"A"

Unk

"B"

Unk

"C"

NTC

"D"
A01

SYBR Unkn CA9

TI

27.17 27.17 0.000

B01

SYBR Unkn CA9

26.52 26.52 0.000

C01

SYBR NTC

CA9

27.86 27.86 0.000

Well Fluor Content

Target
Sample
Height
Begin Temperature End Temperature
B01

SYBR Unkn CA9

Target
Sample
Ctrl
Expression SEM
Mean C(t)

Melt Temperature Peak

81.00 0.03

75.50 86.00

Expression
C(t) SEM

Expression SEM

Corrected

CA9

N/A

N/A

N/A

26.52

0.00000

CA9

TI

N/A

N/A

N/A

27.17

0.00000

1 50.0 C for 10:00

2 95.0 C for 5:00
3 95.0 C for 0:10
4 56.0 C for 0:30
5 72.0 C for 0:30

+ Plate Read
6 GOTO 3, 39 more times
7 95.0 C for 1:00
8 55.0 C for 1:00
9 Melt Curve 55.0 to 95.0 C, increment 0.5 C,
0:10 + Plate Read
10 20.0 C for 0:30
END