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    Mira cle Star
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    The document provides 4 methods to test for the presence of proteins in a food sample: 1) The biuret test uses copper sulfate and caustic soda, which produces a violet color if proteins are present. 2) Another test uses nitric acid, producing a yellow color for proteins. 3) The millions test uses mercuric sulfate, sodium nitrite, and sulfuric acid, with a brick red color indicating proteins. 4) Copper sulfate alone also produces a violet color with proteins in the biuret test.

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    The document provides 4 methods to test for the presence of proteins in a food sample: 1) The biuret test uses copper sulfate and caustic soda, which produces a violet color if proteins are present. 2) Another test uses nitric acid, producing a yellow color for proteins. 3) The millions test uses mercuric sulfate, sodium nitrite, and sulfuric acid, with a brick red color indicating proteins. 4) Copper sulfate alone also produces a violet color with proteins in the biuret test.

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    7 views17 pages

    Macromolecules Flashcard

    Uploaded by

    Mira cle Star
    The document provides 4 methods to test for the presence of proteins in a food sample: 1) The biuret test uses copper sulfate and caustic soda, which produces a violet color if proteins are present. 2) Another test uses nitric acid, producing a yellow color for proteins. 3) The millions test uses mercuric sulfate, sodium nitrite, and sulfuric acid, with a brick red color indicating proteins. 4) Copper sulfate alone also produces a violet color with proteins in the biuret test.

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    of 17

    MACROMOLECULES

    QUIZ

    Direction: identify which of the following


    chemical reactions shows hydrolysis and
    dehydration reaction?
    LIPIDS
    The presence of proteins in given foodstuff can be tested as
    follows:
    Method 1: To test protein in the food we need a solution of
    copper sulfate and caustic soda.
    - First we grinded the food sample finely (for example almonds).
    - Then, we put it in a clean test tube followed by the addition of
    10 drops of water.
    - Added 2 drops of copper sulfate solution and shook it well.
    - Added 10 drops of caustic soda solution and shook it well.
    - The Violet color indicates the presence of proteins.

    Method 2: Biuret test –


    - First we added the given sample food.
    - We then added aqueous copper sulfate.
    - The violet coloration confirms the presence of protein.
    Method 3: Xanthoproteic test –
    - First we added the given sample food.
    - We then added the nitric acid.
    - The yellow color solution confirms the presence of proteins.
    Method 4: Millions test –
    - First we added the given sample food.
    - We then added the mercuric sulfate in the presence of sodium nitrite and sulfuric acid.
    - The brick red color solution confirms the presence of proteins.

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