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  • GC MS

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    Indah Budiapsari
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    1. Dr. Dhia Falih Al-Fekaiki of the University of Basrah provides instructions for analyzing samples using gas chromatography-mass spectrometry (GC-MS). 2. The document outlines the parameters for sample injection into the GC autosampler, inlet, carrier gas, oven temperature program, column type, and mass spectrometer settings. 3. The recommended method involves injecting a 0.2 μL sample into a split inlet at 200°C, separating components on a HP-5MS column using a temperature ramp program, and detecting ions from 20-400 amu on the mass spectrometer operated in scan mode.

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    GC-MS

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    1. Dr. Dhia Falih Al-Fekaiki of the University of Basrah provides instructions for analyzing samples using gas chromatography-mass spectrometry (GC-MS). 2. The document outlines the parameters for sample injection into the GC autosampler, inlet, carrier gas, oven temperature program, column type, and mass spectrometer settings. 3. The recommended method involves injecting a 0.2 μL sample into a split inlet at 200°C, separating components on a HP-5MS column using a temperature ramp program, and detecting ions from 20-400 amu on the mass spectrometer operated in scan mode.

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    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    6 views20 pages

    GC MS

    Uploaded by

    Indah Budiapsari
    1. Dr. Dhia Falih Al-Fekaiki of the University of Basrah provides instructions for analyzing samples using gas chromatography-mass spectrometry (GC-MS). 2. The document outlines the parameters for sample injection into the GC autosampler, inlet, carrier gas, oven temperature program, column type, and mass spectrometer settings. 3. The recommended method involves injecting a 0.2 μL sample into a split inlet at 200°C, separating components on a HP-5MS column using a temperature ramp program, and detecting ions from 20-400 amu on the mass spectrometer operated in scan mode.

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    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    of 20

    Interpretation

    of GC MS Results
    Dr. Dhia Falih Al-Fekaiki
    Expert of GC MS
    University of Basrah
    alfekaiki@yahoo.com
    Iraq- Basrah
    2017
    [AOC-20i+s]
    1

    2 [GC-2010]

    [GC Program]
    3

    [MS-2010]
    4
    AOC-20i+s 1
    GC and GCMS Autosampler System
    GC -2010 2
    MS-2010 3
    GC Program

    300C
    hold 3Min
    1 0C
    a se
    re
    Inc
    i n
    m
    ch1
    Ea
    180C
    hold 1Min
    1 5C
    ase
    e
    cr
    In
    in
    1m
    40C ch
    Ea

    hold 3Min
    Target

    Line#:16

    Hit 1
    Formlibrary
    NIST28

    Hit 2
    Formlibrary
    NIST28

    Hit 3
    Formlibrary
    NIST28
    Mean of peak
    number
    Target
    The compute 0f
    our samples

    Hit 1
    Formlibrary
    NIST28

    The computes
    which suggest Hit 2
    from NIST Formlibrary
    NIST28
    Library

    Hit 3
    Formlibrary
    NIST28
    Synonyms:
    1.2-Furancarboxaldehyde
    2.2-Furaldehyde
    Hit#:1 Entry:6349 Library:NIST08.LIB 3.α-Furole
    4.Artificial ant oil
    SI:96 Formula:C6H6O3 CAS:67-47-0 MolWeight:126 5.Fural
    RetIndex:1163 6.Furaldehyde
    7.Furale
    8.Furancarbonal
    9.Furfuraldehyde
    10.Furfurole
    11.Furfurylaldehyde
    12.Furole
    13.Pyromucic aldehyde
    14.2-Formylfuran
    15.2-Furanaldehyde
    16.2-Furancarbonal
    17.2-Furfural
    18.2-Furfuraldehyde
    19.2-Furylaldehyde
    20.Furol
    21.2-Furylmethanal
    22.Artificial oil of ants
    23.Furfurale
    24.Furfurol
    25.NCI-C56177
    26.2-Furil-metanale
    27.2-Furankarbaldehyd
    28.Rcra waste number U125
    29.2-Furylcarboxaldehyde
    30.Qo furfural
    31.Furan-2-aldehyde
    32.Furan-2-carbaldehyde
    33.NSC 8841
    5.3.2.1 Injection – Using the autosampler with a 10 μL syringe injection, perform three solvent washes from each wash bottle. Inject a 0.2
    μL sample with a fast plunger speed, no sample washes, and 3 sample pumps.

    5.3.2.2 Inlet – Run in split mode at 200 °C with a 100:1 split ratio.

    5.3.2.3 Carrier gas – Helium.

    5.3.2.4 Oven – Run a temperature program starting at 50.0 °C for 2.50 minutes. Then ramp at 10.0 °C per minute until 300 °C is obtained.
    Hold at 300 °C for 5.00 minutes.

    5.3.2.5 Column – Use a (5 %-Phenyl)-methylpolysiloxane (such as a HP-5MS or DB-5MS) column which is 0.25 mm in diameter that is
    approximately 30 m long with a 0.25 μm film thickness. The column shall be kept at a constant flow of 0.6 mL/min.

    5.3.2.6 Mass spectrometer – The transfer line temperature shall be set at 300 °C. The MS Quad temperature is set at 150 °C and the MS
    source is set at 230 °C. The MS shall be run in scan mode using the settings from the atune.u file with an electron multiplier offset of 200
    eV. The mass scan range is 20.0 amu to 400.0 amu with a threshold abundance of 150. The mass spectrometer shall have a 2.50 minute
    solvent de
    Thank you
    Dr . Dhia alfekaiki

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