NUTRITIONAL, AND METABOLIC CHARACTERIZATION OF

STREPTOZOTOCIN-INDUCED DIABETES IN MALE

WISTAR RATS WITH JUGULAR AND INTRAGASTRIC
CANNULATION
N. Qomariyah 1,3
, J.K. Kruit , and G. van Dijk
2 1

1
Department of Neurobiology, GELIFES, University of Groningen, The Netherlands
2
Department of Pediatrics, University Medical Center Groningen, The Netherlands
3
Department of Biology, Faculty of Mathematics and Natural Sciences,
The State University of Surabaya, Indonesia
INTRODUCTION
Effects on nutritional status
• Streptozotocin (STZ) is particularly toxic for pancreatic beta cells
• STZ is frequently used for studying experimental diabetes
Gambar file 3 dan 4
• Data on dosing and subsequent characterization of diabetes is limited
• To investigate STZ dose-response characteristics in male Wistar rats by
studying nutritional, metabolic and cognitive aspects of diabetes
development STZ dose 50 and 55 mg/kg causes significant loss of
body weight and fat content.
METHODS

Effects on metabolic status

Gastric,
jugular
cannulation Group of
treatment:
(mg/kg-bw) Gambar file 5 dan 6

Control
STZ 40
Recovery STZ 45
STZ 50
STZ 55

STZ causes dose-dependent baseline

hyperglycemia and insulin insufficiency.
• STZ doses dissolved in citrate buffer 0.1M (pH 4.5), injected
intraperitoneally (day 0, induction)
• Observation of diabetes development: 21 days Discussion
• Parameters: nutritional (food & water intake), metabolic (Fasting blood • Increased food intake on diabetic groups caused by the reduction of
glucose, IgGTT, IvGTT & insulin levels), cognitive function (behavioural glucose utilization in peripherals tissue and
test), and body fat analysis • Increasing water consumption in diabetic rats caused by increasing
concentration of glucose, urea and other solutes in blood, and as
RESULTS
compensation lost a lot of water by urination.
 The lost body weight as a result of the breakdown of fat storage for
Effects on nutritional status
(gluconeogenesis). Therefore, fat mass in the body or skin will
decreased

Diisi gambar file 1 dan 2 Conclusions

Intraperitoneally injected STZ in doses of 40-45 mg/kg-bw produces a
mild form of diabetes in male Wistar rats with indwelling catheters, which
can be used for subsequent investigation of diabetes etiology and
STZ causes dose-dependent increases in food/water intake treatment.

Email: nurqomariyah@unesa.ac.id