Laboratory Safety &

Common Laboratory
Equipment and
Apparatus

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

 Augustinian Prayer Before the Class
Leader: When we live in unity…
All: How good and pleasant it is.
Leader: Pray for us, Holy Father Augustine
All: That we may dwell together in peace
Leader: Let us pray
All: God, our Father, your Son promised to be present in
the midst of all who come together in His name. Help us to
recognize His presence among us and experience in our
hearts the abundance of Your grace. Your mercy and peace,
in truth and in love. We ask this through Christ, our Lord.
Amen.

Our Father… USA_Chem_J.Juaneza

Courtesy: D. Loking
 WHAT’S
WRONG
WITH THIS
PICTURE?

DOES RICK,
SARAH AND
ANDREW
PRACTICE
LABORATORY
SAFETY RULES?
Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI
LABORATORY SAFETY RULES

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI
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 Ensure
Appropriate
Laboratory
Conduct

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

 (1) Ensure Appropriate Laboratory
Conduct
a.Wear proper dress code or Personal Protective Equipment
(PPE) when inside the research laboratory. PPE’s may include
laboratory gown, safety goggles, close-toed shoes, gloves and
facial mask. Note also of the following:

i. Always tie back hair that is chin-length or longer.

ii. Make sure that loose clothing or dangling jewelry is secured, or avoid
wearing it in the first place.
iii.Never wear sandals or other open-toed shoes in the lab. Footwear
should always cover the foot entirely.
iv. Never wear shorts in the lab.
v. Wear safety goggles when working with hazardous materials,
equipment, glassware or when heating.
vi. It is best to wear gloves when handling toxic
Prepared or hazardous
By: RAQUEL chemical
D. ESTORQUE MILE-PHYSCI
 (1) Ensure Appropriate Laboratory
Conduct
b. Make sure to wash your hands before leaving the research
laboratory.
c. Observe all safety rules and procedures at all times.
d. Always perform the experiments as directed by the research adviser.
e. Never leave experiments while in progress.
f. No student is allowed to work alone in the research laboratory.
Unauthorized performance of an experiment is strictly forbidden.
g. No student is allowed to take chemicals out of the chemical
stockroom without the instruction from the research adviser or
laboratory personnel. Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI
 THE
PICTURE
SHOWS
THAT…

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

 (1) Ensure Appropriate Laboratory
Conduct
h. Strictly no smoking, eating and drinking in the laboratory.
i. Do not engage in practical jokes or horse play while inside the laboratory.
j. Ensure you are fully aware of your emergency facilities and building’s evacuation
procedures.
k. Make sure you know where your lab’s safety equipment such as first aid kit, fire
extinguisher, eye wash stations and safety showers is located and how to properly
use it.
l. Know emergency phone numbers to use to call for help in case of an emergency.
m. Make sure you are aware of where the exit doors are in your laboratory room.
n. If there is a fire drill, be sure to turn off all electrical equipment and close all
containers.
Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI
 (1) Ensure Appropriate Laboratory
Conduct

o. Always work in properly-ventilated areas.

p. Report all injuries, accidents and broken glassware to your
research adviser even if the incident seems small or unimportant.
q. If you have been injured, yell out immediately and as loud as
you can to ensure you get help right away.
r. If you notice any unsafe conditions in the lab, let your research
adviser or lab personnel know as soon as possible.
s. If you are the last person to leave the lab, make sure to lock all
Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI
the doors and turn off all ignition sources.
 Chemical
Safety
Rules

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

 (2) Chemical Safety Rules
a. Every chemical should be treated as hazardous or dangerous.
b. Never taste, touch or smell a chemical directly to your nose.
c. Never place the container directly under your nose and inhale
the vapors.
d. Do not allow any solvent to come in contact with your skin.
e. Never mix or use chemicals not called for in the laboratory
experiment.
f. Never point the open end of a test tube containing a substance
to yourself or others.
g. Never fill a pipette using your mouth. Always use rubber
aspirator.

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

 (2) Chemical Safety Rules
h. Check the bottle label to verify if it is the correct
substance before using it.
i. All chemicals should always be clearly labeled with the
name of the substance, its concentration, and its
amount.
ii. Before removing any contents of chemical from a bottle,
make sure to have read the label twice or more.
i. If you transfer chemicals from their original containers,
label chemical containers as to the contents, concentration,
and amount.
j. Do not put unused chemicals back into their original
container.
k. Always use a spatula or spoon to remove a solid reagent
from a container. Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI
(2) Chemical Safety Rules

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

 (2) Chemical Safety Rules
l. Do not directly touch any chemical with your bare hands.
m. Never use a metal spatula when working with peroxides. Metals
will be decomposed explosively with peroxides.
n. Flammable and volatile chemicals should only be used under the
fume hood.
o. Hold containers away from the body when transferring a chemical
or solution from one container to another.
p. Add concentrated acid to water slowly. Never add water to a
concentrated acid

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

 (2) Chemical Safety
q. Use the laboratory chemical fume hood when
Rules
there is a possibility of release of toxic chemical
vapors, dust or gases. When using the hood, the
sash opening should be kept at a minimum to
protect the user and to ensure efficient
operation of the hood.
r. Keep your head and body outside of the hood
face. Chemicals and equipment should be
placed at least six inches within the hood to
ensure proper air flow.
s. When transporting chemicals (250 mL or
more), place the chemical in a secondary
container or bucket (rubber, metal or plastic)
designed to be carried and large enough to hold
the entire contents of the chemical.
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🗸
🗸

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RULES AND REGULATIONS ON
THE USE OF LABORATORY

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

 PROPER USE OF LABORATORY
FACILITIES
1. Apparatus should be kept clean and dry. Cleansing solution should be used for
obstinate stain.
2. All laboratory tables, which the students have used, should be cleaned before they
leave, with no pieces of papers or the solid materials such as matchsticks, broken
glass tubing, etc. clogging the water drainage or left in the sink.
3. All solid waste must be thrown into the garbage can.
4. Any student who needs to get some apparatus and or other accessories from their
lockers outside of their schedule or do overtime work should accomplish properly
the permit provided for such purpose by the stockroom.
5. At the end of semester or term after all apparatus have been returned lockers. Any
locker found locked, a day after the end of the semester, shall be opened and all
contents shall be taken.

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

 RULES GOVERNING BREAKAGES
1. Breakages incurred during the semester or term
should be immediately reported to the
instructors who in turn should require the group
to report to the stockroom personnel.
2. Apparatus purchased elsewhere to replace
broken ones should be accompanied by
corresponding official invoice from the store
where it was purchased.
3. Payment for breakages incurred by any individual
member of a group must be shared equally by all
members.

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

 Issuance of Apparatus and Chemicals
1. Students are grouped in a group of 4-5 students per group. As a group, each group need to
comply all the group requirements.
2. Each student are required to complete the individual requirements.
3. Instructors should submit to the Stockroom a copy of the experiment, a meeting before
the conduct of the experiment.
4. Each group should assign a group leader. Only the group leader or the member(s)
designated by the group leader is allowed to get the apparatus to be assisted by the other
group members.
5. Chemicals needed by the each group are place in chemical trays and issued by stockroom
staff on the day of the experiment.
6. Temporary apparatus are issued to each group. The apparatus temporarily issued must be
returned to the stockroom fifteen (15) minutes before the end of the laboratory period. A
fine is imposed to the group that fails to return the apparatus.
Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI
 Rules and Regulations Governing the Returning of
Apparatus

1. All glassware must be clean and dry inside and outside. Cleansing solution
shall be provided by the stockroom for stains resistant to detergent and
water.
2. The stopcocks and/covers of all burettes , separatory funnels, and other glass
stoppered apparatus must be dried and rubbed lightly with vaseline.
3. The water jackets of condensers should be thoroughly washed with detergent
and water or cleansing solution with necessary , and then dried.
4. Sand baths, test tube holders, cork borers, iron clamps, and holders, crucible
tongs, iron rings, nickel, crucibles, and metal apparatus must be free from
rust, wiped dry and rubbed lightly with vaseline.
Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI
 Rules and Regulations Governing the Returning of
Apparatus

5. All water baths and every piece of the rings must be scrubbed clean with
steel wool, sand or sandpaper, wiped dry and rubbed lightly with vaseline.
6. All rubber tubings and test tubes racks should be thoroughly with
detergent and dried before returning.
7. All porcelain apparatus must be washed thoroughly with detergent and
water. Use cleansing solution to remove stains resistant to detergent and
water.
8. All retained apparatus be returned together with the permanent
apparatus.

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

 Rules and Regulations Governing the Returning of
Apparatus

9. All extra apparatus of the groups or individuals must be

returned to the stockroom together with retained and permanent
apparatus to facilitate returning of the said items to the respective
owners.
10. All apparatus must be returned in scheduled time. If the class
has not finished the returning of apparatus on the assigned
schedule, the teacher may request for another schedule. No
apparatus shall be accepted by the stockroom personnel one day
after the scheduled/rescheduled returning of the said class.

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

 Rules and Regulations Governing the Returning of
Apparatus

11. All lockers and drawers must be cleaned and unlocked

immediately after all the apparatus have been returned. If these
lockers and drawers are found closed at the end of the semester,
there shall be forcibly opened and the contents taken.
12. ALL LABORATORY ACCOUNTS MUST BE PAID BEFORE TAKING
THE FINAL EXAMINATIONS. NO STUDENT SHALL BE ALLOWED TO
TAKE THE FINAL EXAMINATIONS UNLESS HE OR SHE PAID THE
BREAKAGE INCURRED.

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

PROTECTIVE PERSONAL EQUIPMENT (PPE)

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LABORATORY
SAFETY CHART

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

 NFPA Hazard Diamond
What is the NFPA Hazard Diamond?

• The NFPA 704 Diamond,

commonly referred to as the
NFPA Hazard Diamond, provides a
system for identifying the specific
hazards of a material and the
severity of the hazard that would
occur during an emergency
response.
• The system addresses the health,
flammability, instability, and
special hazards presented from
short-term, acute exposures that
could occur as a result of a fire, spill,
or similar emergency.
Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI
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 HAZARD
SYMBOLS

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

LABORATORY
Equipment
and
Apparatus
Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI
 TEST TUBE
• A thin, cylindrical
container,
• usually made of glass
• Used to hold and mix
liquids.

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

Volumetric Flasks
• These flasks help measure liquids
• more accurate than common
measuring cups or flasks.
• This is because volumetric flasks
can hold a precise amount of
liquid.

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

Beaker
• A glass container with a
pouring lip.
• Used to hold, mix or
heat substances.

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

 Watch Glass
• A watch glass is just a round
piece of glass that is slightly
concave/convex (think of a lens).
• It can hold a small amount of
liquid or solid.
• They can be used for
evaporation purposes and also
can function as a lid for a beaker.

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

Wire Gauze
Used to support a
container, such as
beaker on a ring stand
while it is being
heated.

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

 Ring Stand
Used to hold or clamp
laboratory glassware
and other equipment
in place, so it does not
fall down or come
apart.
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Graduated Cylinder
Used to measure a
precise volume of a
liquid.

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

Funnel
Used to transfer
liquids or fine
grained materials
into containers with
small openings.

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

Burette
Used for
dispensing an
accurate volume of
a liquid.

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

Evaporating Dish

Used to heat
liquids for
evaporation.

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

Wash Bottle
Used to rinse pieces
of glassware and to
add small quantities
of water.

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

Fume Hood

• The purpose of a chemical

fume hood is to prevent
the release of hazardous
substances into the general
laboratory space
• by controlling and then
exhausting hazardous
and/or odorous chemicals.

Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI

Dropper/ Pipette
• A thin, tapering glass or
plastic tube
• used to eject or suck in a
small amount of fluid.

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 Augustinian Prayer Before the Class
Leader: Our help is in the name of the Lord..
All: Who made heaven and earth.
Leader: Let us pray
All: God, the desire of every human heart, You, moved St.
Augustine to seek restlessly for truth and peace. Touch our
hearts with His burning desire for wisdom for the Word
made flesh. We ask this through Christ, our Lord. Amen.

Our Father…

USA_Chem_J.Juaneza
Courtesy: D. Loking
Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI
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Prepared By: RAQUEL D. ESTORQUE MILE-PHYSCI