QUALITATIVE ANALYSIS OF STARCH

Presented by : Guided by:

Rachitha K N Yashwanth sir
1st year MSc Chandini mam
Department of food science Department of food science
and nutrition and nutrition
 CARBOHYDRATES

 Carbohydrates are derivatives of aldehydes and ketones .

 Carbohydrates mainly consists of C , H and O in the ratio 1:2:1
 Carbohydrates are mainly classified into three types:
Monosaccharide: ex : glucose , fructose
Disaccharides : ex : sucrose , maltose
polysaccharides : ex : starch, cellulose
 QUALITATIVE ANALYSIS OF STARCH

There are different tests for the analysis of carbohydrates/starch:

 Molisch test
 benedict’s test
 Barfoed’s test
 Tollen’s test
 Iodine test
 Fehling’s test
 BARFOED’S TEST

 Test to distinguish monosaccharide / disaccharide.

 Principle : oxidation of reducing sugar by copper ion to form a carboxylic acid
and copper oxide, results in formation of red Coloured precipitate.
 Procedure : 1 ml of sample + 2 ml of barfoed’s solution , boil in water bath for
1 min , red color/precipitate is formed.
 Monosaccharide: result in 1-5 minutes
 Disaccharide : result in 7-9 minutes.
Reaction of barfoed’s test
 TOLLEN’S TEST

 To know the given compound is reducing /non reducing

 Also to check the presence of aldehyde /ketone.
 Reagent : alkaline ammonical silver nitrate
 Procedure : take two dry test tubes and add 1 ml of
sample in one test tube and 1 ml of distilled water
in another as blank + 2ml of tollen’s reagent to both
test tubes and keep both the test tubes in water bath for 1 min
 Observe the silver mirror on sides of the test tubes
indicates the presence of aldehyde.
Tollen ‘s test reaction
 REFERENCE
• (PDF) Qualitative analysis of Carbohydrates - Academia.edu
• https://www.academia.edu › ..Experiment 6: Qualitative test Test
for Carbohydrates
• https://www.mlsu.ac.in › econtents › 22
• Laboratory Report on Biochemistry of Carbohydrates - Studocu
• https://www.studocu.com › .
THANK YOU