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    1. The document studied the effect of different concentrations of dibenzothiophene (DBT) on ferrous iron concentration and bacterial cell growth when incubated with Acidothiobaccilus ferroxidans over 12 days. Higher DBT concentrations led to lower iron concentration and less bacterial growth. 2. Thin layer chromatography was used to analyze the degradation of DBT at different time points when incubated with A. ferroxidans. Over time, 2-hydroxybiphenyl (2-HBP) appeared as DBT degraded. 3. The experiment shows that A. ferroxidans is able to degrade DBT through the formation of 2-HBP as an intermediate product, and higher

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    1. The document studied the effect of different concentrations of dibenzothiophene (DBT) on ferrous iron concentration and bacterial cell growth when incubated with Acidothiobaccilus ferroxidans over 12 days. Higher DBT concentrations led to lower iron concentration and less bacterial growth. 2. Thin layer chromatography was used to analyze the degradation of DBT at different time points when incubated with A. ferroxidans. Over time, 2-hydroxybiphenyl (2-HBP) appeared as DBT degraded. 3. The experiment shows that A. ferroxidans is able to degrade DBT through the formation of 2-HBP as an intermediate product, and higher

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    12 views13 pages

    Presentation 1

    Uploaded by

    print 08
    1. The document studied the effect of different concentrations of dibenzothiophene (DBT) on ferrous iron concentration and bacterial cell growth when incubated with Acidothiobaccilus ferroxidans over 12 days. Higher DBT concentrations led to lower iron concentration and less bacterial growth. 2. Thin layer chromatography was used to analyze the degradation of DBT at different time points when incubated with A. ferroxidans. Over time, 2-hydroxybiphenyl (2-HBP) appeared as DBT degraded. 3. The experiment shows that A. ferroxidans is able to degrade DBT through the formation of 2-HBP as an intermediate product, and higher

    Copyright:

    © All Rights Reserved
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    of 13

    1.

    Ferrous iron estimation (g/L) of different


    concentration of DBT (300mg,400mg&500mg) in
    presence of Acidothiobaccilus ferroxidans (At.f).
    9K + At. F 9K + At. F + DBT 400mg 9K + At. F + DBT 500mg

    day Fe conc (g/L) day Fe conc (g/L) day Fe conc (g/L)

    0 8.33 0 8.33 0 8.33

    2 2.94 2 3.29 2 3.79

    4 0.73 4 0.49 4 0.24

    6 0.24 6 0.24 6 0.24

    8 0.24 8 0.24 8 0.24

    10 0.24 10 10

    12 0.24 12 12
    9
    8.33

    6
    ferrous iron concentration (g/L)

    4 3.79
    3.29
    2.94
    3

    1 0.73
    0.49
    0.24 0.24 0.24 0.24 0.24
    0
    0 2 4 6 8 10 12 14
    9K + At. F Days
    9K + At. F + DBT 400mg 9K + At. F + DBT 500mg
    9K + At. F 9K + At. F + DBT 300mg

    day Fe conc day Fe conc

    0 9.8 0 9.8

    2 7.1 2 7.35

    4 4.41 4 6.12

    6 1.96 6 2.94

    8 0.49 8 1.22

    10 0.12 10 0.24

    12 0.02 12 0.12
    12

    9K + At. F 9K + At. F + DBT(300mg)

    10
    9.8

    8
    7.35
    7.1
    ferrous iron conc.

    6 6.12

    4.41
    4

    2.94

    2 1.96

    1.22

    0.49
    0.24
    0.12 0.12
    0 0.02
    0 2 4 6 8 10 12 14

    day
    2. No of cells (number 10*6)of different
    concentration of DBT (300mg,400mg&500mg)
    in presence of Acidothiobaccilus ferroxidans
    (At.f).
    9K + At. F + DBT
    9K + At. F(control) 9K + At. F + DBT 300mg 9K + At. F + DBT 400mg 500mg

    day no. of cells day no. of cells day no. of cells day no. of cells

    0 3.9 0 3.9 0 3.9 0 3.9

    2 20.88 2 18.82 2 16.82 2 14.29

    4 22.2 4 20.88 4 11.75 4 11.68

    6 29.49 6 16.76 6 7.89 6 4.97

    8 58.44 8 15.1 8 4.41 8 3.99

    10 33.18 10 6.8 10 10

    12 28.27 12 5.1 12 12
    Chart Title

    70

    60
    58.44

    50

    40
    no of cells (10*6)

    33.18
    30 29.49
    28.27

    22.2
    20.88 20.88
    20
    18.82
    16.82 16.76
    15.1
    14.29
    11.75
    11.68
    10
    7.89
    6.8
    4.97 4.41 5.1
    3.9 3.99

    0
    0 2 4 6 8 10 12 14

    Days

    9K + At. F 9K + At. F + DBT 300mg 9K + At. F + DBT 400mg 9K + At. F + DBT 500mg
    3. TLC profiling for degradation of of DBT
    (300mg,400mg&500mg) using Acidothiobaccilus
    ferroxidans (At.f).
    2HBP

    DBT

    DBT DBT +2HBP 2HPB


    2HBP
    Day 0 Day 2 Day 4
    DBT
    2HBP
    Day 6 Day 8 Day 10
    DBT
    OVERALL

    DBT

    2-HBP

    DBT 2-HBP D4-400 D4-500 D8-400 D8-500

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