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    Genetic engineering is the process of manipulating genes from different species and inserting them into an organism's genome to give it desired traits. This involves using recombinant DNA technology to cut and join DNA from different sources. For example, genes encoding pesticidal proteins from Bacillus thuringiensis bacteria have been inserted into corn plants, creating Bt corn which is resistant to certain insect pests. The process involves cutting DNA with restriction enzymes, joining the foreign gene to a vector like a plasmid, transferring the recombinant DNA into a host cell, and screening for cells that express the new gene.

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    Genetic engineering is the process of manipulating genes from different species and inserting them into an organism's genome to give it desired traits. This involves using recombinant DNA technology to cut and join DNA from different sources. For example, genes encoding pesticidal proteins from Bacillus thuringiensis bacteria have been inserted into corn plants, creating Bt corn which is resistant to certain insect pests. The process involves cutting DNA with restriction enzymes, joining the foreign gene to a vector like a plasmid, transferring the recombinant DNA into a host cell, and screening for cells that express the new gene.

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    1 views19 pages

    Genetic-Engineering Part 1

    Uploaded by

    Wattpadpurposes
    Genetic engineering is the process of manipulating genes from different species and inserting them into an organism's genome to give it desired traits. This involves using recombinant DNA technology to cut and join DNA from different sources. For example, genes encoding pesticidal proteins from Bacillus thuringiensis bacteria have been inserted into corn plants, creating Bt corn which is resistant to certain insect pests. The process involves cutting DNA with restriction enzymes, joining the foreign gene to a vector like a plasmid, transferring the recombinant DNA into a host cell, and screening for cells that express the new gene.

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    of 19

    GENETIC ENGINEERING

    Describe the process of Genetic Engineering


    DNA
    (Deoxyribonuclei
    c Acid)
    Bacteria
    Recombina
    nt DNA
    (rDNA)
    Flvar Savr
    Bt COrn / Bt Maize
    Bt Eggplant
    Genetic
    Engineering

    Genetic engineering is the process of


    using recombinant DNA (rDNA)
    technology to alter the genetic
    makeup of an organism. Genetic
    engineering involves the direct
    manipulation of one or more genes.
    Most often, a gene from another
    species is added to an organism's
    genome to give it a desired
    phenotype.
    Classical plant breeding uses
    deliberate interbreeding
    Classical (crossing) of closely or distantly
    related individuals to produce
    Breeding new crop varieties or lines with
    desirable properties. Plants are
    crossbred to introduce traits/genes
    from one variety or line into a new
    genetic background.
    The modification in Genetic Engineering may
    involve the following;

    Genetic - Introduction of new traits in organisms


    Engineering - Enhancement of present trait by
    increasing the expression of desired gene
    - Enhancement of a present trait by
    disrupting the inhibition of the desired genes’
    expression
    Goal: Solve problem
    on pests

    Pest resistant corn


    Bt corn
    Pest resistant corn Non-Pest resistant
    corn
    Bacillus thuringiensis(Bt)
    • is a species of bacteria that
    lives in soil

    • It makes proteins that are


    toxic to some insects when
    eaten, but not others
    Process of Genetic
    Engineering

    1. cutting or cleavage of DNA by


    restriction enzymes (REs)
    2. selection of an appropriate
    vector or vehicle which would
    propagate the recombinant DNA
    (eg. circular plasmid in bacteria
    with a foreign gene of interest)
    3. ligation (join together) of the
    gene of interest (eg. from animal)
    with the vector (cut bacterial
    plasmid)
    4. transfer of the recombinant
    plasmid into a host cell (that 5. selection process to screen
    would carry out replication to which cells actually contain
    make huge copies of the the gene of interest
    recombined plasmid)

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