STANDARDIZATION OF HERBAL DRUGS

BHARATI VIDYAPEETH COLLEGE OF PHARMACY

KOLHAPUR

STUDENT NAME : MR. KAMLESH BALIRAM KAMBLE

ROLL NO : 24

GUIDE NAME: MRS. R.R.JARAG.

SR.
No.
Content

1. Abstract

2. Introduction

3. Principle Of HPTLC

4. HPTCL Analysis Of Ursolic Acid In Diospyros Melanoxylon

5. Application

6. Conclusion
 HPTLC INSTRUMENTATION OVERVIEW

ABSTRACT
 HPTLC is one type of planar chromatography.
 Now a day HPTLC is indispensable of analysis in the natural products. It has been preferred over TLC and HPLC.
Because HPTLC is independent of sample application, chromatogram development, detection, etc., it is not only
instrumental TLC but entire concept that include widely standardize methodology based on validated method.
 This type of analysis is controlled by Win CATS and Vision CATS software’s. The present report is helpful to carrying
out analysis of phytochemicals (analytes) present in the herbal drugs or natural products.
 In this project work, we report a review on HPTLC of various analyses so that the students get compilation of the
analysis of phytochemicals reported in the reputed literature. The literature suggests that some of the phytochemicals can
be analyzed under UV light of short (254 nm) and long (366 nm) wavelengths for e.g., ursolic acid, lupeol, withferin A,
andrographolide, oleinolic acid, eugenol, ferolic acid, B sitosterol etc.
 The literature also include the applications of HPTLC and its methodology. It include a brief introduction of
chromatograms. The tabulated data on the profiles of the phytochemicals analysis include acuracy, precision, sensitivity
(LOQ, LOD), linearity range, linear regression equation, Rf value, robustness. It also reports use of different spraying
reagents used in HPTLC analyses.
 INTRODUCTION

 Planar Chromatography as opposed to column chromatography (e.g. GC, HPLC) utilizes a flat
(planar) stationary phase for separation.
 HPTLC is an entire concept that includes a widely standardized methodology based on scientific
facts as well as the use of validated methods for qualitative and quantitative analysis.
 The most advanced form of instrumental TLC is commonly called high performance thin- layer
chromatography (HPTLC), but the term does not simply imply instrumental TLC on special high
performance layers.
 The possibility of visual evaluation ofseparated samples on the plate is one of the most valuable
aspects of TLC.
 PRINCIPLE
 The HPTLC works on the same principles as TLC such as the principle of separation is
adsorption.
 The mobile phase or solvent flows through the capillary action.
 The analytes move according to their affinities towards the stationary phase (adsorbent).
 The higher affinity component travels slower towards the stationary phase.
HPTLC METHODOLOGY
 Difference between TLC and HPTLC

PARAMETER TLC HPTLC

Chromatography Plate used Hand Made Pre-coated

Sorbent layer thickness 250 µm 100-200 µm

Pre washing of plates Not followed must

Application of sample Manual Automatic

Sample volume 1-10µl 0.2-5µl

Efficiency low High

Spots size 2-4mm 0.5-1mm

 Applications
 Pharmaceutical Industry : quality control, content uniformity, identity/purity
check.
 Food Analysis: quality control, additives, pesticides, stability testing.
 Clinical Application : metabolism studies, drug screening, stability testing etc.
 Industrial Application: process development and optimization, In-process check,
validation etc.
 Forensic : poisoning investigation.
 Finger print analysis.
 CONCLUSION

 As we have seen that Standardization guarantees the content of one or more

active constituents and marker compounds. Basically the plant environment and
their genetic factors could significantly affect the biochemical components of the
plant extract, by which the plants are still most abundant and cost-effective
resource for the drug innovation.
 I analyzed the HPTLC of Ursolic Acid in Diospyros Melanoxylon in which the
instrument CAMAG HPTLC system is to be used and the percentage (%) content
( w/w) of ursolic acid in aerial parts extract is found to be 0.07.
THANK YOU..