Inhibisi Linear Line Equation
flower ethanol extract Ic50
(ppm)

10 8,34 y= 0,6394x + 2,58 74,22

20 15,64
=0,99958
30 22,56
40 28,56
50 33,81
 Research Results

Table 4.1 Ic 50
calculation results from Vitamin C
Consensation of telang % Inhibisi Linear Line Equation
flower ethanol extract Ic50
(ppm)

10 11,08
20 19,34
y = 61.167x - 0.4955
30 22,56 0,82
R2 = 0,99617
40 30,81
50 48,30
Grafik Hubungan Antara Konsentrasi Ekstrak Etanol Bunga
Telang Dengan % Inhibisi DPPH

Ekstrak Etanol Bunga Telang

40

35
f(x) = 0.6386 x + 2.624
30 R² = 0.995554729662959

25

20

15

10

0
5 10 15 20 25 30 35 40 45 50 55
Grafik Hubungan Antara Konsentrasi Larutan Pembanding Vitamin C
Dengan % Inhibisi DPPH

Vitamin C
60

50
f(x) = 61.1669811320755 x − 0.495471698113207
R² = 0.993522413346413
40

30

20

10

0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
 CONCLUSION

Based on the results of research that has been carried out, it can be
concluded that the ethanol extract of telang flowers (Clitoria ternatea L.) from
Pangkep Regency using the DPPH method has a strong category antioxidant
activity with an IC value of50 74.22 ppm.
 

TELANG FLOWERS
(CLITORIA TERNATEA L.)
 REFERENCES
 Purwaniati et al., 2020. Analisis Kadar Antosianin Total Pada Sediaan Bunga Telang (Clitoria
Ternatea) Dengan Metode Ph Diferensial Menggunakan Spektrofotometri Visible. Jurnal
Farmagazine Vol. VII No.1. Hal 18-25
 Ukhradiya et al, 2022. Antioxidant Activity of Telang (Clitoria ternatea L.) Extract in
 Inhibiting Lipid Peroxidation. Current Biochemistry 9 (1) : 26-37.
 Setia Apriani, 2020. Uji Aktivitas Antioksidan Ekstrak Bunga Telang (Clitoria yernatea L.)
dengan Metode DPPH. Fakultas Kedokteran Universitas Muhammadiyah Medan.
 Cahyaningsih, et al, 2019. Skrining Fitokimia dan Uji Aktivitas Antioksidan Ekstrak Etanol
Bungha Telang (Clitoria ternatea L.) Dengan Metode Spektrofotometri Uv-Vis. Jurnal Ilmiah
Medicamento Vol 5 N0.1
 Erna, et al, 2019. Krining Fitokimia Dan Uji Aktivitas Antioksidan Ekstrak Etanol Bunga
Telang (Clitoria Ternatea L.) Dengan Metode Spektrofotometri Uv-vis. Journal Ilmiah
Medicamento Vol. 5 No.1. Hal. 51-57.
 Makasana, J., & Dholakiya, B. Z. (2017). Extractive determination of bioactive flavonoids
from butterfly pea (Clitoria ternatea Linn.). Research on Chemical Intermediates, 43(2), 783–
799. https://doi.org/10.1007/s11164-016- 2664-y
 Kazuma, K., Noda, K., and Suzuki, M., (2013). Flavonoid composition related to petal color in
different lines of Clitoria ternatea. Phytochemistry, 64 (1133-1139).
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