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    Ma Rodelyn Vivar
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    1. The document describes 7 different staining techniques used to identify bacteria: simple staining, negative staining, Gram staining, acid-fast staining, flagella staining, endospore staining, and capsule staining. 2. Each technique uses a different combination of initial and counter stains to color bacteria or structures like flagella, endospores, or capsules in specific ways to facilitate identification under a microscope. 3. The table provided gives examples of bacteria stained with each technique and how they appear, such as Bacillus cereus stained blue with methylene blue using simple staining.

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    1. The document describes 7 different staining techniques used to identify bacteria: simple staining, negative staining, Gram staining, acid-fast staining, flagella staining, endospore staining, and capsule staining. 2. Each technique uses a different combination of initial and counter stains to color bacteria or structures like flagella, endospores, or capsules in specific ways to facilitate identification under a microscope. 3. The table provided gives examples of bacteria stained with each technique and how they appear, such as Bacillus cereus stained blue with methylene blue using simple staining.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    7 views8 pages

    Module 2 Lesson 6 Post Task

    Uploaded by

    Ma Rodelyn Vivar
    1. The document describes 7 different staining techniques used to identify bacteria: simple staining, negative staining, Gram staining, acid-fast staining, flagella staining, endospore staining, and capsule staining. 2. Each technique uses a different combination of initial and counter stains to color bacteria or structures like flagella, endospores, or capsules in specific ways to facilitate identification under a microscope. 3. The table provided gives examples of bacteria stained with each technique and how they appear, such as Bacillus cereus stained blue with methylene blue using simple staining.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    of 8

    MODULE 2

    LESSON 6
    POST TASK
    In tabulated form, complete the table below:

    Staining
    Initial stain Counterstain Bacteria being stained
    technique

    1. Simple Basic dyes such


    as Crystal
    None
    Staining Violet,
    Methylene
    Blue, Safranin,
    and Carbol
    Fuchsin Bacillus cereus stained with
    Methylene Blue
    Staining Initial stain Counterstain Bacteria being stained
    technique

    2. Acidic dyes None


    Negative like
    Staining Nigrosin

    Bacillus megaterium negatively-


    stained with Nigrosin
    (colorless against dark background)
    Staining Initial stain Counterstain Bacteria being stained
    technique

    3. Crystal Safranin
    Differential Violet
    Gram
    Staining

    Pinkish Red-Escherichia coli


    (Gram-negative bacteria)
    Blue-Staphylococcus aureus
    (Gram-Negative bacteria)
    Staining
    Initial stain Counterstain Bacteria being stained
    technique

    4. Carbol Methylene
    Differential Fuchsin Blue
    Acid-Fast
    Staining

    A. Blue Stain: Nocardia Coccidian


    Parasites: Cryptosporidium
    (Non-Acid Fast)
    B. Bright red to intensive purple:
    Mycobacterium tuberculosis
    (Acid-Fast)
    Staining Initial stain Counterstain Bacteria being stained
    technique

    5. Flagella Tannic Acid Pararosaniline


    Staining
    (Special
    Staining)

    Salmonella typhi. having a peritrichous


    flagella
    Staining Initial stain Counterstain Bacteria being stained
    technique

    6. Endospore Malachite Safranin


    Staining Green
    (Special
    Staining)

    Red-colored vegetative Bacilli with


    green-colored endospores
    Staining
    Initial stain Counterstain Bacteria being stained
    technique

    7. Crystal 20%
    Capsule Violet Copper Capsule

    Staining sulfate
    (Special Background
    Cell

    Staining)
    Serratia marcescens
    Capsules that are colorless (clear halos zone)
    between dark purple background of negative
    stain and colored cell.

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