a ttB 2 H I F 1 a -S to p -s H I F 1 a -S to p -a

P s tI ( 8 3 0 5 )

p D O N O R 2 2 3 -H I F 1 a F L -5 1 5 8 b p -s
E c oR I ( 7 4 5 2 )

H IF 1 a
E c oR I ( 6 8 4 8 ) H i nd III (6 3 5 9 )

V5 TK p A TK p o ly a d e n y la tio n s ig n a l f1 o r i S V 40ori
N c oI ( 1 0 6 1 ) X m a I (1 1 7 5 ) A v a I (1 1 7 5 )

P r im e r a in P r im e r s E n d in P r im e r a E n d bH LH ?
A v a I (5 9 2 9 )

i n P r i m e r s S ta r t i n P r i m e r a S ta r t a ttB 1
A v a I (5 8 2 8 )

p E x p - C F P - h H IF 1 a
8 353 bp

S m a I (1 1 7 7 )

EM7
N c oI ( 1 2 8 1 )

B la s tic id in S V 40 pA
A p a L I (2 66 4 )

CFP
N c oI ( 5 1 0 7 ) A v aI (5 1 0 0 ) B a m H I (5 0 8 0 ) N c oI ( 4 7 7 6 )

pU C ori A m p ic illin
A p a L I (3 91 0 ) A p a L I (4 1 9 8 )

CMV

A m p p r o m o to r (b la )

Seq. of the HIF-1a 1 TTGTACAAAG TGGTTGATAA CGGGAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGCGTACCG GTTAGTAATG AGTTTAAACG GGGGAGGCTA 101 ACTGAAACAC GGAAGGAGAC AATACCGGAA GGAACCCGCG CTATGACGGC AATAAAAAGA CAGAATAAAA CGCACGGGTG TTGGGTCGTT TGTTCATAAA 201 CGCGGGGTTC GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC GCGTTTCTTC CTTTTCCCCA CCCCACCCCC 301 CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG CCAACGTCGG GGCGGCAGGC CCTGCCATAG CAGATCTGCG CAGCTGGGGC TCTAGGGGGT ATCCCCACGC 401 GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT

CGCTTTCTTC 501 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGCATCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 601 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT 701 CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 801 ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT 901 GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC 1001 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG 1101 CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG 1201 ATCTGATCAG CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC CATGGCCAAG CCTTTGTCTC 1301 AAGAAGAATC CACCCTCATT GAAAGAGCAA CGGCTACAAT CAACAGCATC CCCATCTCTG AAGACTACAG CGTCGCCAGC GCAGCTCTCT CTAGCGACGG 1401 CCGCATCTTC ACTGGTGTCA ATGTATATCA TTTTACTGGG GGACCTTGTG CAGAACTCGT GGTGCTGGGC ACTGCTGCTG CTGCGGCAGC TGGCAACCTG 1501 ACTTGTATCG TCGCGATCGG AAATGAGAAC AGGGGCATCT TGAGCCCCTG CGGACGGTGC CGACAGGTGC TTCTCGATCT GCATCCTGGG ATCAAAGCCA 1601 TAGTGAAGGA CAGTGATGGA CAGCCGACGG CAGTTGGGAT TCGTGAATTG CTGCCCTCTG GTTATGTGTG GGAGGGCTAA GCACTTCGTG GCCGAGGAGC 1701 AGGACTGACA CGTGCTACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA 1801 GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT 1901 AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 2001 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA

2101 ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG 2201 GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 2301 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 2401 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG 2501 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 2601 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 2701 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC 2801 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 2901 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 3001 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG 3101 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT 3201 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 3301 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC 3401 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT 3501 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT

3601 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA 3701 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG 3801 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 3901 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA 4001 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT 4101 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCGACGG ATCGGGAGAT CTCCCGATCC CCTATGGTGC 4201 ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATCTGCT CCCTGCTTGT GTGTTGGAGG TCGCTGAGTA GTGCGCGAGC AAAATTTAAG 4301 CTACAACAAG GCAAGGCTTG ACCGACAATT GCATGAAGAA TCTGCTTAGG GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG 4401 ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC 4501 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG 4601 TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG 4701 GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC 4801 ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT 4901 TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA 5001 GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC GGGACCGATC CAGCCTCCGG ACTCTAGAGG ATCCCTACCG GTGATATCCT

5101 CGAGACCATG GTGAGCAAGG GCGAGGAGCT GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG CAGCGTGTCC 5201 GGCGAGGGCG AGGGCGATGC CACCTACGGC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GTGACCACCC 5301 TGACCTGGGG CGTGCAGTGC TTCAGCCGCT CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA AGGAGCGCAC 5401 CATCTTCTTC AAGGACGACG GCAACTACAA GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG CATCGACTTC 5501 AAGGAGGACG GCAACATCCT GGGGCACAAG ACTACATCAG CCACAACGTC TATATCACCG CCGACAAGCA ATCAAGGCCA 5601 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC TGCTGCCCGA 5701 CAACCACTAC CTGAGCACCC AGTCCGCCCT CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG CGGGATCACT 5801 CTCGGCATGG ACGAGCTGTA CAAGGGCTCG CAAGTTTGTA CAAAAAAGTT GGCATGGAGG GCGCCGGCGG AAGAAAAAGA 5901 TAAGTTCTGA ACGTCGAAAA GAAAAGTCTC CAGATCTCGG CGAAGTAAAG AATCTGAAGT TTTTTATGAG AGTTGCCACT 6001 TCCACATAAT GTGAGTTCGC ATCTTGATAA ATGAGGCTTA CCATCAGCTA TTTGCGTGTG AGGAAACTTC TGATTTGGAT 6101 ATTGAAGATG ACATGAAAGC ACAGATGAAT TGAAAGCCTT GGATGGTTTT GTTATGGTTC TCACAGATGA ATTTACATTT 6201 CTGATAATGT GAACAAATAC ATGGGATTAA ACTAACTGGA CACAGTGTGT TTGATTTTAC TCATCCATGT AAATGAGAGA 6301 AATGCTTACA CACAGAAATG GCCTTGTGAA GAACAAAACA CACAGCGAAG CTTTTTTCTC AGAATGAAGT TAGCCGAGGA 6401 AGAACTATGA ACATAAAGTC TGCAACATGG ACTGCACAGG CCACATTCAC GTATATGATA CCAACAGTAA TGTGGGTATA 6501 AGAAACCACC TATGACCTGC TTGGTGCTGA CATTCCTCAC CCATCAAATA TTGAAATTCC TTTAGATAGC

GTTCACCGGG GCCACAAGTT AAGCTGACCC GCCCACCCTC ACCCCGACCA GGCTACGTCC GACCCGCGCC AGCTGAAGGG CTGGAGTACA GAAGAACGGC GCAGCGTGCA GGCCCCGTGC GAGCAAAGAC TGACCGCCGC AGCCCATCAA CGCGAACGAC GAGATGCAGC CTTGCTCATC GGCCTCTGTG TGGATGCTGG TGCTTTTATT TGGTGACATG CTCAGTTTGA GACCATGAGG AAAGGGTAAA GTACCCTAAC AAGGTATTGC CCAACCTCAG TTTGTGAACC AAGACTTTCC

TCAGTCGACA 6601 CAGCCTGGAT ATGAAATTTT CTTATTGTGA ACCGAATTGA TGGGATATGA GCCAGAAGAA CTTTTAGGCC TGAATATTAT 6701 CATGCTTTGG ACTCTGATCA TCTGACCAAA ATATGTTTAC TAAAGGACAA GTCACCACAG GACAGTACAG AAAAGAGGTG 6801 GATATGTCTG GGTTGAAACT CAAGCAACTG CACCAAGAAT TCTCAACCAC AGTGCATTGT ATGTGTGAAT GTGGTATTAT 6901 TCAGCACGAC TTGATTTTCT CCCTTCAACA GTCCTTAAAC CGGTTGAATC TTCAGATATG AAAATGACTC CAAAGTTGAA 7001 TCAGAAGATA CAAGTAGCCT CTTTGACAAA AACCTGATGC TTTAACTTTG CTGGCCCCAG CCGCTGGAGA TCTTTAGATT 7101 TTGGCAGCAA CGACACAGAA ACTGATGACC GGAAGTACCA TTATATAATG ATGTAATGCT CCCCTCACCC TACAGAATAT 7201 AAATTTGGCA ATGTCTCCAT TACCCACCGC AAGCCACTTC GAAGTAGTGC TGACCCTGCA CTCAATCAAG AAAATTAGAA 7301 CCAAATCCAG AGTCACTGGA ACTTTCTTTT AGATTCAGGA TCAGACACCT AGTCCTTCCG ATGGAAGCAC TCACCTGAGC 7401 CTAATAGTCC CAGTGAATAT TGTTTTTATG TATGGTCAAT GAATTCAAGT TGGAATTGGT AGAAAAACTT ACACAGAAGC 7501 AAAGAACCCA TTTTCTACTC AGGACACAGA GAGATGTTAG CTCCCTATAT CCCAATGGAT GATGACTTCC CTTCGATCAG 7601 TTGTCACCAT TAGAAAGCAG TTCCGCAAGC CAAGTCCTCA AAGCACAGTT ACAGTATTCC AGCAGACTCA CCTACTGCTA 7701 ATGCCACCAC TACCACTGCC ACCACTGATG AGTGACAAAA GACCGTATGG AAGACATTAA AATATTGATT CTCCTACCCA 7801 CATACATAAA GAAACTACTA GTGCCACATC AGAGATACTC AAAGTCGGAC AGCCTCACCA AACAGAGCAG CATAGAACAG 7901 ACAGAAAAAT CTCATCCAAG AAGCCCTAAC TCGCTTTGAG TCAAAGAACT ACAGTTCCTG AGGAAGAACT ATACTAGCTT 8001 TGCAGAATGC TCAGAGAAAG CGAAAAATGG

TGAAAGAATT GCTCAATTTA ACTCATCATG GATGCTTGCC TCATATATAA TACGTTGTGA AACAGAATGT AGCTATTCAC CTTAAGAAGG CACAATCATA AGCAACTTGA AACGAAAAAT TGAAACGCCA AAGTTGCATT ACCATGCCCC TAGACAAAGT TGGATAGTGA TTTGCTGAAG TTTAGACTTG AGTTACGTTC CCTGAAAGCG AATACAAGAA AATTAAAAAC GCATCTCCAT ATCACCATAT GAAAAGGAGT GTGTTATCTG AAATCCAAAG AACATGATGG

TTCACTTTTT CAAGCAGTAG GAATTGGAAC ATTATTACAG CAGCCAGACG ATCATGCAGC 8101 TACTACATCA CTTTCTTGGA AACGTGTAAA AGGATGCAAA TCTAGTGAAC AGAATGGAAT GGAGCAAAAG ACAATTATTT TAATACCCTC TGATTTAGCA 8201 TGTAGACTGC TGGGGCAATC AATGGATGAA AGTGGATTAC CACAGCTGAC CAGTTATGAT TGTGAAGTTA ATGCTCCTAT ACAAGGCAGC AGAAACCTAC 8301 TGCAGGGTGA AGAATTACTC AGAGCTTTGG ATCAAGTTAA CTGACCAACT TTC

a ttB 2
E c oR I ( 8 1 9 6 ) P s tI ( 8 0 0 3 )

P r im e r 4 r e v P r i m e r 4 fo r
P s tI ( 7 7 2 1 )

P r im e r 3 r e v P r i m e r 3 fo r seq com pl okay

E c oR I ( 7 0 5 9 )

hA R N T P r im e r 2 r e v P r i m e r 2 fo r s e q d ir e c t o k a y
A p a L I (6 5 0 4 )

V5 TK p A TK p o ly a d e n yla tio n s ig n a l f1 o r i S V 4 0 o ri
N c oI ( 1 0 6 1 ) X m a I (1 1 7 5 ) A v aI (1 1 7 5 )

P r im e r 1 r e v P r i m e r 1 fo r
E c oR I ( 5 9 5 3 ) N c oI ( 5 8 6 6 )

K ozak consensus sequence a ttB 1

A v aI (5 8 2 8 )

pExp-YFP-hAR N T
8 247 bp

S m a I (1 1 7 7 )

EM7
N c oI ( 1 2 8 1 )

E G F P -C S e q P r im e r YFP
N c oI ( 5 1 0 7 ) A v aI (5 1 0 0 ) B a m H I (5 0 8 0 ) N c oI ( 4 7 7 6 )

B la s tic id in S V 40 pA
A p a L I (2 66 4 )

pU C ori A m p ic illin
A p a L I (3 91 0 ) A p a L I (4 1 9 8 )

CMV

A m p p r o m o to r (b l a )

1 TTGTACAAAG TGGTTGATAA CGGGAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGCGTACCG GTTAGTAATG AGTTTAAACG GGGGAGGCTA 101 ACTGAAACAC GGAAGGAGAC AATACCGGAA GGAACCCGCG CTATGACGGC AATAAAAAGA CAGAATAAAA CGCACGGGTG TTGGGTCGTT TGTTCATAAA 201 CGCGGGGTTC GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC GCGTTTCTTC CTTTTCCCCA CCCCACCCCC 301 CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG CCAACGTCGG GGCGGCAGGC CCTGCCATAG CAGATCTGCG CAGCTGGGGC TCTAGGGGGT ATCCCCACGC

401 GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 501 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGCATCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 601 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT 701 CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 801 ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT 901 GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC 1001 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG 1101 CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG 1201 ATCTGATCAG CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA GGAACTAAAC CATGGCCAAG CCTTTGTCTC 1301 AAGAAGAATC CACCCTCATT GAAAGAGCAA CGGCTACAAT CAACAGCATC CCCATCTCTG AAGACTACAG CGTCGCCAGC GCAGCTCTCT CTAGCGACGG 1401 CCGCATCTTC ACTGGTGTCA ATGTATATCA TTTTACTGGG GGACCTTGTG CAGAACTCGT GGTGCTGGGC ACTGCTGCTG CTGCGGCAGC TGGCAACCTG 1501 ACTTGTATCG TCGCGATCGG AAATGAGAAC AGGGGCATCT TGAGCCCCTG CGGACGGTGC CGACAGGTGC TTCTCGATCT GCATCCTGGG ATCAAAGCCA 1601 TAGTGAAGGA CAGTGATGGA CAGCCGACGG CAGTTGGGAT TCGTGAATTG CTGCCCTCTG GTTATGTGTG GGAGGGCTAA GCACTTCGTG GCCGAGGAGC 1701 AGGACTGACA CGTGCTACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA 1801 GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT 1901 AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 2001 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC

GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA 2101 ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG 2201 GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 2301 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 2401 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG 2501 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 2601 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 2701 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC 2801 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 2901 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 3001 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG 3101 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT 3201 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 3301 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC 3401 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT 3501 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC

CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT 3601 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA 3701 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG 3801 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 3901 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA 4001 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT 4101 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCGACGG ATCGGGAGAT CTCCCGATCC CCTATGGTGC 4201 ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATCTGCT CCCTGCTTGT GTGTTGGAGG TCGCTGAGTA GTGCGCGAGC AAAATTTAAG 4301 CTACAACAAG GCAAGGCTTG ACCGACAATT GCATGAAGAA TCTGCTTAGG GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG 4401 ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC 4501 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG 4601 TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG 4701 GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC 4801 ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT 4901 TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA 5001 GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT

AGAAGACACC GGGACCGATC CAGCCTCCGG ACTCTAGAGG GTGATATCCT 5101 CGAGACCATG GTGAGCAAGG GCGAGGAGCT GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG CAGCGTGTCC 5201 GGCGAGGGCG AGGGCGATGC CACCTACGGC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GTGACCACCT 5301 TCGGCTACGG CGTGCAGTGC TTCGCCCGCT CATGCGCCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA AGGAGCGCAC 5401 CATCTTCTTC AAGGACGACG GCAACTACAA GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG CATCGACTTC 5501 AAGGAGGACG GCAACATCCT GGGGCACAAG ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA ATCAAGGTGA 5601 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC TGCTGCCCGA 5701 CAACCACTAC CTGAGCTACC AGTCCGCCCT CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG CGGGATCACT 5801 CTCGGCATGG ACGAGCTGTA CAAGGGCTCG CAAGTTTGTA CAAAAAAGCA GGCACCATGG CGGCGACTAC GAAATGACAT 5901 CAGATGTACC ATCACTGGGT CCAGCCATTG CTCTGGACCT GGAATTCAAG GTGGAGGAGC CATTGTCCAG AGCGGCGACC 6001 AGGGCTGGAT TTTGATGATG ATGGAGAAGG TTTTTGAGGT GTGATGATGA TCAGATGTCT AACGATAAGG CAGGTCGGAT 6101 GATGAGCAGA GCTCTGCGGA TAAAGAGAGA AAAATCACAG TGAAATTGAA CGGCGGCGAC GGAACAAGAT ATCACAGAAC 6201 TGTCAGATAT GGTACCCACC TGTAGTGCCC ACCAGACAAG CTAACCATCT TACGCATGGC AGTTTCTCAC TGCGGGGAAC 6301 TGGCAACACA TCCACTGATG GCTCCTATAA CTCACTGATC AGGAACTGAA ACATTTGATC TTGGAGGCAG TCTGTTTATT 6401 GTCTCATGTG AGACAGGCAG GGTGGTGTAT CCGTGACTCC TGTTTTGAAC CAGCCACAGT CTGAATGGTT CTCTATGATC

ATCCCTACCG GTTCACCGGG GCCACAAGTT AAGCTGACCC GCCCACCCTC ACCCCGACCA GGCTACGTCC GACCCGCGCC AGCTGAAGGG CTGGAGTACA GAAGAACGGC GCAGCGTGCA GGCCCCGTGC GAGCAAAGAC TGACCGCCGC AGCCCATCAA TGCCAACCCC CCTCTGGAAA AGGGCTATTA GAACAGTAAA AGCGGTTTGC CTTGCCAGGG GACAGCCTAC TGGCTCGAAA ATGAAGTCCT GCCGTCTTTC CAGATGGCTT GTGTCTGACT TGGCAGCACA

6501 AGGTGCACCC AGATGATGTG GATAAACTTC TTCCACTTCA GAAAATGCCC TGACAGGGCG TATCCTGGAT GAACAGTGAA 6601 AAAGGAAGGT CAGCAGTCTT CCATGAGAAT TCAAGGAGAT CGTTTATTTG CCGAATGAGG TGTGGCAGTA CCCAGTTTCT 6701 GTGAATAGGC TGAGCTTTGT GAGGAACAGA GACTTGGCTC TGTAAAGGAT GGGGAACCTC ACTTCGTGGT ACAGGCTACA 6801 TCAAGGCCTG GCCCCCAGCA GGTGTTTCCC TGACCCAGAG GCTGGCCAGG GAAGCAAGTT TTGCCTAGTG GATTGCAGGT 6901 AACTAGTTCT CCCAACTGTA CAGACATGAG CAACCAACAG AGTTCATCTC CCGACACAAC ATTGAGGGTA TGTGGATCAC 7001 CGCTGTGTGG CTACTGTTGG CTACCAGCCA TAGGAAAGAA TATTGTAGAA TTCTGTCATC CTGAAGACCA AGAGACAGCT 7101 TCCAACAGGT AGTGAAATTA AAAGGCCAAG CATGTTCCGG TTCCGGTCTA AGAACCAAGA ATGGCTCTGG GCTCCTTTAC 7201 TTTCCAGAAC CCTTACTCAG ATGAAATTGA TGTACCAACA CCAATGTGAA GAACTCTAGC CAAGAACCAC CTCCAACACA 7301 ATCCAGAGGC CACAACTAGG TCCCACAGCT TGGAGATGGG CTCAGGACAG CTGGCACCCA GGCAGCAGCA GAATTGGACA 7401 TGGTACCAGG AAGAGATGGA CTGGCCAGCT CCAGGTGGTT CAGCCTGTGA CAACCACAGG ACCAGAACAC TTGAGAAGTC 7501 AGATGGTTTA TTTGCCCAGG ATAGAGATCC GAAATCTATC ACAACATCAA TGCGGATCAG AGTAAAGGCA CACTGTCCCT 7601 GCCACCCAAC AGCTATTCTC CCAGGGCAAC CTACCCCCCG GCCGGCAGAG AATTTCAGGA ATAGTGGCCT GTAACCATTG 7701 TCCAGCCATC AGCTTCTGCA GGACAGATGT TTCCCGCCAC TCCAACCCCA CCCAAGGAGC AACCCCAACT CTACCCGCTC 7801 AGGCTTTTCT GCCCAGCAGG TGGCTACCCA AAGACTCGTA CTTCCCAGTT TGGTGTGGGC AGCTTTCAGA CTTCAGCTCC 7901 ATGTCCCTCC CTGGTGCCCC AACTGCATCG CTGCCTACCC TAGTCTCACC AATCGTGGAT CTAACTTTGC

GTGAGCAGCT CTAAAGACTG GTGTATGGGC GCTCTGTGGA TGCAGGAATG GGTCCACTGC TCCCAGATGA GCCATTGGCA TAATGTTTGT TCTTCACTTT CAGGAACTCT GCAGCTTCTA TGCTGTCTGT ATGAGAACCA GTACATCATC GGCCTACACT AATTTACCCC ACAGCAAACA ACAATCATTC AGCAAGCCCC AAGATTTTCA TCTCCTCCAG ACATTCCCTC AGCCCCTCCT TGGCCCAGAT TGGACCCCTA GGCTACTGCT CTCCATCCTC CCTGGTGCTG TCCTGAGACT

GGACAGACTG 8001 CAGGACAATT CCAGACACGG ACAGCAGAGG GTGTGGGTGT CTGGCCACAG TGGCAGGGCC AGCAGCCTCA TCATCGTTCA AGTTCTAGTG AGCAACATGT 8101 TCAACAACCG CCAGCACAGC AACCTGGCCA GCCTGAGGTC TTCCAGGAGA TGCTGTCCAT GCTGGGAGAT CAGAGCAACA GCTACAACAA TGAAGAATTC 8201 CCTGATCTAA CTATGTTTCC CCCCTTTTCA GAATAGACCC AGCTTTC