Appendix 1

FORM 4 : CHAPTER 6 - NUTRITION

PLANNING SKILLand REPORT

Aim/
Objective
: To determine the vitamin C content in different fruit juice E1 PI1-1
Problem
statement
: oes lime juice contain more vitamin C than !inea!!le
juice"
E1 PI 1-#
$%!othesis : &ime juice contain more vitamin C than !inea!!le juice' E1 PI 1-(
)ani!ulated
*ariable
: T%!es of fruit juice
+es!ondin,
*ariable
: *olume of fruit juice needed to decolori-e the 1 ml

CPIP solution / . of vitamin C
E1 PI 1-/
0i1ed *ariable : *olume of CPIP solution
)aterial : 2'1. ascorbic acid solution3 CPIP solution3 freshl%
!re!ared fruit juice and !inea!!le juice
A!!aratus : 4!ecimen tubes3 a s%rin,e 51 ml63 s%rin,es 57ml6 8ith
needles3 bea9ers 572ml63 ,au-e cloth3 9nife and mortar'
E1 PI 1-7
Techni:ue : To record the volume of fruit juice needed to decolori-e
the 1 ml

CPIP usin, a scaled s%rin,e'
E1 PI 1-;
Procedure 1' Three s!ecimen tubes are labeled as ascorbic acid3 lime
juice and !inea!!le juice'
#' 1 ml of CPIP solution is !laced in each s!ecimen tube'
(' A s%rin,e is filled 8ith 7 ml of ascorbic acid solution'
/' The needle of the s%rin,e is immersed in the CPIP
solution'
7' The ascorbic solution is added dro! b% dro! to the CPIP
solution and the tube is sha9en slo8l%'
;' The amount of ascorbic acid solution use to decolori-e
the CPIP solution is recorded'
E1 PI 1-<
<' 4te!s # to ; are re!eated usin, lime and !inea!!le juices'
=' The !ercenta,e and concentration of vitamin C in the in
the fruit juices are calculated' usin, the follo8in,
>' The data collected is recorded in a table'
TEAC$E+?4 4I@AATB+E : CCCCCCC''
+esults
:
4olution Initial
*olume
5ml6
0inal
*olume
5ml6
*olume
used
5ml6
Percenta,e Of
*itamin C
5.6
Concentration
Of *itamin C
5m, ml6
Ascorbic
Acid
7'2 /'2 1'2 - -
&ime
juice 7'2 #'7 #'7
1/#'7 1
2'1D2'2/
2'/
Pinea!!le
juice
7'2 1'/ (';
1/('; 1
2'1D2'2(
2'(
E(PI 1
Cal!la"i#n:
Pe$en"a%e #& 'i"a(in C) *#l!(e #& +,1- a.#$/i aid .#l!"i#n 0 +,1
*#l!(e #& &$!i" 1!ie
C#nen"$a"i#n #& *i"a(in C ) *#l!(e #& +,1- a.#$/i aid .#l!"i#n
*#l!(e #& &$!i" 1!ie
5a6 Percenta,e of vitamin C in lime juice D 1/#'7 1 2'1D2'2/

Concentration of *itamin C In lime juice D 1/#'7 D 2'/ m, /ml
5b6 Percenta,e of vitamin C in !inea!!le juice D 1/('; 1 2'1D2'2(

Concentration of *itamin C in !inea!!le juice D 1/('; D 2'(m, /ml
E/ PI 1
iscussion : The ,reater the volume of fruit juice needed to decolori-e
1ml CPIP solution3 the lo8er the content of vitamin C
in the fruit juice
E/ PI #
The volume of lime juice used to decolori-e 1ml CPIP
solution is #'7 ml

8hereas the volume of !inea!!le juice
used is ('; ml'
E/ PI #
Conclusion : &ime juice contain more vitamin C than !inea!!le juice'
The h%!othesis is acce!ted
E/ PI(