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  • Blood Collection and Technique

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    Capillary blood collection involves puncturing the skin to obtain a small blood sample, usually from the finger or earlobe. Venous blood collection requires puncturing a vein, such as in the arm, to obtain a larger blood sample. The key steps for venous collection are selecting a vein, inserting the needle, releasing the tourniquet as blood fills the sytube, and applying pressure to stop bleeding. Venous collection provides more blood but is more technically difficult, while capillary collection only yields a small sample.

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    Capillary blood collection involves puncturing the skin to obtain a small blood sample, usually from the finger or earlobe. Venous blood collection requires puncturing a vein, such as in the arm, to obtain a larger blood sample. The key steps for venous collection are selecting a vein, inserting the needle, releasing the tourniquet as blood fills the sytube, and applying pressure to stop bleeding. Venous collection provides more blood but is more technically difficult, while capillary collection only yields a small sample.

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    3 views7 pages

    Blood Collection and Technique

    Uploaded by

    favourovuru
    Capillary blood collection involves puncturing the skin to obtain a small blood sample, usually from the finger or earlobe. Venous blood collection requires puncturing a vein, such as in the arm, to obtain a larger blood sample. The key steps for venous collection are selecting a vein, inserting the needle, releasing the tourniquet as blood fills the sytube, and applying pressure to stop bleeding. Venous collection provides more blood but is more technically difficult, while capillary collection only yields a small sample.

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    of 7

    Blood collection and

    technique
    Blood collection = is the process of taking blood sample from patients or subject.
    This process involved two method
    Capillary blood collection
    Venous blood collection
    • Capillary blood collection/puncture = is frequently use when small
    quantities of blood is required or needed, for example HB,PCV, GLCOUSE
    TEST, WBC/RBC, OR BLOOD SMEAR Preparation.
    • Site to be puncture
    • Adult = the ring finger or middle finger
    • Children = the ear lobe
    • Infant = big toe or kneel
    • Apparatus
    • Cotton wool
    • 70% alcohol
    • Lancet
    Techniques
    • Rub site vigorously with cotton moistened with alcohol.
    • Allow the skin to dry, make a puncture 2-3 mm deep with the lancet.
    • Stop the blood flow by applying slight pressure with cotton wool at the
    site.
    • Advantage of capillary blood collection/puncture .
    • It is obtained with ease.
    • It is pure no anti-coagulant is added.
    • Disadvantages of capillary blood collection /puncture.
    • Only small amount of blood can be obtained.
    • Platelet count can not be performed.
    • VENOUS BLOOD COLLECTION /PUNCTURE= Venous blood is the
    withdrawing of blood sample from the vein, and is used for most test that
    require larger quantities of blood, plasma, or serum.
    • SITE TO BE PUNCTURE = the three main veins of the fore arm, ante-cubital
    fossa of the arm
    • Apparatus
    • Syringe and needle
    • Tourniquet
    • Cotton wool
    • 70% alcohol sample bottle (EDTA)
    PROCEDURE
    • Assemble the syringe/needle.
    • Allow the subject to sit comfortable.
    • Prepare the arm by swabbing with cotton moistened with 70% alcohol.
    • Apply a tourniquet 6-7m above the elbow.
    • Select a vein.
    • Penetrate the skin first and then the vein, tourniquet should be released
    the moment blood start entering the syringe.
    • Apply a ball of cotton wool to the puncture site and gently withdraw the
    needle.
    • Cover the needle with it’s cap remove it from the nozzle of the syringe
    then empty the blood into a sample bottle.
    • Re-inspect the venipuncture site .
    Advantage of venous blood
    collection/puncture
    • It provide sufficient amount of blood
    • May be frozen for future use
    • it has plasma and serum

    • DISADVANTAGE OF VENOUS BLOOD COLLECTION/PUNCTURE


    • It is technically difficult in children
    • It can lead to hematoma
    Assignment
    • 1) what is the difference between capillary and venous blood
    • 2)state the meaning of the following and their function
    • EDTA
    • TRI-SODUM
    • HEPARIN
    • DOUBLE OXALATE
    • State the different between plasma and serum

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