QUILINGAN, CEMBERG E. ESPLANA, JHAMAYCA DAIGDIGAN, ERICA JOY
GENETIC ENGINEERING - Is the process of changing the DNA (Deoxyribonucleic
Acid) in living organism to create something new. RECOMBINANT TECHNOLOGY - Is the technique of combining two DNA sequences from different sources. One goal of this technique is cloning or producing a particular gene for analysis or mass production of a medically useful protein product. This technique uses a plasmid usually from bacteria as vector or delivery vehicle of the desired trait. A plasmid is a small, circular, double-stranded DNA molecule. When a bacterium divides, all the plasmids contained within the cell are copied such that each daughter cell receives a copy of each plasmid.
PROCESS OF GENETIC ENGINEERING
Locating an organism with a specific trait and extracting its DNA. Cloning a gene that controls the trait. Designing a gene to express in a specific way. Transformation, inserting the gene into the cells of a crop plant. Cross the transgene into an elite background.
GENETICALLY MODIFIED ORGANISM (GMO) - Are organisms or microorganisms
whose genetic material have been altered to contain a segment of DNA from another organism. ADVANTAGES OF GMO ● Genetic engineering has a wide application in engineering and in agriculture. ● Because of genetic engineering, plants become resistant to pests, weeds, and that crops are grown with more nutritional values. ● Pest resistant plants- scientist were able to develop pest- resistance crops by introducing bacterium, Bacillus thuringiensis (Bt) to plants. ● Growing crops in different seasons and conditions can be done. Plants that do not grow in warm weather can be modified to fit the different types of weather. DISADVANTAGES OF GMOS ● GMO crops increase pesticides used. ● GMO crops have created superweed. ● GMO have toxic or allergenic effects on laboratory animals based on studies. ● GMO is not precise technology that will continue to deliver unpleasant surprises. Ang genetic engineering ay ang proseso ng pagmamanipula ng DNA ng isang organismo upang baguhin ang mga katangian o katangian nito. Magagawa ito sa maraming paraan, gaya ng pagpasok ng gene mula sa isang species patungo sa isa pang species, o sa pamamagitan ng pagtanggal o pag-disable ng ilang partikular na gene. Ginagamit ang genetic engineering sa iba't ibang larangan, kabilang ang medisina, agrikultura, at pananaliksik. Ito ay may potensyal na lutasin ang maraming problema at mapabuti ang buhay ng mga tao, ngunit ito rin ay naglalabas ng mga alalahanin sa etika. Madalas itong ginagawa gamit ang genetic engineering techniques, gaya ng pagputol at pag-splice ng DNA mula sa iba't ibang source at pagpasok nito sa isang host organism. Ang nagresultang organismo, na naglalaman ng bagong genetic na materyal, ay tinatawag na recombinant organism. ang ibig sabihin niya ay ang genetic makeup ng organismo ay nabago sa paraang hindi natural na nangyayari. Ang layunin ng genetically modifying ng isang organismo ay madalas na magpakilala ng isang bagong katangian o katangian na maaaring maging kapaki-pakinabang, tulad ng pinahusay na ani ng pananim o paglaban sa mga peste. Gayunpaman, ang paggamit ng mga GMO ay isang kontrobersyal na paksa, na may ilang mga tao na nagpapahayag ng mga alalahanin tungkol sa kanilang mga potensyal na epekto sa kapaligiran at kalusugan ng tao.
A Seminar ON Genetic Engineering and Gene Cloning BY Ayinde Samson MATRIC NUMBER: 168859049 Department of Biochemistry Faculty of Science, Ekiti State University. JUNE, 2021