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  • Primer

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    NM_008362 is from Mus musculus, but the organism for specificty checking is limited to Homo sapiens. Primer pairs are specific to input template as no other targets were found in selected database: GenBank refseq mRNA. Primer pair 1 sequence (5'->3') Forward primer Reverse primer Product length template strand Self complementarit ACTTGAGGAGGCAGTTTTCGT Plus CCGCTGATATGGGTCTTG Plus 336 primer pair

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    NM_008362 is from Mus musculus, but the organism for specificty checking is limited to Homo sapiens. Primer pairs are specific to input template as no other targets were found in selected database: GenBank refseq mRNA. Primer pair 1 sequence (5'->3') Forward primer Reverse primer Product length template strand Self complementarit ACTTGAGGAGGCAGTTTTCGT Plus CCGCTGATATGGGTCTTG Plus 336 primer pair

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    3 views3 pages

    Primer

    Uploaded by

    acezeeshan
    NM_008362 is from Mus musculus, but the organism for specificty checking is limited to Homo sapiens. Primer pairs are specific to input template as no other targets were found in selected database: GenBank refseq mRNA. Primer pair 1 sequence (5'->3') Forward primer Reverse primer Product length template strand Self complementarit ACTTGAGGAGGCAGTTTTCGT Plus CCGCTGATATGGGTCTTG Plus 336 primer pair

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    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
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    of 3

    Primer-BLAST Primer-Blast results

    NCBI/ Primer-BLAST : results: Job id=JSID_01_328653_130.14.22.21_9002 more...

    Warnings: The template sequence is from Mus musculus, but the organism for specificty checking is limited to Homo
    sapiens.

    Input PCR template NM_008362.2 Mus musculus interleukin 1 receptor, type I (Il1r1), transcript variant 1, mRNA Range 1 - 4739 Specificity of primers Primer pairs are specific to input template as no other targets were found in selected database: Refseq mRNA (Organism limited to Homo sapiens)

    Search Summary

    For better specificity checking, we have substituted the PCR template with the GenBank refseq record
    NM_008362.2 which is identical to your input template

    Graphical view of primer pairs


    NM_008362.2: 1..4.7K (4.7Kbp) Find on Sequence: +

    Genes - Exon Genes Primer pairs for NM_008362.2 Template

    Detailed primer reports


    Primer pair 1

    Sequence (5'->3') Forward primer Reverse primer Product length

    Template strand

    Length Start Stop Tm GC% 20 20 29 48

    Self complementarity

    TGTCATTCTTGTGCACGCCA Plus ACGTCAATCTCCAGCGACAG Minus 267

    60.82 50.00 6.00

    295 276 60.11 55.00 4.00

    Primer pair 2

    Sequence (5'->3') Forward primer Reverse primer Product length

    Template strand

    Length Start Stop Tm GC% 20 20 93

    Self complementarity

    TCCTGGGCTGACTTGAGGAG Plus ATATTGCTTCCCCCGGAACG Minus 762

    112 60.91 60.00 4.00

    854 835 60.18 55.00 5.00

    Primer pair 3

    Sequence (5'->3') Forward primer Reverse primer Product length

    Template strand

    Length Start Stop Tm GC% 21 20

    Self complementarit

    ACTTGAGGAGGCAGTTTTCGT Plus CCGCTGATATGGGGGTCTTG Minus 336

    103 123 59.86 47.62 3.00 438 419 60.25 60.00 4.00

    Primer pair 4

    Sequence (5'->3') Forward primer Reverse primer Product length

    Template strand

    Length Start Stop Tm GC% 21 20 82

    Self complementarit

    AAGTAATGCTGTCCTGGGCTG Plus GCAGAGGCACCATGAGACAA Minus 195

    102 60.34 52.38 3.00

    276 257 60.32 55.00 4.00

    Primer pair 5

    Sequence (5'->3') Forward primer Reverse primer Product length


    v

    Template strand

    Length Start Stop Tm GC% 21 20 43 63

    Self complementarit

    ACGCCAGCCCAGTAATCATTT Plus CAAGGTCTGAGAACTGGCCC Minus 963

    60.34 47.62 5.00

    1005 986 60.32 60.00 7.00

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