Faculty of Pharmacy

Pharmaceutics Department

Research Project
RSPT1.9

A Sustainable Approach for a Novel Greenly-

Synthesized Nano Pharmaceutical System for
Wound Healing
 Prepared by

Cheri Ussama - 200791

Jouliana Ashraf -201111
Nermin Hussein -200957
Salma Mohamed -201515

Under the Supervision of

Dr. Omar Elkady

Dr. Dina Saeed
A.L. Maysoon Mostafa
T.A. Aya Ahmed Maher
 Outline
01 Wounds 02 Wound Care and
Management

03 Carrier of Choice 04 CDs Precursor

Aims and
05 Drug 06 Objectives

07 Action Plan
 01
WOUNDS
 Wounds
burden

Physical Psychological
Challenges in the Wound Healing Process

Delayed Scar
healing
Infections formation

Wound
Pain management
management complexity
Challenges in the Wound Healing Process

Infections
 02
Wound Care and
Management
Conventional Herbal Treatment
● Aloe vera
● Turmeric
● Garlic
● Thyme
 Drugs
● Antibacterial agents
● Anti-inflammatory agents
● Analgesics
 Current Advanced Approaches
● Liposomes ↓ Loading
capacity
● Niosomes
● Exosomes
● Polymeric micelle
● Nanoemulsions ↓ Stability ↓ Biocompatibility

Tedious
preparation
 03
Carrier of
Choice
Carbon Dots
Carbon Dots
● Zero-dimension
● Highly fluorescent
● Eco-friendly
● Biocompatible
● Easy to synthesize
● Low cytotoxicity
Damaging the
bacterial cell Promoting fibroblast
membrane and cell proliferation

Generation of rich Photodynamic

intracellular ROS therapy

Biological sensor
 04
Carbon Dots
Precursor
 Garlic as Precursor
Antioxidant
Antimicrobial

Anti-Inflammatory
 05
Drug
 Indications

Us
es
Drug of choice in Wound Healing

Anti-Oxidant
& ROS
Scavenger

Mechanism
of

y
Actions

tor
lam -
Me tor
Ch

inf Anti
ma
ela
tal
Repurposing of Alpha Lipoic Acid

Traditional use
for chronic
diseases

Promising wound
healing activity
 06
Aim and
Objectives
 Garlic
(Precursor)

Synergism
Alpha Carbon
Lipoic Acid Dots
 Aim
To develop and investigate an innovative greenly-synthesized
nanosystem, designed for topical delivery with the potential to enhance
wound healing through a synergistic drug repurposing strategy.

Objectives
● Synthesis and ● In vitro and ex vivo
● Physico-chemical
characterization of assessment of the
characterization of the
highly fluorescent developed system for
drug-loaded carbon
carbon dots derived potential wound healing
dots.
from garlic. activity.
 07
Action Plan
 Action plan 0.24mg/ml
0.48mg/ml
0.6mg/ml
1. Dissolved in
Calibration curve distilled water
0.72mg/ml
0.84mg/ml
of ALA
0.96mg/ml
1.08mg/ml

0.24mg/ml
0.48mg/ml
0.6mg/ml
2. Dissolved in 0.72mg/ml
phosphate buffer 0.84mg/ml
(pH= 7.4) 0.96mg/ml
1.08mg/ml
 Action plan
1. UV Spectrum of alpha lipoic acid in water
Calibration curve
of ALA

1
Λ max = 334 nm
 Action plan
1. Calibration curve of alpha lipoic acid in water
Calibration curve
of ALA Distilled Water
λ = 334 nm
0.6
f(x) = 0.595218579234973 x − 0.0896393442622951
1 0.5
R² = 0.999963311490633
0.4

0.3

0.2

0.1

0
0 0.2 0.4 0.6 0.8 1 1.2
 Action plan
2. UV Spectrum of alpha lipoic acid in phosphate
Calibration curve buffer
of ALA

1
Λ max = 334 nm
 Action plan
2. Calibration curve of alpha lipoic acid in
Calibration curve phosphate buffer
of ALA
Phosphate Buffer Saline
pH= 7.4
0.8
1 0.7
f(x) = 0.636031420765028 x − 0.00861065573770503
0.6
R² = 0.999656492151056
0.5
0.4
0.3
0.2
0.1
0
0 0.2 0.4 0.6 0.8 1 1.2
 Action plan
Calibration curve
of ALA

Selection of
appropriate
preparation method
 Action plan

Selection of
appropriate
preparation method
 Action plan
In-vitro
Calibration curve Synthesis of characterization of the
of ALA garlic-derived developed system
carbon dots.

1 3 5

2 4

Selection of Loading drug on the

appropriate highly fluorescent
preparation method CDs
 Action plan
In-vitro Entrapment
characterization of the efficiency &
Transmission Loading
Electron developed system capacity
Microscopy

Drug
Release

X-Ray Fourier
Powder Transform
Zeta Potential Infrared
Diffraction
Spectroscop
y
 Action plan
In-vitro
Calibration curve Synthesis of characterization of the
of ALA garlic-derived developed system
carbon dots.

1 3 5

2 4 6

Selection of Loading drug on the Ex vivo evaluation

appropriate highly fluorescent
preparation method CDs
 References
1. Ejaz S, Chekarova I, Cho JW, Lee SY, Ashraf S, Lim CW. Effect of aged garlic
extract on wound healing: a new frontier in wound management. Drug Chem Toxicol.
2009;32(3):191-203. doi: 10.1080/01480540902862236. PMID: 19538015.

2. Külkamp-Guerreiro IC, Souza MN, Bianchin MD, Isoppo M, Freitas JS, Alves JA,
Piovezan AP, Pohlmann AR, Guterres SS. Evaluation of lipoic acid topical
application on rats skin wound healing. Acta Cir Bras. 2013 Oct;28(10):708-15. doi:
10.1590/s0102-86502013001000004. PMID: 24114299.

3. Hamide Ehtesabi and Reyhaneh Nasri 2021 Adv. Nat. Sci: Nanosci.
Nanotechnol. 12 025006DOI 10.1088/2043-6262/abffc9

4. Hu, Y., Lin, L., Li, J. et al. P,N Codoped carbon dots as an efficient “off-on”
fluorescent probe for lipoic acid detection and its cellular dual-color imaging. Anal
Bioanal Chem 411, 3603–3612 (2019).

5. Mehta VN, Chettiar SS, Bhamore JR, Kailasa SK, Patel RM. Green Synthetic
Approach for Synthesis of Fluorescent Carbon Dots for Lisinopril Drug Delivery
System and their Confirmations in the Cells. J Fluoresc. 2017 Jan;27(1):111-124. doi:
10.1007/s10895-016-1939-4. Epub 2016 Sep 28. PMID: 27679993.
 Faculty of
Pharmacy