O. M.

Marzeiev Institute for Hygiene

and Medical Ecology, Academy of EURO-ECO 2011
Medical Sciences of Ukraine
Hannover
ECOLOGICAL AND HYGIENIC ASPECTS Environmental,
OF SOIL PROTECTION PROBLEMS Engineering -
N.M. Koval Ph.D. Economic and
Legal Aspects for
Sustainable Living

Bioremediation or acceleration
methanogenesis – choice of
earth. Stand lecture

Expert
Vitalii S. Prokhorov
Ph.D.
Ukraine
 Climate change is not a hoax.
The hoax is that we are really
doing something about it.

2002 2010 2011

Methanogenesis is an ancient
metabolism that originated on
the early anoxic Earth.
More Coal?
More Nuclear?
What about Less?
How about less
pollution and
more solutions.
Methane is 21 times more potent
than carbon dioxide in trapping
heat in the atmosphere.

For schoolboys
 For the Drivers
Small box of transmissions
For Biottechnologists
process
For Chemists
Transformations
 Transfer 8 electrons
(Methanogenesis or biomethanation)

For physicists
For Biologists
For environmentalists
For microbiologists
An invasion of armies can
be resisted, but not an idea
whose time has come
 Archaea - The Microorganism
Archaea were first identified by Carl Woese, PhD,
of the University of Illinois in 1975.
It took thirty years for the scientific community
to accept his discovery. Archaea are now the
most studied microorganism for their value in
medicine, industry and bioremediation.
Archaea are not Bacteria
Archaea and bacteria are similar
in size and shape, however
Archaea are not bacteria.
Neither have a cell nucleus, or
other organelles within their
cells. They differ in that bacteria
cell walls have a fatty, lipid core
while Archaea has a glycerol cell
wall.
Additionally, Archaea have a
complex gene control system
using histones and RNA similar
to plants and animals.
Archaea and Bacteria
Archaea consume hydrocarbons
via direct metabolism.

Because archaea came into existence when the

only available food source was hydrocarbons,
they developed enzymes to cut one carbon at a
time and thus can attack most any molecule no
matter how complex.

Archaea and bacteria work together in a

syntropic relationship. Bacteria and Archaea
share cell membranes. As bacteria accumulates
waste, the waste and the energy to break it
down are sent to Archaea. Then Archaea
breaks the waste down and sends back CoA
which provides more reaction sites in the
bacteria. As a result, waste breakdown is
accelerated.
 Archaea, Miracle microbes

Archaea are Methanogens

Methanogenesis or biomethanation is the
formation of methane by microbes known as
methanogens. Organisms capable of
producing methane have been identified only
from the domain Archaea. Methanogenesis
is the final step in the decay of organic
matter. Archaea is safe having been tested
against strict EPA standards. It has the ability
to get under roads and buildings, it enhances
indigenous bacteria, and is uniquely able to
deal with any hydrocarbon contamination. It
is the last step in UST site cleanup.
 Methanosarcina barkeri
Ribbon diagram of a subunit of the
Methanosarcina barkeri monomethylamine
methyltransferase hexamer (α helices: green; β
sheets: cyan; random coil: yellow). The atoms of the
UAG-encoded residue are shown as ball-and-stick
models and are colored by their elements, with
carbon as gray, nitrogen as blue, and oxygen as red.

Pyrrolysine is an amino acid used by some

methanogenic (organisms that produce
methane) archaea in enzymes that are part of
their methane-producing metabolism.
N6-[(2R,3R)-3-methyl-3,4-dihydro-2H-pyrrol-2-ylcarbonyl]-L-lysine.
Wonders of enzymes
Simple complications
We adopt a fermenter
at nature
We prepare
a cocktail
We do an useful injection
Only when the last
tree has died, the last
river has been
poisoned and the last
fish has been caught
will we realize that we
cannot eat money
Don't think, think about not thinking.
You never know what
question you've asked until
you hear the answer.
 We could have saved
the Earth but we were
too damned cheap.

bioremediation
To know a diagnosis – 90%
success
On dumps build stadiums
 It is in every case
necessary to
count
investments

Accelaration methanogene-
sis
70
60
50
40
CH4 %

30
20
10
0
1 3 5 7 9 11 13 15
 A n i n v e s t m e n t o p e r a ti o n i s o n e w h i c h , u p o n t h o r o u g h a n a l y s i s
promises safety of principal and an adequate return .

Practical science searches the ways of

introduction of innovations, and business
searches where he is cheated!
We have a paper
 Thank you
for your
attention
An abundance of information yields a poverty of attention.

Where Are the Customers' Yachts?

Expert
Vitalii S. Prokhorov
Ph.D.
Ukraine
Tel: +380503009902
expertpv@ya.ua
 At the core of all well-
founded belief, lies belief
that is unfounded………

Questions ???