Bioreactor

Team #21

Team Leader: Aigerim Tuishieva

Associate Leader:Aray-Bibi Serikbay
Aslan Nauyryzbay
Yernar Amanbay
Merey Zhumayeva
Azamat Toktarov
Aigerim Abdirassyl
Nuraiym Paiyz
01 Contents
Stirrer design and modelling

02 Contents
Bioreactor

03 Contents
System electronics

04 Contents
Measurements (Benchmark Experiment, Production
Phase)
Name of the member Contribution to the project

Aslan Nauyryzbay 3D Modelling and Printing

Aigerim Tuishieva

Ernar Amanbay Arduino Modelling and Coding

Merey Zhumayeva

Azamat Toktarov
Yeast Production
Aigerim Abdirassyl Report + Video/Image material
Test of the bioreactor
Aray-Bibi Serikbay Report + Video/Image material
Test of the bioreactor

Nuraiym Paiyz
 The control process
Design Arduino Benchmark Yeast
Modelling Experiment Growth
Process

1 2 3 4 5 6 7 8 9 10

CAD Design of 3D Connecting DC Motors

Modelling Arduino UNO for stirrer Turbidity Pumping process pH Level Preparation of Collection of Yeast
Modelling Bioreactor values
and sensors and Pump Medium
parts
 Schematic design of
bioreactor
Stirrer design & modelling
 
 Our design of bioreactor
DC Motor
Hydrofoil Holder
impeller

pH Sensor and
Float Switch
Holder

Turbidity
Sensor
 Some minor issues
during 3D Printing
The lack of instruments
1 The metal shaft had to be cut manually by
using pliers

Time
2 The process took a significant portion of
assembly time
 A RDUINO
CODING
PROCESSING
&

❑We connected almost every sensor and board

through breadboard. Moreover, we connected 5V
and ground of Arduino UNO to + and – lines.
❑Before fixing pH probe into the plastic jar, we
collaborated sensor using two solutions provided
in laboratories ( pH solution of 4.0 and 7.0). We
have got coefficients in order to convert voltage
measured by pH sensor into actual value that we
further used in coding.
❑We connected two DC motors with ARDUINO
UNO through L298N driver board.
 ARDUINO CODING

Code for pH Sensor

Code for DC motors for stirrer and pump

Code for Turbidity Sensor
1. Code checks whether switch closed or opened.
2. If it is closed, it checks pH of the medium. If it is opened, pump and stirrer
stop.
3. If pH<4, water pump starts to work to increase the pH. If pH>4, stirrer
starts to work instead of water pump.
 The main function

Fresh Farm
ORGANIC food Fresh
 Difficulties during
coding and assembling
Changing the type of the board connecting pH
Solution
sensor with Arduino UNO. Collaborated pH
sensor two times.

The pH probe in the jar suddenly break down and

did not record pH values.

Absence of power supply needed to run DC

motors.

Charger of the phone(12V of L298N)

 Calibration of turbidity sensor
It is carried to assign voltages to the amount of turbidity in the solution in the tank. For this purpose, we used different masses of yeast and added little amount
of yeast every time to the medium 129+previous yeast.  We obtained the curve of pH during whole experiment and it showed inverse proportionality between
the amount of yeast added and the ph. The range at which pH was changing is 0-5, e.g. when the capacity of yeast in the container was 10 grams, we obtained
the lowest pH available.
 Testing Phase
(Benchmark Experiment)
Peristaltic pump and pH sensor
We poured 1lt of water in our container
and started stirring. Then, gradually
added vinegar to that container with
water and measured the pH. Vinegar
was added til the peristaltic pump
automatically begins pumping.
Systems’ stirring capability
We started the stirrer motor and mixed 10
drops of food color with 1lt of water. This
process stopped after 1.5mins and turbidity
values curve was presented over the duration
of this test.
pH-values curve
We presented the pH-values curve over the
duration of the process
04
A sustainable liquid flow
03
We started the peristaltic pump and
demonstrated the sustainable liquid flow
in the main container till the liquid level
02 sensor is activated and automatically
stops the pumping process
01
 Results of Benchmark Experiment

Content Here

Content Here

Content Here
 Difficulties during
benchmark experiment

Simultaneous Required
operation of power supply
turbidity and pH of peristaltic
sensor pump was high

Monitoring the
values of pH
 The process of yeast production
1 2

1 We have pilled 200g potato and then sliced

it.

01 02 2
Sliced potatoes were boiled in 1 liter water
Text Here Text Here for 1 hour.
You can simply You can simply
impress your impress your
audience and add audience and add
a unique zing a unique zing

04 3 It was filtered by the use of cheesecloth.

Text Here
You can simply
impress your audience
20g dextrose was added before cooling of
and add a unique zing
4 the solution with starch.

3 4
 The
Yeast Growth
✔ 1.5 liter of prepared medium +
1,5 g of dry yeast
✔ T = 31ºC (as optimum
temperature for yeast is 30-
35ºC

✔ Aqueous solution NaHCO3

was used for pH regulation
✔ t (time) ≈ 10 hours
✔ pH levels measured every 3
seconds.
 Results
Color of Medium
It has changed from pale green
to yellowish.

Smell
Like flour and bread

pH
Were between 5-6
Results

MAIN
TITTLE
Collection Of Yeast

1 Step

Four samples from obtained yeast was

filtered through a coffee filter. They are
separately poured into empty glass jars.
2 Step 3 Step

We revealed carefully the conical In order to dry the wet yeast, the filters
coffee filter paper (solid obtained in were put in the oven at 50ºC for 2
the coffee filter contains yeast). hours.
 4 Step
Results
Dry yeast was collected from each
filter and weighted for further
calculations. Table 1. Table 2.
The output of yeast growth Results of yeast growth process

Yeast input (g) 1.5 Inoculum Final Total Duration Duration Duration Overall Overall Dextrose
conc. conc. fermentation       cellular volumetric conc. %
Yeast output from filter 1 (g) 0.82 (g/l) (g/l) time(h) of step 1 of step 2 of step 3   Productivity v/v
Yeast output from filter 2 (g) 0.67 (h) (h) (h) yield at the end
(g) (g/l/h)
Yeast output from filter 3 (g) 0.90
Yeast output from filter 4 (g) 0.72 1.0 2.07 10 3.1 2.5 4.4 3.11 0.207 2.0

Overall yeast output (g) 3.11

4 Step

m=4.77g m=7.88g
 Difficulties during
the yeast production
Solution
To overcome it, led
and photoresistor
being should perfectly
isolated when
submerged into
medium
The turbidity sensor
and pH sensor could
not work
simultaneously
Due to the wires .
on the led and
photoresistor
being not
perfectly isolated
when submerged
into medium
Thank You
For your attention!