GROUP 3

Ewos, Christian Stefany Bagorio, Dannielle Ashley

Fernandez, Dallin Bambao, Rica Nicolei
Aytona, Sunshine Rianna Ysobelle
BSMLS 2A | T 7:30-10:30

STAINS PURPOSE FIXATION RESULT SECTION

A. MICROORGANISM
1. Gram’s method
2. Gram-Twort stain
3. Modified Brown-Brenn
method
4. Ziehl-Neelsen stain
5. Fite stain
6. Auramine-rhodamine Extremely sensitive and highly 10% neutral buffered formalin Acid-fast organism: Reddish- 4-5 µm paraffin
fluorescent technique specific method for mycobacteria yellow fluorescence

Background black
7. Toluidine blue stain To stain Helicobacter Formalin Helicobacter: dark blue against a Paraffin
variably blue background
8. Cresyl violet acetate method To stain Helicobacter Formalin Helicobacter and nuclei: blue Paraffin
violet

Background: shades of blue-

violet
9. Dieterle method To stain Spirochetes and Formalin L. pneumophilia, spirochetes, Paraffin
Legionella bacteria: Brown to black

Background Pale yellow or tan

10. Levaditi’s method To stain Spirochetes Formalin Spirochetes: black Paraffin
11. Warthin-Starry method
12. Steiner and Steiner
microwave procedure
13. Grocott methenamine silver
stain
14. Lendrum’s Phloxine-
Tartrazine method
15. Orcein method
16. Giemsa stain For blood and marrow parasites, B5 or Zenker’s fixative Protozoa and some other Thin (3m) paraffin sections
inclusion conjunctivitis, organism: dark blue
toxoplasma, spirochetes, and Background: pink-pale blue
other bacteria Nuclei: blue
B. HISTOCHEMISTRY AND
GROUP 3
Ewos, Christian Stefany Bagorio, Dannielle Ashley
Fernandez, Dallin Bambao, Rica Nicolei
Aytona, Sunshine Rianna Ysobelle
BSMLS 2A | T 7:30-10:30
OTHERS
1. Enhanced polymer one step Cell proliferation markers 70 molecules of enzyme; 10 Frozen section
staining molecules of antibody attached
to a spine molecule
2. Peroxidase-anti-peroxidase Binding of soluble peroxidase- Formalin Rabbit anti-human IgG Paraffin section
(PAP) technique antiperoxidase to unconjugated
primary antibody
3. Avidin-Biotin complex Amplify signals in Colorimetric end products Paraffin or frozen section
technique immunohistochemistry
4. Direct immunofluorescence Detection of antibodies No fixative Apple-green fluorescence when Thin (2-5m) cryostat sections
technique for solid tissue fluorescein is used as fluoro-
biopsies chrome
Orange-red fluorescence with
rhodamine conjugates
5. indirect immunofluorescence
technique
6. Frozen section
immunocytochemistry
7. Frozen section
immunofluorescence
8. Direct immunoperoxidase
method
9. Paraffin wax section
immunoperoxidase technique