Micropipettor Instructions:

1. Check that your volume is correct.

2. Put on proper pipet tip.
3. Depress plunger to 1st stop (measured volume).
4. Place tip in solution to be pipetted.
5. Slowly release plunger to full extension to draw up liquid
6. Place tip in destination tube, against inner wall.
7. Depress slowly through 1st stop and to 2nd stop to expel all contents.
8. Release plunger to full extension
9. Dispose of tip.
 Fully extended
plunger 1st stop (measured volume)
2nd stop (blowout)

Volume display
Plunger depressed
to 1st stop
(measured volume)
Plunger depressed
to 2nd stop
(blowout)
 P1000

Measures
100-1000uL
P1000 pipettors, both reading 1000uL P1000 pipettors, both reading 400uL

Note – no 1s digit on Labnet & VWR!

P200 Measures 20-200uL
Note – if measuring 100uL, using a P200 is a better choice than a P1000
P200 pipettor P200 pipettors, both reading 50.5uL

Line to indicate digit

after decimal

Notice – this Eppendorf even shows

one digit to the right of the decimal The Eppendorf brand on the left would be a better
point, it is reading 150.0uL choice for measuring 50.5uL, if you have that option
P1000 pipettors, both reading 378uL P200 pipettors, both reading 21.5uL

Pipettor on the right does not show

a 1s digit separately
 P20
Measures 2-20uL

Notice – different
brands have different
methods of showing a
Line to indicate
decimal point Line to indicate digit digit after decimal
after decimal

New (red) color for

digit after decimal
Serological pipettes
Liquid is pulled up from the
tip, using a manual pump
Serological pipettes

Notice – many pipette brands

are numbered with the
largest number at the tip

0.7mL
1mL

1mL

Pulling liquid up to here

= 2.7mL
Part 1: Learning the ropes
use 15mL conical

Part 2: Small volume pipetting

use rack of 1.5mL microfuge tubes

Part 3: Serological pipette challenge

use small glass test tubes in test tube rack