std curve

140
f(x) = 1.26x
IFN A B C Av Av-bl 120 R² = 1
0 5.3 5.7 5.6 5.53 0 Y=1.2603X - 1.4086 100
6.25 13.6 13.3 13.8 13.57 8.03 80
12.5 20.1 19.6 20.2 19.97 14.43
60
25 33.9 33.7 32.9 33.5 27.97
50 64.6 64.4 64.3 64.43 58.9 40
100 132.5 132.6 130.7 131.93 126.4 20
200 359 357.8 349.4 0
0
0 5.7 6.7 6.2 0 Y= 0.3583x - 2.7111
15.6 10 9 9.5 3.3 800
31.25 14.8 16.8 15.8 9.6 f(x) = 0.36x
700 R² = 1
62.5 27.5 27.5 21.3
600
125 48.4 48.6 48.5 42.3
250 91.7 91.7 91.7 85.5 500
500 179.1 178.5 178.8 172.6 400
1000 357.8 358.2 358 351.8 300
2000 718.5 727.7 723.1 716.9
200
100
0
sample 3 0 5

Pr
hour A B C Av Av-bl std dev %cv df [IFN]
'2h 3Pr' 13.1 10.7 11.5 11.77 5.57 1.22 10.39 2 23.1
'4h 3Pr' 4.9 6.5 6.4 5.93 -0.27 0.9 15.11 2 6.82
'6h 3Pr' 23.1 22.1 23 22.73 16.53 0.55 2.42 2 53.71
'10h 3Pr' 48.3 49.7 49.3 49.1 42.9 0.72 1.47 2 127.3
'14h 3Pr' 146.5 145.5 143.5 145.17 138.97 1.53 1.05 2 395.42
'18h 3Pr' 53.2 52.9 53.2 53.1 46.9 0.17 0.33 2 138.46

TC
hour A B C Av Av-bl std dev %cv df [IFN]
2 17.4 17.4 14.7 16.5 10.3 1.56 9.45 2 36.31
4 25.9 22.8 21.2 23.3 17.1 2.39 10.26 2 55.29
6 30.4 31.1 30.5 30.67 24.47 0.38 1.23 2 75.85
10 38.4 39.5 38.8 38.9 32.7 0.56 1.43 2 98.83
14 46.2 48.1 47.5 47.27 41.07 0.97 2.05 2 122.18
18 43.1 43.4 43.9 43.47 37.27 0.4 0.93 2 111.58

IB
hour A B C Av Av-bl std dev %cv df [IFN]
2 5.8 5.8 6.2 5.93 0.4 0.23 3.89 20 1.44
4 5.2 4.4 4.5 4.7 -0.83 0.44 9.27 20 0.46
6 9.6 9.8 10.2 9.87 4.33 0.31 3.1 20 4.56
14 12.8 13.2 13.4 13.13 7.6 0.31 2.33 20 7.15
18 11.3 12.1 11.7 11.7 6.17 0.4 3.42 20 6.01
sample 4
Pr
hour A B C Av Av-bl std dev %cv df [IFN]
2 13.9 14 14.1 14 7.8 0.1 0.71 2 29.34
4 12.1 11.2 10.9 11.4 5.2 0.62 5.48 2 22.08
6 22.2 22.3 22.5 22.33 16.13 0.15 0.68 2 52.59
10 83.7 83.6 81.9 83.07 76.87 1.01 1.22 2 222.1
14 219.2 222.2 220.9 220.77 214.57 1.5 0.68 2 606.41
18 107.9 104.3 104.9 105.7 99.5 1.93 1.82 2 285.27

TC
hour A B C Av Av-bl std dev %cv df [IFN]
2 15.3 14.9 14.8 15 8.8 0.26 1.76 2 32.13
4 21.9 22.2 21.8 21.97 15.77 0.21 0.95 2 51.57
6 24.5 25.1 25.7 25.1 18.9 0.6 2.39 2 60.32
10 42.3 41.5 41.8 41.87 35.67 0.4 0.97 2 107.11
14 69.1 72.5 71.5 71.03 64.83 1.75 2.46 2 188.51
18 57.4 56.2 56.8 56.8 50.6 0.6 1.06 2 148.79

IB
hour A B C Av Av-bl std dev %cv df [IFN]
2 6.3 5.3 5.8 5.8 0.27 0.5 8.62 20 1.33
4 5.7 6.2 5.3 5.73 0.2 0.45 7.86 20 1.28
6 9 9.6 9.3 3.77 0.42 4.56 20 4.11
14 20.2 20.1 20.15 14.62 0.07 0.35 20 12.72
18 15.5 15.1 15.3 15.3 9.77 0.2 1.31 20 8.87

sample 11
Pr
hour A B C Av Av-bl std dev %cv df [IFN]
2 13.7 13.8 13.4 13.63 7.43 0.21 1.53 2 28.31
4 12.5 12.8 12.7 12.67 6.47 0.15 1.21 2 25.61
6 7.7 9.1 9.6 8.8 2.6 0.98 11.19 2 14.82
10 45.5 46.6 45.4 45.83 39.63 0.67 1.45 2 118.18
14 52.9 52.1 52.4 52.47 46.27 0.4 0.77 2 136.69
18 48.9 48.3 46.9 48.03 41.83 1.03 2.14 2 124.32

TC
hour A B C Av Av-bl std dev %cv df [IFN]
2 16.6 17.2 17.1 16.97 10.77 0.32 1.89 2 37.62
4 43.4 43.9 43.7 43.67 37.47 0.25 0.58 2 112.13
6 3.5 4.6 4.7 4.27 -1.93 0.67 15.61 2 2.17
 10 62 61.1 61.1 61.4 55.2 0.52 0.85 2 161.63
14 76.6 77.6 77.2 77.13 70.93 0.5 0.65 2 205.54
18 70.7 69.4 70.7 70.27 64.07 0.75 1.07 2 186.37

IB
hour A B C Av Av-bl std dev %cv df [IFN]
2 5.1 5.2 5.4 5.23 -0.3 0.15 2.92 20 0.88
4 8 8.7 7.6 8.1 2.57 0.56 6.87 20 3.15
6 18.1 17.7 17.3 17.7 12.17 0.4 2.26 20 10.77
14 25 24.7 24.8 24.83 19.3 0.15 0.62 20 16.43
18 14.5 14.4 14.7 14.53 9 0.15 1.05 20 8.26

sample 12

Pr
hour A B C Av Av-bl std dev %cv df [IFN]
2 19.1 18.6 17.9 18.53 12.33 0.6 3.25 2 41.99
4 19.5 20 19.5 19.67 13.47 0.29 1.47 2 45.15
6 16.6 15.3 15.2 15.7 9.5 0.78 4.97 2 34.08
10 57.5 56.8 56.6 56.97 50.77 0.47 0.83 2 149.25
14 55.2 56 55.6 55.6 49.4 0.4 0.72 2 145.44
18 78.6 80 78.4 79 72.8 0.87 1.1 2 210.75

TC
hour A B C Av Av-bl std dev %cv df [IFN]
2 22.6 22.1 22.1 22.27 16.07 0.29 1.3 2 52.41
4 45.3 45.2 45.1 45.2 39 0.1 0.22 2 116.41
6 45.8 44.7 45 45.17 38.97 0.57 1.26 2 116.32
10 69.7 70.9 70.6 70.4 64.2 0.62 0.89 2 186.75
14 83.9 83.1 84 83.67 77.47 0.49 0.59 2 223.77
18 80.7 80.6 81 80.77 74.57 0.21 0.26 2 215.68

IB
hour A B C Av Av-bl std dev %cv df [IFN]
2 7.1 6.5 7 6.87 1.33 0.32 4.68 20 2.18
4 12.2 13.9 14.1 13.4 7.87 1.04 7.79 20 7.36
6 12.5 12.6 12.55 7.02 0.07 0.56 20 6.69
14 16.4 15.8 16.1 10.57 0.42 2.64 20 9.5
18 19.2 19.7 19.1 19.33 13.8 0.32 1.66 20 12.07

sample 7

Pr
hour A B C Av Av-bl std dev %cv df [IFN]
2 47.5 47 46.5 47 40.8 0.5 1.06 2 121.44
4 69.4 70 69.2 69.53 63.33 0.42 0.6 2 184.33
6 59.7 58.2 57.4 58.43 52.23 1.17 2 2 153.35
10 161.2 160.4 160 160.53 154.33 0.61 0.38 2 438.3
14 110.2 109.9 109.1 109.73 103.53 0.57 0.52 2 296.52
 18 101 101.6 101.7 101.43 95.23 0.38 0.37 2 273.36

TC
hour A B C Av Av-bl std dev %cv df [IFN]
2 24.5 25.5 25.3 25.1 18.9 0.53 2.11 2 60.32
4 42.3 42.9 42.5 42.57 36.37 0.31 0.72 2 109.06
6 37.5 37.6 36.8 37.3 31.1 0.44 1.17 2 94.37
10 58 57.5 57.7 57.73 51.53 0.25 0.44 2 151.39
14 66.6 66.8 66.6 66.67 60.47 0.12 0.17 2 176.33
18 59.4 57.9 58.2 58.5 52.3 0.79 1.36 2 153.53

IB
hour A B C Av Av-bl std dev %cv df [IFN]
2 25.7 27.7 27.1 26.83 21.3 1.03 3.82 20 18.02
4 11.8 11.4 11.6 11.6 6.07 0.2 1.72 20 5.93
6 10 10.1 9.9 10 4.47 0.1 1 20 4.66
14 24.5 23.7 23.9 24.03 18.5 0.42 1.73 20 15.8
18 14.7 14.6 15.4 14.9 9.37 0.44 2.93 20 8.55

sample 8
Pr
hour A B C Av Av-bl std dev %cv df [IFN]
2 66.3 66.1 65.5 65.97 59.77 0.42 0.63 2 174.37
4 65.1 65.3 63.1 64.5 58.3 1.22 1.89 2 170.28
6 92.3 90.8 90.1 91.07 84.87 1.12 1.23 2 244.43
10 127.2 126.6 125.1 126.3 120.1 1.08 0.86 2 342.76
14 108.7 109.8 109.7 109.4 103.2 0.61 0.56 2 295.59
18 50.4 49.5 48.7 49.53 43.33 0.85 1.72 2 128.51

TC
hour A B C Av Av-bl std dev %cv df [IFN]
2 28.2 27.9 27.9 28 21.8 0.17 0.62 2 68.41
4 50.2 48.8 48.1 49.03 42.83 1.07 2.18 2 127.11
6 36.4 36.6 35.8 36.27 30.07 0.42 1.15 2 91.48
10 57.4 57.6 58.6 57.87 51.67 0.64 1.11 2 151.77
14 63.3 62.7 64.2 63.4 57.2 0.75 1.19 2 167.21
18 56.3 55.2 54.9 55.47 49.27 0.74 1.33 2 145.07

IB
hour A B C Av Av-bl std dev %cv df [IFN]
2 23.8 27 26.4 25.73 20.2 1.7 6.61 20 17.15
4 11.7 12.9 12.3 6.77 0.85 6.9 20 6.49
6 11.6 11.5 11.55 6.02 0.07 0.61 20 5.89
14 18.1 18.4 18.25 12.72 0.21 1.16 20 11.21
18 13 12.8 13.1 12.97 7.43 0.15 1.18 20 7.02

sample 15
Pr
hour A B C Av Av-bl std dev %cv df [IFN]
2 49.5 49 48.6 49.03 42.83 0.45 0.92 2 127.11
4 76.9 76.7 76.4 76.67 70.47 0.25 0.33 2 204.24
6 66.2 65.7 64.4 65.43 59.23 0.93 1.42 2 172.88
10 89.2 88.9 89.8 89.3 83.1 0.46 0.51 2 239.5
14 103.7 103.7 102.4 103.27 97.07 0.75 0.73 2 278.48
18 45.2 45.4 45.1 45.23 39.03 0.15 0.34 2 116.51

TC
hour A B C Av Av-bl std dev %cv df [IFN]
2 29.9 30.3 29.8 30 23.8 0.26 0.88 2 73.99
4 53.2 54 52.9 53.37 47.17 0.57 1.07 2 139.21
6 46.3 46.8 47.1 46.73 40.53 0.4 0.86 2 120.69
10 78.4 78.1 78.6 78.37 72.17 0.25 0.32 2 208.98
14 70.5 70.8 70.8 70.7 64.5 0.17 0.24 2 187.58
18 77.3 76.2 76.1 76.53 70.33 0.67 0.87 2 203.86

IB
hour A B C Av Av-bl std dev %cv df [IFN]
2 16.8 16 17.1 16.63 11.1 0.57 3.42 20 9.93
4 11.1 10.6 11 10.9 5.37 0.26 2.43 20 5.38
6 13.9 13.3 13.1 13.43 7.9 0.42 3.1 20 7.39
14 23.8 23.9 24.5 24.07 18.53 0.38 1.57 20 15.82
18 13.8 13.8 13.7 13.77 8.23 0.06 0.42 20 7.65

sample 16

Pr
hour A B C Av Av-bl std dev %cv df [IFN]
2 49.9 49.5 47.9 49.1 42.9 1.06 2.16 2 127.3
4 66.9 65.8 64.8 65.83 59.63 1.05 1.6 2 174
6 64.5 63.3 62.2 63.33 57.13 1.15 1.82 2 167.02
10 94.3 94.8 94.3 94.47 88.27 0.29 0.31 2 253.92
14 80 79.7 79.2 79.63 73.43 0.4 0.51 2 212.52
18 49.4 49 48.6 49 42.8 0.4 0.82 2 127.02

TC
hour A B C Av Av-bl std dev %cv df [IFN]
2 45.1 44.4 44.3 44.6 38.4 0.44 0.98 2 114.74
4 56.9 56.5 56.9 56.77 50.57 0.23 0.41 2 148.7
6 53 52.4 52.7 52.7 46.5 0.3 0.57 2 137.35
10 89.4 89.1 90.3 89.6 83.4 0.62 0.7 2 240.33
14 127.6 122.6 124.5 124.9 118.7 2.52 2.02 2 338.85
18 88.5 87.7 87.9 88.03 81.83 0.42 0.47 2 235.96

IB
hour A B C Av Av-bl std dev %cv df [IFN]
 2 12.9 12.6 12.3 12.6 7.07 0.3 2.38 20 6.72
4 11.9 12.1 12 12 6.47 0.1 0.83 20 6.25
6 14.2 13.6 13.9 8.37 0.42 3.05 20 7.76
14 27.6 27.7 27.65 22.12 0.07 0.26 20 18.67
18 16.5 16.1 16.2 16.27 10.73 0.21 1.28 20 9.63
 std curve (lower)
140
f(x) = 1.26x - 1.41
120 R² = 1
100
80
60
40
20
0
0 50 100 150 200 250

std curve (higher)

800
f(x) = 0.36x - 2.71
700 R² = 1
600
500
400
300
200
100
0
0 500 1000 1500 2000 2500

/10
[IFN]*df /2 repeat actual
46.21 23.1 2.31
13.64 6.82 below limit 20.86 0.68
107.42 53.71 5.37
254.6 127.3 12.73
790.83 395.42 39.54
276.92 138.46 13.85

[IFN]*df /2 actual
72.63 36.31 3.63
110.58 55.29 5.53
151.7 75.85 7.59
197.66 98.83 9.88
244.36 122.18 12.22
223.15 111.58 11.16

[IFN]*df /2 actual
28.7 14.35 1.44
9.13 4.56 0.46
91.12 45.56 4.56
142.96 71.48 7.15
120.21 60.11 6.01
 0

[IFN]*df /2 actual
58.67 29.34 2.93
44.16 22.08 2.21
105.19 52.59 5.26
444.2 222.1 22.21
1212.83 606.41 60.64
570.53 285.27 28.53

[IFN]*df /2 actual
64.25 32.13 3.21
103.14 51.57 5.16
120.63 60.32 6.03
214.22 107.11 10.71
377.03 188.51 18.85
297.58 148.79 14.88

[IFN]*df /2 actual
26.59 13.29 1.33
25.53 12.76 1.28
82.13 41.06 4.11
254.31 127.15 12.72
177.34 88.67 8.87
0

[IFN]*df /2 actual
56.63 28.31 2.83
51.23 25.61 2.56
29.65 14.82 below limit 26.75 2.68
236.36 118.18 11.82
273.39 136.69 13.67
248.64 124.32 12.43

[IFN]*df /2 actual
75.23 37.62 3.76
224.27 112.13 11.21
4.34 2.17 below limit 0.22
 323.25 161.63 16.16
411.08 205.54 20.55
372.75 186.37 18.64

[IFN]*df /2 actual
17.59 8.8 0.88
63.08 31.54 3.15
215.43 107.71 10.77
328.63 164.31 16.43
165.18 82.59 8.26
0

[IFN]*df /2 actual
83.98 41.99 4.2
90.3 45.15 4.52
68.16 34.08 3.41
298.51 149.25 14.93
290.88 145.44 14.54
421.5 210.75 21.07

[IFN]*df /2 actual
104.82 52.41 5.24
232.83 116.41 11.64
232.64 116.32 11.63
373.49 186.75 18.67
447.55 223.77 22.38
431.36 215.68 21.57

[IFN]*df /2 actual
43.51 21.76 2.18
147.19 73.6 7.36
133.7 66.85 6.69
190.04 95.02 9.5
241.35 120.67 12.07
0

[IFN]*df /2 actual
242.88 121.44 12.14 12.14 24.29
368.65 184.33 18.43
306.7 153.35 15.33
876.61 438.3 43.83
593.05 296.52 29.65
 546.72 273.36 27.34

[IFN]*df /2 actual
120.63 60.32 6.03
218.13 109.06 10.91
188.73 94.37 9.44
302.79 151.39 15.14
352.65 176.33 17.63
307.07 153.53 15.35

[IFN]*df /2 actual
360.37 180.18 18.02
118.63 59.31 5.93
93.24 46.62 4.66
315.93 157.97 15.8
171 85.5 8.55

[IFN]*df /2 actual
348.75 174.37 17.44
340.56 170.28 17.03
488.85 244.43 24.44
685.52 342.76 34.28
591.19 295.59 29.56
257.02 128.51 12.85

[IFN]*df /2 actual
136.82 68.41 6.84
254.23 127.11 12.71
182.96 91.48 9.15
303.53 151.77 15.18
334.42 167.21 16.72
290.14 145.07 14.51

[IFN]*df /2 actual
342.91 171.46 17.15
129.74 64.87 6.49
117.83 58.92 5.89
224.16 112.08 11.21
140.31 70.16 7.02
[IFN]*df /2 actual
254.23 127.11 12.71
408.47 204.24 20.42
345.77 172.88 17.29
478.99 239.5 23.95
556.95 278.48 27.85
233.01 116.51 11.65

[IFN]*df /2 actual
147.98 73.99 7.4
278.41 139.21 13.92
241.39 120.69 12.07
417.96 208.98 20.9
375.17 187.58 18.76
407.73 203.86 20.39

[IFN]*df /2 actual
198.5 99.25 9.93
107.52 53.76 5.38
147.72 73.86 7.39
316.46 158.23 15.82
153.01 76.51 7.65

/10
[IFN]*df /2 actual
254.6 127.3 12.73
348 174 17.4
334.05 167.02 16.7
507.83 253.92 25.39
425.03 212.52 21.25
254.04 127.02 12.7

[IFN]*df /2 actual
229.48 114.74 11.47
297.39 148.7 14.87
274.69 137.35 13.73
480.66 240.33 24.03
677.71 338.85 33.89
471.92 235.96 23.6

[IFN]*df /2 actual
 134.5 67.25 6.72
124.97 62.49 6.25
155.13 77.56 7.76
373.33 186.66 18.67
192.68 96.34 9.63
std curve 140
f(x) = 1.26x - 1.4
120 R² = 1
IFN A B C Av Av-bl
100
0 5.3 5.7 5.6 5.53 0 Y=1.2603X - 1.4086
80
6.25 13.6 13.3 13.8 13.57 8.03
12.5 20.1 19.6 20.2 19.97 14.43 60
25 33.9 33.7 32.9 33.5 27.97 40
50 64.6 64.4 64.3 64.43 58.9 20
100 132.5 132.6 130.7 131.93 126.4
0
200 359 357.8 349.4 0

800
0 5.7 6.7 6.2 0 Y= 0.3583x - 2.7111 f(x) = 0.36x - 2.7
15.6 10 9 9.5 3.3 700 R² = 1
31.25 14.8 16.8 15.8 9.6 600
62.5 27.5 27.5 21.3
500
125 48.4 48.6 48.5 42.3
250 91.7 91.7 91.7 85.5 400
500 179.1 178.5 178.8 172.6 300
1000 357.8 358.2 358 351.8 200
2000 718.5 727.7 723.1 716.9
100

0
0

pr av av-blank std dev %cv [IFN] df

4h pr 23' 901.5 900.4 903.2 901.70 895.50 1.41 0.16 2506.87 2
4h pr 24' 805.8 806.1 817.2 809.70 803.50 6.50 0.80 2250.10 2
6h Pr 23' 1252.9 1184.2 1167.5 1175.85 1169.65 11.81 1.00 3272.01 2
6h Pr 24' 1027 1038.7 1036.5 1034.07 1027.87 6.22 0.60 2876.30 2
8h pr 23' 868.5 867.8 854.8 863.70 857.50 7.72 0.89 2400.81 2
8h pr 24' 786.9 786.9 780.5 784.77 778.57 3.70 0.47 2180.51 2
10h pr 23' 299.2 306.2 301.7 302.37 296.17 3.55 1.17 834.16 2
10h pr 24' 249.1 250.6 242.8 247.50 241.30 4.14 1.67 681.02 2
14h pr 23' 1404.6 1253 1235.5 1297.70 1291.50 92.99 7.17 3612.09 2
14h pr 24' 1230.6 1225.2 1219.4 1225.07 1218.87 5.60 0.46 3409.37 2
18h pr 23' 1238.6 1230.5 1228.5 1232.53 1226.33 5.35 0.43 3430.21 2
18h pr 24' 1224.3 1224.3 1223.2 1223.93 1217.73 0.64 0.05 3406.21 2

tc av av-blank std dev %cv [IFN] df

4h tc 23' 484.4 688.8 786.1 653.10 646.90 153.99 23.58 1813.04 2
4h tc 24' 849.1 803.3 849.7 834.03 827.83 26.62 3.19 2318.01 2
6h tc 23' 897.3 910.1 894.8 900.73 894.53 8.21 0.91 2504.17 2
6h tc 24' 852.6 866.9 859.2 859.57 853.37 7.16 0.83 2389.28 2
8h tc 23' 851.3 850.7 842.4 848.13 841.93 4.97 0.59 2357.37 2
8h tc 24' 1058.9 961.7 928.3 982.97 976.77 67.85 6.90 2733.68 2
10h tc 23' 347.4 359.5 354.2 353.70 347.50 6.07 1.71 977.42 2
10h tc 24' 329.9 329 333.1 330.67 324.47 2.15 0.65 913.14 2
14h tc 23' 1377.8 1233.2 1202 1271.00 1264.80 93.80 7.38 3537.57 2
14h tc 24' 1067.2 1087.1 1073.6 1075.97 1069.77 10.16 0.94 2993.24 2
18h tc 23' 1201.8 1190.7 1182.1 1191.53 1185.33 9.88 0.83 3315.78 2
18h tc 24' 1154.4 1166 1158.2 1159.53 1153.33 5.91 0.51 3226.47 2

ib Y=1.2603X - 1.4086

Sample av av-blank std dev %cv [IFN] df

8h ib 23' 234.2 254.5 244.35 238.82 14.35 5.87 190.61 20
8h ib 24' 214 209.4 211.70 206.17 3.25 1.54 164.7 20
10h ib 23' 69.6 73.7 71.65 66.12 2.90 4.05 53.58 20
10h ib 24' 64.3 70.6 67.45 61.92 4.45 6.60 50.25 20
14h ib 23' 481 464.4 472.70 467.17 11.74 2.48 371.8 20
14h ib 24 447.4 442.7 445.05 439.52 3.32 0.75 349.86 20
18h ib 23' 472.3 471.3 471.80 466.27 0.71 0.15 371.08 20
18h ib 24' 623.5 619.6 621.55 616.02 2.76 0.44 489.9 20

sample pr tc ib temp OD iptg

23 8 240.08 235.74 190.61 25 4 1.03
10 83.42 97.74 53.58
14 361.21 353.76 371.8
18 343.02 331.58 371.08

24 8 218.05 273.37 164.7 25 4 1.03

10 68.1 91.31 50.25
14 340.94 299.32 349.86
18 340.62 322.65 389.9

sample OD 600 DCW temp OD iptg

20 13.142 7.695 25 8 1.03
11.798 6.874

21 13.840 8.122 25 4 0
11.200 6.509

22 14.610 8.592 25 4 2.66

11.822 6.889

rpt (200209) av av-blank std dev %cv [IFN] df [ifn]*df

26 14h pr 625 620.1 622.55 616.35 3.46 0.56 1727.77 2 3455.55
25 14h pr 652.8 646.9 649.85 643.65 4.17 0.64 1803.97 2 3607.93
22 14h pr 687.9 690.4 689.15 682.95 1.77 0.26 1913.65 2 3827.30
26 18h pr 766.5 689.6 728.05 721.85 54.38 7.47 2022.22 2 4044.44
25 18h pr 705.6 687.4 696.50 690.30 12.87 1.85 1934.16 2 3868.33
22 18h pr 693.9 687.6 690.75 684.55 4.45 0.64 1918.12 2 3836.23
26 14h tc 424.2 447.6 435.90 429.70 16.55 3.80 1206.84 2 2413.68
25 14h tc 450.8 481.1 465.95 459.75 21.43 4.60 1290.71 2 2581.42
22 14h tc 600 548.9 574.45 568.25 36.13 6.29 1593.53 2 3187.06
26 18h tc 638.6 629.1 633.85 627.65 6.72 1.06 1759.31 2 3518.62
25 18h tc 477.1 471.9 474.50 468.30 3.68 0.77 1314.57 2 2629.14
22 18h tc 498.4 447.2 472.80 466.60 36.20 7.66 1309.83 2 2619.65

av av-blank std dev %cv [IFN] df [ifn]*df

22 14h ib 3.4 3.4 3.4 -2.13 0.00 0.00 -0.58 20 -11.50
25 14h ib 2.6 7.2 4.9 -0.63 3.25 66.38 0.62 20 12.30
26 14h ib 9.2 9.7 9.45 3.92 0.35 3.74 4.23 20 84.51
22 18h ib 10.3 12.1 11.2 5.67 1.27 11.36 5.61 20 112.28
25 18h ib 11.7 12.1 11.9 6.37 0.28 2.38 6.17 20 123.39
26 18h ib 10.5 10.3 10.4 4.87 0.14 1.36 4.98 20 99.58
140
f(x) = 1.26x - 1.41
120 R² = 1
100
80
60
40
20
0
0 50 100 150 200 250

800
f(x) = 0.36x - 2.71
700 R² = 1

600

500

400

300

200

100

0
0 500 1000 1500 2000 2500
/2 /10
[ifn]*df conc sample
actual [ifn]
5013.74 2506.87 250.69
4500.20 2250.10 225.01
6544.02 3272.01 327.20
5752.60 2876.30 287.63
4801.62 2400.81 240.08
4361.03 2180.51 218.05
1668.31 834.16 83.42
1362.05 681.02 68.10
7224.18 3612.09 361.21
6818.74 3409.37 340.94
6860.42 3430.21 343.02
6812.42 3406.21 340.62

/2 /10
[ifn]*df conc sample
actual [ifn]
3626.07 1813.04 181.30
4636.03 2318.01 231.80
5008.34 2504.17 250.42
4778.55 2389.28 238.93
4714.73 2357.37 235.74
5467.36 2733.68 273.37
1954.85 977.42 97.74
1826.28 913.14 91.31
7075.14 3537.57 353.76
5986.48 2993.24 299.32
6631.56 3315.78 331.58
 6452.94 3226.47 322.65

/2 /10
[ifn]*df conc sample
actual [ifn]
3812.19 1906.10 190.61
3294.06 1647.03 164.70
1071.57 535.79 53.58
1004.92 502.46 50.25
7435.93 3717.97 371.80
6997.15 3498.57 349.86
7421.65 3710.82 371.08
9798.07 4899.03 489.90

/2 /10
conc sampleactual [ifn] rpt (200209)actual [ifn]
1727.77 172.78 26 14h pr 172.78
1803.97 180.40 25 14h pr 180.4
1913.65 191.37 22 14h pr 191.37
2022.22 202.22 26 18h pr 202.22
1934.16 193.42 25 18h pr 193.42
1918.12 191.81 22 18h pr 191.81
1206.84 120.68 26 14h tc 120.68
1290.71 129.07 25 14h tc 129.07
1593.53 159.35 22 14h tc 159.35
1759.31 175.93 26 18h tc 175.93
 1314.57 131.46 25 18h tc 131.46
1309.83 130.98 22 18h tc 130.98

/2 /10 /10
conc sampleactual [ifn] actual [ifn]
-5.75 -0.58 22 14h ib -0.58
6.15 0.62 25 14h ib 0.62
42.25 4.23 26 14h ib 4.23
56.14 5.61 22 18h ib 5.61
61.69 6.17 25 18h ib 6.17
49.79 4.98 26 18h ib 4.98
sample hour pr tc temp OD iptg
1 8 8.93 2.31 0 20 1 0.05
10 17.7 9.13 3.59
14 9.34 3.49 4.82
18 7.99 5.43 0

3 2 2.31 3.63 1.44 30 7 0.05

4 0.68 5.53 0.46
6 5.37 7.59 4.56
10 12.73 9.88
14 39.54 12.22 7.15
18 13.85 11.16 6.01

4 2 2.93 3.21 1.33 30 7 0.05

4 2.21 5.16 1.28
6 5.26 6.03 4.11
10 22.21 10.71
14 60.64 18.85 12.72
18 28.53 14.88 8.87

5 8 13.33 36.36 11.84 20 1 0.05

10 20.07 15.43 4.82
14 59.15 18.45 3.83
18 148.29 55.81 17

6 8 40.43 25.86 14.62 20 1 0.05

10 31.88 8.08 6.61
14 74.05 21.94 14.66
18 119.12 44.18 11.64

7 2 12.14 6.03 18.02 30 7 0.05

4 18.43 10.91 5.93
6 15.33 9.44 4.66
10 43.83 15.14
14 29.65 17.63 15.8
18 27.34 15.35 8.55

8 2 17.44 6.84 17.15 30 7 0.05

4 17.03 12.71 6.49
6 24.44 9.15 5.89
10 34.28 15.18
14 29.56 16.72 11.21
18 12.85 14.51 7.02

9 8 7.59 6.6 5.73 20 1 2

10 30.56 15.48 0.46
14 9.5 21.81 2.52
18 9.6 28.02 2.32
 10 10 27.34 11.77 4.3 20 1 2
14 12.68 34.42 5.85
18 16.64 32.05 0.65

11 2 2.83 3.76 0.88 30 7 2

4 2.56 11.21 3.15
6 2.68 0.22 10.77
10 11.82 16.16
14 13.67 20.55 16.43
18 12.43 18.64 8.26

12 2 4.2 5.24 2.18 30 7 2

4 4.52 11.64 7.36
6 3.41 11.63 6.69
10 14.93 18.67
14 14.54 22.38 9.5
18 21.07 21.57 12.07

13 8 18.66 19.82 20 1 2
10 33.36 23.7 6.53
14 56.67 32.13 11.09
18 194.04 81.42 21.68

14 8 31.31 26.52 20 1 2
10 108.6 19.22 6.45
14 97.02 36.06 6.96
18 244.89 58.08 16.68

15 2 12.71 7.4 9.93 30 7 2

4 20.42 13.92 5.38
6 17.29 12.07 7.39
10 23.95 20.9
14 27.85 18.76 15.82
18 11.65 20.39 7.65

16 2 12.73 11.47 6.72 30 7 2

4 17.4 14.87 6.25
6 16.7 13.73 7.76
10 25.39 24.03
14 21.25 33.89 18.67
18 12.7 23.6 9.63

sample pr tc ib temp OD iptg

23 8 240.08 235.74 190.61 25 4 1.03
10 83.42 97.74 53.58
14 361.21 353.76 371.8
18 343.02 331.58 371.08
24 8 218.05 273.37 164.7 25 4 1.03
10 68.1 91.31 50.25
14 340.94 299.32 349.86
18 340.62 322.65 389.9
IFN A B C Av Av-bl
0 5.3 5.7 5.6 5.533 0 Y=1.2603X - 1.4086
6.25 13.6 13.3 13.8 13.567 8.033
12.5 20.1 19.6 20.2 19.967 14.433
25 33.9 33.7 32.9 33.500 27.967
50 64.6 64.4 64.3 64.433 58.900
100 132.5 132.6 130.7 131.933 126.400
200 359 357.8 349.4

0 5.7 6.7 6.2 0 Y= 0.3583x - 2.7111

15.6 10 9 9.5 3.3
31.25 14.8 16.8 15.8 9.6
62.5 27.5 27.5 21.3
125 48.4 48.6 48.5 42.3
250 91.7 91.7 91.7 85.5
500 179.1 178.5 178.8 172.6
1000 357.8 358.2 358 351.8
2000 718.5 727.7 723.1 716.9

pr Av av-bl std dev %cv df [ifn]

8h pr 1 103.8 119.1 129 117.300 111.100 12.696 10.824 2 89.27
8h pr 9 89.5 103.4 108.4 100.433 94.233 9.793 9.751 2 75.89
'8h pr 5' 47.3 52.6 53.9 51.267 45.067 3.496 6.820 2 133.346
'8h pr 6' 151.5 146.8 146.8 148.367 142.167 2.714 1.829 2 404.348
'8h pr 13' 69.9 71 70.1 70.333 64.133 0.586 0.833 2 186.560
'8h pr 14' 114.7 117.2 115.1 115.667 109.467 1.343 1.161 2 313.083

Av av-bl std dev %cv df [ifn]

10h pr 1 208.4 230.2 245 227.867 221.667 18.411 8.080 2 177
10h pr 9 378.2 383.9 407.7 389.933 383.733 15.648 4.013 2 305.6
10h pr 10 334 348.7 365.5 349.400 343.200 15.762 4.511 2 273.43
'10h pr 5' 75.6 75.8 74.8 75.4 69.2 0.53 0.7 2 200.701
'10h pr 6' 117.4 117.1 118.6 117.7 111.5 0.79 0.67 2 318.758
'10h pr 13' 123.8 122.5 122.7 123 116.8 0.7 0.57 2 333.550
'10h pr 14' 395.7 392.3 389.8 392.6 386.4 2.96 0.75 2 1085.992

Av av-bl std dev %cv df [ifn]

'14h pr 1' 125.7 118 121.85 116.32 5.44 4.47 2 93.41
'14h pr 9' 37.1 37.9 37.6 37.533 31.333 0.404 1.077 2 95.017
'14h pr 10' 50 48.9 47.9 48.933 42.733 1.050 2.147 2 126.833
'14h pr 5' 216.4 215.7 214.2 215.433 209.233 1.124 0.522 2 591.528
'14h pr 6' 268.1 269.1 269.2 268.800 262.600 0.608 0.226 2 740.472
'14h pr 13' 209.2 203.9 206.550 200.350 3.748 1.814 2 566.735
'14h pr 14' 357.8 344.4 351.100 344.900 9.475 2.699 2 970.168

Av av-bl std dev %cv df [ifn]

'18h pr 1' 33.9 30.3 32.1 25.900 2.546 7.930 2 79.852
'18h pr 9' 38.5 37.3 37.9 31.700 0.849 2.239 2 96.040
'18h pr 10' 64.3 61.9 63.1 56.900 1.697 2.689 2 166.372
'18h pr 5' 549.8 519.8 534.8 528.600 21.213 3.967 2 1482.867
'18h pr 6' 435.4 425.2 430.3 424.100 7.212 1.676 2 1191.212
'18h pr 13' 724.1 673.4 698.75 692.550 35.850 5.131 2 1940.444
18h pr 14 (10x)' ib
627.6
template 618.8 617.8 621.4 615.87 5.393 0.868 10 489.784

tc
Sample RelResp av av-bl std dev %cv df [ifn]
8h1TC 11.4 12.6 11.3 11.767 5.567 0.723 6.148 2 23.103
8h9TC 27.9 27.6 25.9 27.133 20.933 1.079 3.975 2 65.991
8h5TC 130.5 147.7 123.1 133.767 127.567 12.621 9.435 2 363.600
8h6TC 100.2 96 92.2 96.133 89.933 4.002 4.163 2 258.567

10h1TC 37.5 36 35.1 36.200 30.000 1.212 3.349 2 91.295

10h9TC 58.5 59.5 58.9 58.967 52.767 0.503 0.854 2 154.836
10h10TC 47.7 45.4 43.9 45.667 39.467 1.914 4.191 2 117.716
10h5TC 57.6 58.7 60 58.767 52.567 1.201 2.044 2 154.278
10h6TC 29.8 33.1 34.4 32.433 26.233 2.371 7.311 2 80.783
10h13TC 86.7 89.1 89.4 88.400 82.200 1.480 1.674 2 236.983
14h10TC1 70.2 73.6 73.3 72.367 66.167 1.882 2.601 2 192.235

14h1TC 17.1 15.4 15.5 16.000 9.800 0.954 5.962 2 34.918

14h9TC 80.2 82.9 81.8 81.633 75.433 1.358 1.663 2 218.098
14h10TC2 116.4 133.4 130.7 126.833 120.633 9.136 7.203 2 344.249
14h5TC 67.4 70.9 70.5 69.600 63.400 1.916 2.752 2 184.513
14h6TC 74 86.5 85.8 82.100 75.900 7.024 8.555 2 219.400
14h13TC 119.1 116.1 120.6 118.600 112.400 2.291 1.932 2 321.270
14h14TC 131 133.8 133.3 132.700 126.500 1.493 1.125 2 360.623

18h1TC 22.3 23.1 23.4 22.933 16.733 0.569 2.479 2 54.269

18h9TC 104.9 105.2 101.6 103.900 97.700 1.997 1.923 2 280.243
18h10TC 113 122.2 119.8 118.333 112.133 4.772 4.033 2 320.526
18h5TC 197.6 206.1 206.7 203.467 197.267 5.090 2.501 2 558.129
18h6TC 160.3 162.4 162.7 161.800 155.600 1.308 0.808 2 441.840
18h13TC 296.8 297.1 291.7 295.200 289.000 3.035 1.028 2 814.153
18h14TC 219.9 210 204.9 211.600 205.400 7.627 3.604 2 580.829

Y=1.2603X - 1.4086
ib

Sample RelResp av av-bl std dev %cv df [ifn]

'8h ib 1' 1.3 6.7 4.000 -1.533 3.818 95.459 20 -0.1
'8h ib 9' 10.3 12.4 11.350 5.817 1.485 13.083 20 5.73
'8h ib 5' 23.7 14.4 19.050 13.517 6.576 34.520 20 11.84
'8h ib 6' 25 20.1 22.550 17.017 3.465 15.365 20 14.62
'8h ib 13' 31.8 26.4 29.100 23.567 3.818 13.122 20 19.82
'8h ib 14' 38.4 36.7 37.550 32.017 1.202 3.201 20 26.52

'10h ih 1' 8.6 8.7 8.650 3.117 0.071 0.817 20 3.59

'10h ib 9' 6.7 2.7 4.700 -0.833 2.828 60.179 20 0.46
'10h ib 10' 11.1 8 9.550 4.017 2.192 22.953 20 4.3
'10h ib 5' 7.4 13 10.200 4.667 3.960 38.822 20 4.82
'10h ib 6' 13.3 11.6 12.450 6.917 1.202 9.655 20 6.61
'10h ib 13' 13.1 11.6 12.350 6.817 1.061 8.588 20 6.53
'10h ib 14' 13.2 11.3 12.250 6.717 1.344 10.967 20 6.45

'14h ib 1' 11.9 8.5 10.200 4.667 2.404 23.570 20 4.82

'14h ib 9' 7.1 7.5 7.3 7.300 1.767 0.200 2.740 20 2.52
'*14h ib 10' 14 9 11.500 5.967 3.536 30.744 20 5.85
'*14h ib 5' 8.5 9.4 8.950 3.417 0.636 7.111 20 3.83
'*14h ib 6' 22.3 22.9 22.600 17.067 0.424 1.877 20 14.66
'*14h ib 13' 19 17.2 18.100 12.567 1.273 7.032 20 11.09
'*14h ib 14' 13.5 12.3 12.900 7.367 0.849 6.578 20 6.96

'*18h ib 1' 4.8 2.8 3.80 -1.733 1.41 37.22 20 -0.26

'*18h ib 9' 7.2 6.9 7.05 1.517 0.21 3.01 20 2.32
'*18h ib 10' 3.8 6.1 6 4.95 -0.583 1.30 26.26 20 0.65
'*18h ib 5' 27 24.1 25.55 20.017 2.05 8.03 20 17
'*18h ib 6' 19.6 18 18.80 13.267 1.13 6.02 20 11.64
'*18h ib 13' 31.8 31.1 31.45 25.917 0.49 1.57 20 21.68
'*18h ib 14' 24.1 26.2 25.15 19.617 1.48 5.90 20 16.68

sample pr tc ib
1 8 8.93 2.31 0
10 17.7 9.13 3.59
14 9.34 3.49 4.82
18 7.99 5.43 0

9 8 7.59 6.6 5.73

10 30.56 15.48 0.46
14 9.5 21.81 2.52
18 9.6 28.02 2.32

10 8
10 27.34 11.77 4.3
14 12.68 34.42 5.85
18 16.64 32.05 0.65

5 8 13.33 36.36 11.84

10 20.07 15.43 4.82
14 59.15 18.45 3.83
18 148.29 55.81 17

6 8 40.43 25.86 14.62

10 31.88 8.08 6.61
14 74.05 21.94 14.66
 18 119.12 44.18 11.64

13 8 18.66 19.82
10 33.36 23.7 6.53
14 56.67 32.13 11.09
18 194.04 81.42 21.68

14 8 31.31 26.52
10 108.6 19.22 6.45
14 97.02 36.06 6.96
18 244.89 58.08 16.68
[ifn]*df /2 /10
178.543 89.271 8.927
151.776 75.888 7.589
266.691 133.346 13.335
808.695 404.348 40.435
373.120 186.560 18.656
626.167 313.083 31.308

[ifn]*df /2 /10
354.003 177.002 17.700
611.191 305.595 30.560
546.868 273.434 27.343
401.402 200.701 20.070
637.517 318.758 31.876
667.101 333.550 33.355
2171.985 1085.992 108.599

[ifn]*df /2 /10
186.821 93.41 9.34
190.033 95.017 9.502
253.667 126.833 12.683
1183.056 591.528 59.153
1480.944 740.472 74.047
1133.470 566.735 56.673
1940.335 970.168 97.017

[ifn]*df /2 /10
159.705 79.852 7.985
192.080 96.040 9.604
 332.744 166.372 16.637
2965.733 1482.867 148.287
2382.423 1191.212 119.121
3880.888 1940.444 194.044
4897.844 2448.92 244.89

[ifn]*df /2 /10
46.206 23.103 2.310
131.981 65.991 6.599
727.199 363.600 36.360
517.133 258.567 25.857

182.591 91.295 9.130

309.672 154.836 15.484
235.433 117.716 11.772
308.556 154.278 15.428
161.565 80.783 8.078
473.967 236.983 23.698
384.470 192.235 19.223

69.836 34.918 3.492

436.196 218.098 21.810
688.498 344.249 34.425
369.027 184.513 18.451
438.800 219.400 21.940
642.540 321.270 32.127
721.245 360.623 36.062

108.537 54.269 5.427

560.486 280.243 28.024
641.052 320.526 32.053
1116.259 558.129 55.813
883.679 441.840 44.184
1628.306 814.153 81.415
1161.658 580.829 58.083

[ifn]*df /2 /10
-1.979 -0.990 0.000
114.659 57.330 5.733
236.853 118.426 11.843
292.395 146.197 14.620
396.338 198.169 19.817
530.433 265.217 26.522

71.813 35.906 3.591

9.129 4.565 0.456
86.095 43.047 4.305
96.410 48.205 4.820
132.116 66.058 6.606
130.529 65.264 6.526
128.942 64.471 6.447

96.410 48.205 4.820

50.389 25.195 2.519
117.040 58.520 5.852
76.573 38.287 3.829
293.188 146.594 14.659
221.777 110.888 11.089
139.257 69.628 6.963

-5.153 -2.577 -0.258

46.422 23.211 2.321
13.096 6.548 0.655
340.003 170.001 17.000
232.885 116.443 11.644
433.631 216.816 21.682
333.655 166.827 16.683
Protein

Pr TC Ibs
sample Ind hour [protein] [protein] [protein] OD 600
3 2 0.018 0.034 0.000 3.64
4 0.004 0.051 0.000 3.66
6 0.030 0.065 0.000 7.23
10 0.137 0.214 0.095 11.72
14 0.224 0.289 0.000 13.48
18 0.111 0.324 0.047 11.84

4 2 0.018 0.062 0.000 3.58

4 0.011 0.082 0.000 5.61
6 0.032 0.117 0.000 7.62
10 0.179 0.106 0.107 11.92
14 0.263 0.279 0.052 14.01
18 0.162 0.304 0.000 12.8

11 2 0.014 0.067 0.000 3.34

4 0.013 0.085 0.000 3.05
6 0.013 0.000 0.000 5.4
10 0.066 0.219 0.000 8.04
14 0.119 0.228 0.000 9.31
18 0.153 0.211 0.000 9.79

12 2 0.016 0.068 0.000 3.64

4 0.006 0.087 0.000 2.89
6 0.018 0.072 0.000 5.95
10 0.069 0.226 0.000 7.88
14 0.079 0.220 0.000 8.43
18 0.231 0.204 0.000 10.44

7 2 0.147 0.149 0.000 12.64

4 0.087 0.147 0.000 13.75
6 0.081 0.162 0.000 12.15
10 0.257 0.371 0.000 13.96
14 0.225 0.316 0.049 16.05
18 0.259 0.200 0.000 12.66

8 2 0.119 0.182 0.000 12.8

4 0.084 0.135 0.000 12.51
6 0.094 0.177 0.000 14.67
10 0.265 0.317 0.050 13.98
14 0.267 0.328 0.049 15.47
18 0.150 0.162 0.000 13.25

15 2 0.103 0.190 0.000 11.91

4 0.060 0.107 0.000 13.01
6 0.100 0.111 0.000 13.53
10 0.202 0.350 0.000 13.22
 14 0.155 0.292 0.000 13.04
18 0.145 0.194 0.000 13.13

16 2 0.140 0.157 0.000 12.52

4 0.078 0.148 0.000 11.29
6 0.096 0.142 0.000 13.64
10 0.201 0.355 0.047 12.49
14 0.209 0.315 0.000 13.09
18 0.121 0.199 0.000 12.8
 av av-bl
blank 0.37 0.37 0.36 0.36 0
0.1 0.49 0.48 0.49 0.48 0.12
0.2 0.59 0.64 0.55 0.59 0.23
0.3 0.67 0.85 0.75 0.76 0.39
0.4 0.86 0.81 0.85 0.84 0.47
0.5 0.95 1.01 0.95 0.97 0.6

bradford
run 200209
pr df av av-bl std dev %cv protein conc[prot]*df
17 14h 0.54 0.54 2 0.54 0.17 0.000 0.000 0.143 0.286
18 14h 0.55 0.55 2 0.55 0.19 0.000 0.000 0.157 0.314
17 18h 0.63 0.63 2 0.63 0.27 0.000 0.000 0.222 0.444
18 18h 0.66 0.66 2 0.66 0.29 0.000 0.000 0.243 0.485
tc
17 14h 0.84 0.84 2 0.84 0.47 0.000 0.000 0.390 0.780
18 14h 0.9 0.9 2 0.9 0.53 0.000 0.000 0.439 0.877
17 18h 0.76 0.76 2 0.76 0.4 0.000 0.000 0.326 0.652
18 18h 0.87 0.87 2 0.87 0.51 0.000 0.000 0.416 0.833
ib
17 14h 0.86 0.82 1 0.84 0.48 0.029 3.449 0.393 0.393
18 14h 0.74 0.72 1 0.73 0.36 0.018 2.427 0.301 0.301
17 18h 0.67 0.65 1 0.66 0.3 0.013 2.025 0.247 0.247
18 18h 0.88 0.72 1 0.8 0.43 0.116 14.550 0.357 0.357
pr
22 14h 0.88 0.88 2 0.88 0.52 0.000 0.000 0.427 0.854
25 14h 0.92 0.92 2 0.92 0.56 0.000 0.000 0.460 0.920
22 18h 1.03 1.03 2 1.03 0.67 0.000 0.000 0.550 1.100
25 14h 0.9 0.9 2 0.9 0.54 0.000 0.000 0.441 0.882
tc
22 14h 0.73 0.73 2 0.73 0.36 0.000 0.000 0.299 0.597
25 14h 0.74 0.74 2 0.74 0.38 0.000 0.000 0.314 0.627
22 18h 0.76 0.76 2 0.76 0.4 0.000 0.000 0.328 0.655
25 14h 0.73 0.73 2 0.73 0.36 0.000 0.000 0.299 0.597
ib
22 14h 0.54 0.54 2 0.54 0.18 0.000 0.000 0.149 0.297
25 14h 0.6 0.6 2 0.6 0.24 0.000 0.000 0.194 0.388
22 18h 0.66 0.66 2 0.66 0.29 0.000 0.000 0.242 0.484
25 14h 0.71 0.71 2 0.71 0.35 0.000 0.000 0.286 0.573
actual [prot] pr actual [prot]
0.143 17 14h 0.14
0.157 18 14h 0.16
0.222 17 18h 0.22
0.243 18 18h 0.24
tc
0.390 17 14h 0.39
0.439 18 14h 0.44
0.326 17 18h 0.33
0.416 18 18h 0.42
ib
0.197 17 14h 0.2
0.150 18 14h 0.15
0.124 17 18h 0.12
0.179 18 18h 0.18
pr
0.427 22 14h 0.43
0.460 25 14h 0.46
0.550 22 18h 0.55
0.441 25 14h 0.44
tc
0.299 22 14h 0.3
0.314 25 14h 0.31
0.328 22 18h 0.33
0.299 25 14h 0.3
ib
0.149 22 14h 0.15
0.194 25 14h 0.19
0.242 22 18h 0.24
0.286 25 14h 0.29
bradford sample 25oC warning!! All sample name are change to 23 and 24

std
av av-bl
0.1 0.57 0.56 0.57 0.56
0.2 0.79 0.76 0.76 0.77 0.4
0.3 0.9 0.89 0.86 0.87 0.51
0.4 1.04 1.03 1.01 1.03 0.66
0.5 1.11 1.18 1.13 1.14 0.78
bl 0.37 0.36 0.37 0.37 0

pr df av av-blank std dev %cv protein conc

4h 17 0.93 0.97 2 0.95 0.584 0.031 3.275 0.348
4h 18 0.96 0.99 2 0.98 0.609 0.018 1.886 0.367
6h 17 0.96 0.95 2 0.95 0.588 0.008 0.816 0.350
6h 18 0.92 0.93 2 0.92 0.556 0.009 0.998 0.325
8h 17 0.75 0.75 2 0.75 0.383 0.003 0.378 0.190
8h 18 0.73 0.74 2 0.73 0.369 0.008 1.059 0.178
10h 17 0.55 0.59 0.61 2 0.58 0.218 0.032 5.413 0.060
10h 18 0.56 0.6 0.6 2 0.58 0.217 0.022 3.765 0.060
14h 17 0.92 0.95 0.94 2 0.93 0.569 0.012 1.290 0.336
14h 18 0.95 0.93 0.96 2 0.95 0.582 0.017 1.820 0.346
18h 17 0.94 0.95 2 0.94 0.576 0.004 0.450 0.341
18h 18 0.95 0.95 0.94 2 0.94 0.578 0.003 0.280 0.343

ib df av av-blank std dev %cv protein conc

4h 17 0.95 0.67 0.71 2 0.78 0.410 0.152 19.622 0.211
4h 18 0.7 0.72 0.73 2 0.72 0.351 0.015 2.024 0.164
6h 17 0.71 0.71 2 0.71 0.348 0.001 0.099 0.162
6h 18 0.71 0.79 0.75 2 0.75 0.382 0.040 5.285 0.189
8h 17 0.7 0.76 0.78 2 0.75 0.381 0.042 5.646 0.188
8h 18 0.76 0.78 2 0.77 0.404 0.020 2.571 0.206
10h 17 0.63 0.65 0.67 2 0.65 0.282 0.023 3.495 0.110
10h 18 0.63 0.66 2 0.65 0.280 0.016 2.520 0.108
14h 17 0.68 0.72 0.7 2 0.7 0.330 0.021 2.944 0.148
14h 18 0.73 0.71 0.79 2 0.74 0.377 0.040 5.422 0.185
18h 17 0.72 0.75 0.77 2 0.75 0.380 0.028 3.758 0.187
18h 18 0.72 0.73 0.72 2 0.72 0.357 0.010 1.365 0.169

tc df av av-blank std dev %cv protein conc

4h 17 0.75 0.78 0.79 4 0.77 0.405 0.020 2.633 0.207
4h 18 0.72 0.77 0.78 4 0.75 0.388 0.030 4.009 0.194
6h 17 0.79 0.78 0.86 4 0.81 0.445 0.043 5.283 0.238
6h 18 0.84 0.81 4 0.82 0.457 0.017 2.062 0.248
8h 17 0.99 0.99 4 0.99 0.625 0.003 0.285 0.380
8h 18 0.71 0.77 4 0.74 0.374 0.040 5.351 0.183
10h 17 0.63 0.71 0.66 4 0.67 0.301 0.039 5.827 0.126
10h 18 0.85 0.87 2 0.86 0.491 0.016 1.899 0.274
14h 17 0.75 0.79 4 0.77 0.401 0.030 3.967 0.203
14h 18 0.72 0.93 0.87 4 0.84 0.472 0.107 12.709 0.260
18h 17 0.68 0.9 0.91 4 0.83 0.467 0.131 15.682 0.255
18h 18 0.76 0.9 0.8 4 0.82 0.456 0.072 8.780 0.247
[prot]*df actual [prot]
0.695 0.348
0.734 0.367
0.701 0.350
0.650 0.325
0.379 0.190
0.357 0.178
0.121 0.060
0.119 0.060
0.671 0.336
0.692 0.346
0.683 0.341
0.686 0.343

[prot]*df actual [prot]

0.422 0.211 sample pr tc ib
0.329 0.164 17 8 0.190 0.760 0.188
0.324 0.162 10 0.060 0.251 0.110
0.378 0.189 14 0.336 0.407 0.148
0.377 0.188 18 0.341 0.511 0.187
0.412 0.206
0.221 0.110 18 8 0.178 0.365 0.206
0.217 0.108 10 0.060 0.274 0.108
0.297 0.148 14 0.346 0.519 0.185
0.370 0.185 18 0.343 0.494 0.169
0.374 0.187
0.338 0.169

[prot]*df actual [prot]

0.829 0.415
0.776 0.388
0.953 0.476
0.991 0.496
1.519 0.760
0.731 0.365
0.502 0.251
0.548 0.274
0.814 0.407
1.038 0.519
1.022 0.511
0.987 0.494
Protein 20

Pr TC Ibs Pr
sample Ind hour [protein] [protein] [protein] OD 600 Ind hour [protein]
1 8 0.029 0.060 0.043 3.835 3 2 0.018
10 0.041 0.091 0.016 2.185 4 0.004
14 0.018 0.109 0.105 4.637 6 0.030
18 0.045 0.142 0.081 5.530 10 0.137
14 0.224
2 8 18 0.111
10
14 4 2 0.018
18 4 0.011
6 0.032
9 8 0.015 0.054 0.031 3.245 10 0.179
10 0.028 0.055 0.007 2.474 14 0.263
14 0.017 0.111 0.109 3.601 18 0.162
18 0.042 0.106 0.055 4.263
11 2 0.014
10 8 3.397 4 0.013
10 0.027 0.112 0.008 2.235 6 0.013
14 0.009 0.144 0.052 3.483 10 0.066
18 0.045 0.134 0.042 4.418 14 0.119
18 0.153
5 8 0.090 0.149 0.123 14.254
10 0.197 0.141 0.099 13.339 12 2 0.016
14 0.143 0.181 0.042 13.323 4 0.006
18 0.226 0.168 0.148 12.688 6 0.018
10 0.069
6 8 0.144 0.278 0.190 15.622 14 0.079
10 0.174 0.171 0.087 13.339 18 0.231
14 0.224 0.221 0.038 14.487
18 0.294 0.213 0.140 12.676 7 2 0.147
4 0.087
13 8 0.070 0.138 0.128 15.230 6 0.081
10 0.162 0.252 0.052 13.767 10 0.257
14 0.105 0.215 0.089 11.692 14 0.225
18 0.329 0.225 0.092 12.446 18 0.259

14 8 0.079 0.140 0.179 14.482 8 2 0.119

10 0.197 0.130 0.106 13.767 4 0.084
14 0.163 0.189 0.127 11.631 6 0.094
18 0.286 0.116 0.082 13.296 10 0.265
14 0.267
18 0.150

15 2 0.103
4 0.060
6 0.100
10 0.202
 14 0.155
18 0.145

16 2 0.140
4 0.078
6 0.096
10 0.201
14 0.209
18 0.121
 30

TC Ibs
[protein] [protein] OD 600
0.034 0.000 3.64
0.051 0.000 3.66
0.065 0.000 7.23
0.214 0.095 11.72
0.289 0.000 13.48
0.324 0.047 11.84

0.062 0.000 3.58

0.082 0.000 5.61
0.117 0.000 7.62
0.106 0.107 11.92
0.279 0.052 14.01
0.304 0.000 12.8

0.067 0.000 3.34

0.085 0.000 3.05
0.000 0.000 5.4
0.219 0.000 8.04
0.228 0.000 9.31
0.211 0.000 9.79

0.068 0.000 3.64

0.087 0.000 2.89
0.072 0.000 5.95
0.226 0.000 7.88
0.220 0.000 8.43
0.204 0.000 10.44

0.149 0.000 12.64

0.147 0.000 13.75
0.162 0.000 12.15
0.371 0.000 13.96
0.316 0.049 16.05
0.200 0.000 12.66

0.182 0.000 12.8

0.135 0.000 12.51
0.177 0.000 14.67
0.317 0.050 13.98
0.328 0.049 15.47
0.162 0.000 13.25

0.190 0.000 11.91

0.107 0.000 13.01
0.111 0.000 13.53
0.350 0.000 13.22
0.292 0.000 13.04
0.194 0.000 13.13

0.157 0.000 12.52

0.148 0.000 11.29
0.142 0.000 13.64
0.355 0.047 12.49
0.315 0.000 13.09
0.199 0.000 12.8
 9
f(x) = 1.64x + 0.54
X Y 8
R² = 0.99
7
dcw Av (OD)
6
0.75 1.61
5
1.6 3.29
4
2.65 4.78 3
3.4 6.46 2
4.7 8.03 1

0
0.5 1 1.5 2 2.5 3 3.5 4 4.5 5

30oC 20oC
sample t (induce) OD 600 DCW samplet induce av dcw
3 2 3.64 1.89 1 1 0.21 -0.2
4 3.66 1.9 2 0.51 -0.02
6 7.23 4.08 3 0.65 0.06
10 11.72 6.82 4 0 1.55 0.61
14 13.48 7.9 10 6 2.72 1.33
18 11.84 6.9 12 8 3.84 2.01
14 10 2.47 1.18
4 2 3.58 1.85 18 14 4.64 2.5
4 5.61 3.09 22 18 5.53 3.05
6 7.62 4.32 26 22 5.91 3.28
10 11.92 6.95
14 14.01 8.22 sample t induce av dcw
18 12.8 7.49 9 1 0.12 -0.26
2 0.51 -0.02
11 2 3.34 1.7 3 0.63 0.05
4 3.05 1.53 4 0 1.58 0.63
6 5.4 2.97 10 6 2.21 1.02
10 8.04 4.58 12 8 3.24 1.65
14 9.31 5.35 14 10 2.47 1.18
18 9.79 5.65 18 14 3.6 1.87
22 18 4.26 2.27
12 2 3.64 1.89 26 22 4.71 2.55
4 2.89 1.43
6 5.95 3.3 sample t induce av dcw
10 7.88 4.48 10 1 0.12 -0.26
14 8.43 4.82 2 0.47 -0.04
18 10.44 6.05 3 0.58 0.02
4 0 1.48 0.57
7 2 12.64 7.39 10 6 2.21 1.02
4 13.75 8.07 12 8 3.4 1.74
6 12.15 7.09 14 10 3.34 1.7
10 13.96 8.2 18 14 3.48 1.79
14 16.05 9.47 22 18 4.42 2.37
18 12.66 7.4 26 22 4.29 2.29

8 2 12.8 7.49 sample t induce av OD dcw

4 12.51 7.31 5 1 0.27 -0.17
6 14.67 8.63 2 0.62 0.05
10 13.98 8.2 4 1.86 0.8
 14 15.47 9.12 6 4.95 2.69
18 13.25 7.76 7 0 6.33 3.53
9 2 8.36 4.77
15 2 11.91 6.94 13 6 9.7 5.59
4 13.01 7.61 15 8 14.25 8.37
6 13.53 7.93 17 10 10.92 6.34
10 13.22 7.75 21 14 13.32 7.81
14 13.04 7.63 25 18 12.69 7.42
18 13.13 7.69 29 22 12.15 7.09

16 2 12.52 7.31 sample t induce av dcw

4 11.29 6.56 6 1 0.27 -0.17
6 13.64 8 2 0.62 0.05
10 12.49 7.3 4 2.22 1.02
14 13.09 7.67 6 5.33 2.92
18 12.8 7.49 7 0 7.74 4.4
9 2 10.42 6.03
17 0 4.49 2.41 13 6 11.87 6.92
14 8.69 4.98 15 8 15.62 9.21
18 9.31 5.35 17 10 14.49 8.52
21 14 13.34 7.82
18 0 4.3 2.29 25 18 12.68 7.41
14 11.79 6.87 29 22 12.15 7.09
18 11.03 6.41
sample t induce av dcw
13 1 0.26 -0.18
2 0.61 0.04
4 2.02 0.9
6 5.39 2.96
7 0 6.84 3.85
9 2 7.73 4.39
13 6 9.48 5.46
15 8 15.23 8.97
17 10 9.56 5.51
21 14 11.69 6.81
25 18 12.45 7.27
29 22 13.71 8.04

sample t induce av dcw

14 1 0.26 -0.18
2 0.61 0.04
4 2.69 1.31
6 5.57 3.07
7 0 8.26 4.72
9 2 9.23 5.31
13 6 11.07 6.43
15 8 14.48 8.51
17 10 13.77 8.08
21 14 11.63 6.77
25 18 13.3 7.79
29 22 13.19 7.73
 25oC
sample t induce av OD dcw
23 0 0.2 -0.21
1 0.36 -0.11
3 2.43 1.15
4 3.18 1.61
5 0 3.99 2.1
7 2 5.97 3.31
4 4.5 5 10 5 8.28 4.73
11 6 10.85 6.29
13 8 11.19 6.5
15 10 12.34 7.21
19 14 13.47 7.9
23 18 12.73 7.44
27 22 11.38 6.62
29 24 10.82 6.28

24 0 0.2 -0.21
1 0.37 -0.11
3 1.73 0.72
4 3.38 1.73
5 0 4.11 2.18
7 2 6.13 3.41
10 5 8.55 4.89
11 6 11.32 6.58
13 8 11.34 6.59
15 10 13.26 7.77
19 14 15.41 9.08
23 18 11.75 6.84
27 22 11.33 6.59
29 24 10.91 6.33

sample OD 600 DCW

20 14 13.142 7.695
18 11.798 6.874

21 14 13.840 8.122
18 11.200 6.509

22 14 14.610 8.592
18 11.822 6.889

RSM (inoculum problem) repeat

22 t od DCW 22 t od dcw
0 4.82 2.610 14 13.34 7.816
8 8.83 5.063 18 15.49 9.130
10 17.87 10.586
14 9.63 5.550 25,26,27,28t od dcw
 18 7.77 4.415 14 14.36 8.437
18 15.42 9.088
25 0 4.28 2.279
8 9.57 5.515
10 13.64 8.000
12 16.82 9.942
14 9.47 5.450
16 14.32 8.415
18 7.83 4.450

26 0 4.42 2.365
8 9.43 5.425
10 17.18 10.163
12 17.54 10.384
14 11.05 6.420
16 9.65 5.565
18 7.34 4.154

27 0 4.41 2.359
8 9.01 5.170
10 17.28 10.221
12 12.19 7.111
14 12.43 7.262
16 9.01 5.172
18 7.62 4.321

28 0 4.61 2.484
8 13.93 8.175
10 14.64 8.608
12 10.01 5.783
14 12.95 7.581
16 8.84 5.065
18 9.11 5.234
30oC mg/mL ng/mL
Pr TC Ibs Pr TC Ibs
sample OD 600 DCW Ind hour[protein] [protein] [protein] [IFN] [IFN] [IFN]
3 3.64 1.89 2 0.02 0.03 0 2.31 3.63 1.44
3.66 1.9 4 0 0.05 0 0.68 5.53 0.46
7.23 4.08 6 0.03 0.07 0 5.37 7.59 4.56
11.72 6.82 10 0.14 0.21 0.09 12.73 9.88
13.48 7.9 14 0.22 0.29 0 39.54 12.22 7.15
11.84 6.9 18 0.11 0.32 0.05 13.85 11.16 6.01

4 3.58 1.85 2 0.02 0.06 0 2.93 3.21 1.33

5.61 3.09 4 0.01 0.08 0 2.21 5.16 1.28
7.62 4.32 6 0.03 0.12 0 5.26 6.03 4.11
11.92 6.95 10 0.18 0.11 0.11 22.21 10.71
14.01 8.22 14 0.26 0.28 0.05 60.64 18.85 12.72
12.8 7.49 18 0.16 0.3 0 28.53 14.88 8.87

11 3.34 1.7 2 0.01 0.07 0 2.83 3.76 0.88

3.05 1.53 4 0.01 0.08 0 2.56 11.21 3.15
5.4 2.97 6 0.01 0 0 2.68 0.22 10.77
8.04 4.58 10 0.07 0.22 0 11.82 16.16
9.31 5.35 14 0.12 0.23 0 13.67 20.55 16.43
9.79 5.65 18 0.15 0.21 0 12.43 18.64 8.26

12 3.64 1.89 2 0.02 0.07 0 4.2 5.24 2.18

2.89 1.43 4 0.01 0.09 0 4.52 11.64 7.36
5.95 3.3 6 0.02 0.07 0 3.41 11.63 6.69
7.88 4.48 10 0.07 0.23 0 14.93 18.67
8.43 4.82 14 0.08 0.22 0 14.54 22.38 9.5
10.44 6.05 18 0.23 0.2 0 21.07 21.57 12.07

7 12.64 7.39 2 0.15 0.15 0 12.14 6.03 18.02

13.75 8.07 4 0.09 0.15 0 18.43 10.91 5.93
12.15 7.09 6 0.08 0.16 0 15.33 9.44 4.66
13.96 8.2 10 0.26 0.37 0 43.83 15.14
16.05 9.47 14 0.22 0.32 0.05 29.65 17.63 15.8
12.66 7.4 18 0.26 0.2 0 27.34 15.35 8.55

8 12.8 7.49 2 0.12 0.18 0 17.44 6.84 17.15

12.51 7.31 4 0.08 0.14 0 17.03 12.71 6.49
14.67 8.63 6 0.09 0.18 0 24.44 9.15 5.89
13.98 8.2 10 0.27 0.32 0.05 34.28 15.18
15.47 9.12 14 0.27 0.33 0.05 29.56 16.72 11.21
13.25 7.76 18 0.15 0.16 0 12.85 14.51 7.02

15 11.91 6.94 2 0.1 0.19 0 12.71 7.4 9.93

13.01 7.61 4 0.06 0.11 0 20.42 13.92 5.38
13.53 7.93 6 0.1 0.11 0 17.29 12.07 7.39
13.22 7.75 10 0.2 0.35 0 23.95 20.9
13.04 7.63 14 0.16 0.29 0 27.85 18.76 15.82
13.13 7.69 18 0.14 0.19 0 11.65 20.39 7.65
 16 12.52 7.31 2 0.14 0.16 0 12.73 11.47 6.72
11.29 6.56 4 0.08 0.15 0 17.4 14.87 6.25
13.64 8 6 0.1 0.14 0 16.7 13.73 7.76
12.49 7.3 10 0.2 0.35 0.05 25.39 24.03
13.09 7.67 14 0.21 0.32 0 21.25 33.89 18.67
12.8 7.49 18 0.12 0.2 0 12.7 23.6 9.63

20oC mg/mL ng/mL

Pr TC Ibs Pr TC Ibs
sample OD 600 DCW Ind hour[protein] [protein] [protein] [IFN] [IFN] [IFN]
1 3.84 2.01 8 0.03 0.06 0.04 8.93 2.31 0
2.47 1.18 10 0.04 0.09 0.02 17.7 9.13 3.59
4.64 2.5 14 0.02 0.11 0.1 9.34 3.49 4.82
5.53 3.05 18 0.05 0.14 0.08 7.99 5.43 0

9 3.24 1.65 8 0.02 0.05 0.03 7.59 6.6 5.73

2.47 1.18 10 0.03 0.05 0.01 30.56 15.48 0.46
3.6 1.87 14 0.02 0.11 0.11 9.5 21.81 2.52
4.26 2.27 18 0.04 0.11 0.06 9.6 28.02 2.32

10 3.4 1.74 8
3.34 1.7 10 0.03 0.11 0.01 27.34 11.77 4.3
3.48 1.79 14 0.01 0.14 0.05 12.68 34.42 5.85
4.42 2.37 18 0.05 0.13 0.04 16.64 32.05 0.65

5 14.25 8.37 8 0.09 0.15 0.12 13.33 36.36 11.84

10.92 6.34 10 0.2 0.14 0.1 20.07 15.43 4.82
13.32 7.81 14 0.14 0.18 0.04 59.15 18.45 3.83
12.69 7.42 18 0.23 0.17 0.15 148.29 55.81 17

6 15.62 9.21 8 0.14 0.28 0.19 40.43 25.86 14.62

14.49 8.52 10 0.17 0.17 0.09 31.88 8.08 6.61
13.34 7.82 14 0.22 0.22 0.04 74.05 21.94 14.66
12.68 7.41 18 0.29 0.21 0.14 119.12 44.18 11.64

13 15.23 8.97 8 0.07 0.14 0.13 18.66 19.82

9.56 5.51 10 0.16 0.25 0.05 33.36 23.7 6.53
11.69 6.81 14 0.1 0.21 0.09 56.67 32.13 11.09
12.45 7.27 18 0.33 0.22 0.09 194.04 81.42 21.68

14 14.48 8.51 8 0.08 0.14 0.18 31.31 26.52

13.77 8.08 10 0.2 0.13 0.11 108.6 19.22 6.45
11.63 6.77 14 0.16 0.19 0.13 97.02 36.06 6.96
13.3 7.79 18 0.29 0.12 0.08 244.89 58.08 16.68
25oC mg/mL ng/mL
Pr TC Ibs Pr TC Ibs
sample OD 600 DCW Ind hour[protein] [protein] [protein] [IFN] [IFN] [IFN]
23 11.19 6.5 8 0.19 0.76 0.19 240.08 235.74 190.61
12.34 7.21 10 0.06 0.25 0.11 83.42 97.74 53.58
13.47 7.9 14 0.34 0.41 0.15 361.21 353.76 371.8
12.73 7.44 18 0.34 0.51 0.19 343.02 331.58 371.08

24 11.34 6.59 8 0.18 0.37 0.21 218.05 273.37 164.7

13.26 7.77 10 0.06 0.27 0.11 68.1 91.31 50.25
15.41 9.08 14 0.35 0.52 0.18 340.94 299.32 349.86
11.75 6.84 18 0.34 0.49 0.17 340.62 322.65 389.9

RSM duplicate

25oC mg/mL ng/mL

Pr TC Ibs Pr TC Ibs
sample OD 600 DCW Ind hour[protein] [protein] [protein] [IFN] [IFN] [IFN]
20 13.14 7.9 14 0.34 0.41 0.15 361.21 353.76 371.8
11.8 7.44 18 0.34 0.51 0.19 343.02 331.58 371.08

21 13.84 7.9 14 0.34 0.41 0.15 361.21 353.76 371.8

11.2 7.44 18 0.34 0.51 0.19 343.02 331.58 371.08

(2.66 iptg)22 14.61 7.9 14 0.34 0.41 0.15 361.21 353.76 371.8
11.82 7.44 18 0.34 0.51 0.19 343.02 331.58 371.08

22 13.34 7.82 14 0.43 0.3 0.15 191.37 159.35 -0.58

15.49 9.13 18 0.55 0.33 0.24 191.81 130.98 5.61

25 14.36 8.44 14 0.46 0.31 0.19 180.4 129.07 0.62

15.42 9.09 18 0.44 0.3 0.29 193.42 131.46 6.17

26 14.36 8.44 14 0.46 0.31 0.19 172.78 120.68 4.23

15.42 9.09 18 0.44 0.3 0.29 202.22 175.93 4.98

17 8.69 4.98 14 0.14 0.39 0.2

9.31 5.35 18 0.22 0.33 0.12

18 11.79 6.87 14 0.16 0.44 0.15

11.03 6.41 18 0.24 0.42 0.18
14h

1 0.02 0.11 0.1

2 0.02 0.11 0.1
3 0.22 0.29 0
4 0.26 0.28 0.05
5 0.14 0.18 0.04
6 0.22 0.22 0.04
7 0.22 0.32 0.05
8 0.27 0.33 0.05
9 0.02 0.11 0.11
10 0.01 0.14 0.05
11 0.12 0.23 0
12 0.08 0.22 0
13 0.1 0.21 0.09
14 0.16 0.19 0.13
15 0.16 0.29 0
16 0.21 0.32 0
17 0.14 0.39 0.2
18 0.16 0.44 0.15
19 0 0 0
20 0.34 0.41 0.15
21 0.34 0.41 0.15
22 0.43 0.3 0.15
23 0.34 0.41 0.15
24 0.35 0.52 0.18
25 0.46 0.31 0.19
26 0.46 0.31 0.19
27 0.46 0.31 0.19
28 0.46 0.31 0.19
18h
pr tc ib
1 0.05 0.14 0.08
2 0.05 0.14 0.08
3 0.11 0.32 0.05
4 0.16 0.3 0
5 0.23 0.17 0.15
6 0.29 0.21 0.14
7 0.26 0.2 0
8 0.15 0.16 0
9 0.04 0.11 0.06
10 0.05 0.13 0.04
11 0.15 0.21 0
12 0.23 0.2 0
13 0.33 0.22 0.09
14 0.29 0.12 0.08
15 0.14 0.19 0
16 0.12 0.2 0
17 0.22 0.33 0.12
18 0.24 0.42 0.18
19 0 0 0
20 0.34 0.51 0.19
21 0.34 0.51 0.19
22 0.55 0.33 0.24
23 0.34 0.51 0.19
24 0.34 0.49 0.17
25 0.44 0.3 0.29
26 0.44 0.3 0.29
27 0.44 0.3 0.29
28 0.44 0.3 0.29
rpt (200209)actual [ifn]
26 14h pr 172.78
25 14h pr 180.4
22 14h pr 191.37
26 18h pr 202.22
25 18h pr 193.42
22 18h pr 191.81
26 14h tc 120.68
25 14h tc 129.07
22 14h tc 159.35
26 18h tc 175.93
25 18h tc 131.46
22 18h tc 130.98

/10
actual [ifn]
22 14h ib
25 14h ib
26 14h ib
22 18h ib
25 18h ib
26 18h ib
interferon
% transfer
total soluble
2 hour DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB) Pr/(Pr+TC)*100
1 0.000 0.000 #DIV/0!
2 0.000 0.000 #DIV/0!
3 1.89 2.31 3.63 1.44 5.942 7.377 38.883
4 1.85 2.93 3.21 1.33 6.146 7.476 47.730
5 0.000 0.000 #DIV/0!
6 0.000 0.000 #DIV/0!
7 7.39 12.14 6.03 18.02 18.175 36.194 66.815
8 7.49 17.44 6.84 17.15 24.278 41.424 71.823
9 0.000 0.000 #DIV/0!
10 0.000 0.000 #DIV/0!
11 1.7 2.83 3.76 0.88 6.593 7.472 42.944
12 1.89 4.2 5.24 2.18 9.440 11.615 44.481
13 0.000 0.000 #DIV/0!
14 0.000 0.000 #DIV/0!
15 6.94 12.71 7.4 9.93 20.110 30.035 63.207
16 7.31 12.73 11.47 6.72 24.204 30.929 52.594
17 0.000 0.000 #DIV/0!
18 0.000 0.000 #DIV/0!

interferon
% transfer
total soluble
4 hour DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB) Pr/(Pr+TC)*100
1 0.000 0.000 #DIV/0!
2 0.000 0.000 #DIV/0!
3 1.9 0.68 5.53 0.46 6.211 6.668 10.983
4 3.09 2.21 5.16 1.28 7.365 8.641 29.979
5 0.000 0.000 #DIV/0!
6 0.000 0.000 #DIV/0!
7 8.07 18.43 10.91 5.93 29.339 35.270 62.826
8 7.31 17.03 12.71 6.49 29.739 36.226 57.258
9 0.000 0.000 #DIV/0!
10 0.000 0.000 #DIV/0!
11 1.53 2.56 11.21 3.15 13.775 16.929 18.595
12 1.43 4.52 11.64 7.36 16.157 23.516 27.946
13 0.000 0.000 #DIV/0!
14 0.000 0.000 #DIV/0!
15 7.61 20.42 13.92 5.38 34.344 39.720 59.467
16 6.56 17.4 14.87 6.25 32.270 38.518 53.921
17 0.000 0.000 #DIV/0!
18 0.000 0.000 #DIV/0!
interferon
% transfer
total soluble
6 hour DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB) Pr/(Pr+TC)*100
1 0.000 0.000 #DIV/0!
2 0.000 0.000 #DIV/0!
3 4.08 5.37 7.59 4.56 12.956 17.512 41.455
4 4.32 5.26 6.03 4.11 11.291 15.397 46.581
5 0.000 0.000 #DIV/0!
6 0.000 0.000 #DIV/0!
7 7.09 15.33 9.44 4.66 24.771 29.433 61.905
8 8.63 24.44 9.15 5.89 33.591 39.482 72.766
9 0.000 0.000 #DIV/0!
10 0.000 0.000 #DIV/0!
11 2.97 2.68 0.22 10.77 2.892 13.664 92.495
12 3.3 3.41 11.63 6.69 15.040 21.725 22.660
13 0.000 0.000 #DIV/0!
14 0.000 0.000 #DIV/0!
15 7.93 17.29 12.07 7.39 29.358 36.744 58.889
16 8 16.7 13.73 7.76 30.437 38.193 54.875
17 0.000 0.000 #DIV/0!
18 0.000 0.000 #DIV/0!

interferon
% transfer
total soluble
8 hour DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB) Pr/(Pr+TC)*100
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18

interferon
% transfer
 total soluble
10 hour DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB) Pr/(Pr+TC)*100
1 1.18 17.700 9.130 3.591 26.830 30.421 65.971
2 1.5 21.458 11.798 6.234 33.256 39.490 64.524
3 6.82 12.73 9.88 0.000 22.613 22.613 56.295
4 6.95 22.21 10.71 0.000 32.921 32.921 67.464
5 6.34 20.07 15.43 4.820 35.498 40.318 56.538
6 8.52 31.876 8.078 6.606 39.954 46.560 79.782
7 8.2 43.83 15.14 0.000 58.970 58.970 74.327
8 8.2 34.28 15.18 0.000 49.453 49.453 69.311
9 1.18 30.560 15.484 0.456 46.044 46.500 66.371
10 1.71 27.343 11.772 4.305 39.115 43.420 69.904
11 4.58 11.82 16.16 0.000 27.981 27.981 42.237
12 4.48 14.93 18.67 0.000 33.600 33.600 44.421
13 5.51 33.355 23.698 6.526 57.053 63.579 58.463
14 8.08 108.599 19.223 6.447 127.822 134.269 84.961
15 7.75 23.95 20.9 0.000 44.848 44.848 53.402
16 7.3 25.39 24.03 0.000 49.425 49.425 51.374
17 7.21 83.42 97.74 53.58 181.158 234.737 46.046
18 7.77 68.1 91.31 50.25 159.416 209.662 42.720
19
20
21
22
23
24
25
26
27
28

interferon
% transfer
total soluble
14 hour DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB) Pr/(Pr+TC)*100
1 2.5 9.341 3.492 4.820 12.833 17.653 72.789
2 2.3 7.453 1.564 3.760 9.017 12.777 82.655
3 7.9 39.54 12.22 7.15 51.760 58.908 76.394
4 8.22 60.64 18.85 12.72 79.493 92.208 76.285
5 7.81 59.15 18.45 3.83 77.604 81.433 76.224
6 7.82 74.047 21.940 14.659 95.987 110.646 77.143
7 9.47 29.65 17.63 15.8 47.285 63.082 62.710
8 9.12 29.56 16.72 11.21 46.280 57.488 63.870
9 1.87 9.502 21.810 2.519 31.312 33.831 30.346
10 1.8 12.653 34.425 5.852 47.078 52.930 26.877
11 5.35 13.67 20.55 16.43 34.223 50.655 39.942
12 4.82 14.54 22.38 9.5 36.921 46.423 39.392
13 6.81 56.673 32.127 11.089 88.800 99.889 63.821
14 6.77 97.017 36.062 6.963 133.079 140.042 72.902
15 7.63 27.85 18.76 15.82 46.606 62.429 59.751
 16 7.67 21.25 33.89 18.67 55.137 73.803 38.543
17 7.9 361.21 353.76 371.8 714.966 1086.763 50.521
18 9.08 340.94 299.32 349.86 640.261 990.118 53.250
19
20
21
22
23
24
25
26
27
28

interferon
% transfer
total soluble
18 hour DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB) Pr/(Pr+TC)*100
1 3.05 7.985 5.427 0.000 13.412 13.412 59.536
2 3.25 9.985 7.427 1.078 17.412 18.490 57.346
3 6.9 13.85 11.16 6.01 25.004 31.015 55.376
4 7.49 28.53 14.88 8.87 43.406 52.273 65.721
5 7.42 148.287 55.813 17.000 204.100 221.100 72.654
6 7.41 119.121 44.184 11.644 163.305 174.949 72.944
7 7.4 27.34 15.35 8.55 42.689 51.239 64.035
8 7.76 12.85 14.51 7.02 27.358 34.373 46.973
9 2.27 9.604 28.024 2.321 37.628 39.949 25.524
10 2.37 16.637 32.053 0.655 48.690 49.345 34.169
11 5.65 12.43 18.64 8.26 31.070 39.328 40.014
12 6.05 21.07 21.57 12.07 42.643 54.710 49.422
13 7.27 194.044 81.415 21.682 275.459 297.141 70.444
14 7.79 244.892 58.083 16.683 302.975 319.658 80.829
15 7.69 11.65 20.39 7.65 32.037 39.688 36.366
16 7.49 12.7 23.6 9.63 36.298 45.932 34.994
17 7.44 343.02 331.58 371.08 674.599 1045.681 50.848
18 6.84 340.62 322.65 389.9 663.268 1053.171 51.355

blank 0.37 0.37 0.36

0.1 0.49 0.48 0.49
0.2 0.59 0.64 0.55
0.3 0.67 0.85 0.75
0.4 0.86 0.81 0.85
0.5 0.95 1.01 0.95
% transfer
all protein
(Pr/total)*100
tempt IPTG OD induce
#DIV/0! 20 0.05 1
#DIV/0! 20 0.05 1
31.319 30 0.05 7
39.243 30 0.05 7
#DIV/0! 20 0.05 1
#DIV/0! 20 0.05 1
33.552 30 0.05 7
42.095 30 0.05 7
#DIV/0! 20 2 1
#DIV/0! 20 2 1
37.889 30 2 7
36.149 30 2 7
#DIV/0! 20 2 1
#DIV/0! 20 2 1
42.321 30 2 7
41.159 30 2 7
#DIV/0! 25 1.03 4
#DIV/0! 25 1.03 4

% transfer
all protein
(Pr/total)*100
tempt IPTG OD induce tempt IPTG OD induce
#DIV/0! 20 0.05 1 1 20 0.05 1
#DIV/0! 20 0.05 1 2 20 0.05 1
10.232 30 0.05 1 3 30 0.05 7
25.551 30 0.05 1 4 30 0.05 7
#DIV/0! 20 0.05 7 5 20 0.05 1
#DIV/0! 20 0.05 7 6 20 0.05 1
52.261 30 0.05 7 7 30 0.05 7
47.005 30 0.05 7 8 30 0.05 7
#DIV/0! 20 2 1 9 20 2 1
#DIV/0! 20 2 1 10 20 2 1
15.131 30 2 1 11 30 2 7
19.200 30 2 1 12 30 2 7
#DIV/0! 20 2 7 13 20 2 1
#DIV/0! 20 2 7 14 20 2 1
51.419 30 2 7 15 30 2 7
45.173 30 2 7 16 30 2 7
#DIV/0! 25 1.03 4 25 1.03 4
#DIV/0! 25 1.03 4 25 1.03 4
% transfer
all protein
(Pr/total)*100
tempt IPTG OD induce
#DIV/0! 20 0.05 1
#DIV/0! 20 0.05 1
30.670 30 0.05 1
34.158 30 0.05 1
#DIV/0! 20 0.05 7
#DIV/0! 20 0.05 7
52.100 30 0.05 7
61.908 30 0.05 7
#DIV/0! 20 2 1
#DIV/0! 20 2 1
19.579 30 2 1
15.687 30 2 1
#DIV/0! 20 2 7
#DIV/0! 20 2 7
47.051 30 2 7
43.731 30 2 7
#DIV/0! 25 1.03 4
#DIV/0! 25 1.03 4

% transfer
all protein
(Pr/total)*100
tempt IPTG OD induce
20 0.05 1
20 0.05 1
30 0.05 1
30 0.05 1
20 0.05 7
20 0.05 7
30 0.05 7
30 0.05 7
20 2 1
20 2 1
30 2 1
30 2 1
20 2 7
20 2 7
30 2 7
30 2 7
25 1.03 4
25 1.03 4

% transfer
all protein
(Pr/total)*100
tempt IPTG OD induce
58.183 20 0.05 1
54.338 20 0.05 1
56.295 30 0.05 1
67.464 30 0.05 1
49.779 20 0.05 7
68.462 20 0.05 7
74.327 30 0.05 7
69.311 30 0.05 7
65.720 20 2 1
62.973 20 2 1
42.237 30 2 1
44.421 30 2 1
52.462 20 2 7
80.882 20 2 7
53.402 30 2 7
51.374 30 2 7
35.536 25 1.03 4
32.482 25 1.03 4

% transfer
all protein
(Pr/total)*100
tempt IPTG OD induce
52.915 20 0.05 1
58.331 20 0.05 1
67.125 30 0.05 1
65.766 30 0.05 1
72.640 20 0.05 7
66.922 20 0.05 7
47.006 30 0.05 7
51.418 30 0.05 7
28.087 20 2 1
23.905 20 2 1
26.986 30 2 1
31.329 30 2 1
56.736 20 2 7
69.277 20 2 7
44.607 30 2 7
 28.795 30 2 7
33.237 25 1.03 4
34.434 25 1.03 4

% transfer
all protein
(Pr/total)*100
tempt IPTG OD induce
59.536 20 0.05 1
54.002 20 0.05 1
44.644 30 0.05 1
54.573 30 0.05 1
67.068 20 0.05 7
68.089 20 0.05 7
53.350 30 0.05 7
37.386 30 0.05 7
24.041 20 2 1
33.716 20 2 1
31.611 30 2 1
38.521 30 2 1
65.304 20 2 7
76.611 20 2 7
29.356 30 2 7
27.654 30 2 7
32.804 25 1.03 4
32.342 25 1.03 4
interferon
% transfer % transfer
total soluble all protein
10 hour OD DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100
tempt IPTG
1 2.47 1.179 17.700 9.130 3.591 26.830 30.421 65.971 58.183 20 0.05
2 3 1.500 21.458 11.798 6.234 33.256 39.490 64.524 54.338 20 0.05
3 11.72 6.824 12.730 9.883 0.000 22.613 22.613 56.295 56.295 30 0.05
4 11.92 6.947 22.210 10.711 0.000 32.921 32.921 67.464 67.464 30 0.05
5 10.92 6.337 20.070 15.428 4.820 35.498 40.318 56.538 49.779 20 0.05
6 14.49 8.517 31.876 8.078 6.606 39.954 46.560 79.782 68.462 20 0.05
7 13.96 8.196 43.830 15.139 0.000 58.970 58.970 74.327 74.327 30 0.05
8 13.98 8.204 34.276 15.177 0.000 49.453 49.453 69.311 69.311 30 0.05
9 2.47 1.179 30.560 15.484 0.456 46.044 46.500 66.371 65.720 20 2
10 3.34 1.705 27.343 11.772 4.305 39.115 43.420 69.904 62.973 20 2
11 8.04 4.579 11.818 16.163 0.000 27.981 27.981 42.237 42.237 30 2
12 7.88 4.480 14.925 18.675 0.000 33.600 33.600 44.421 44.421 30 2
13 9.56 5.510 33.355 23.698 6.526 57.053 63.579 58.463 52.462 20 2
14 13.77 8.077 108.599 19.223 6.447 127.822 134.269 84.961 80.882 20 2
15 13.22 7.745 23.950 20.898 0.000 44.848 44.848 53.402 53.402 30 2
16 12.49 7.297 25.392 24.033 0.000 49.425 49.425 51.374 51.374 30 2
17 12.34 7.205 83.416 97.742 53.579 181.158 234.737 46.046 35.536 25 1.03
18 13.26 7.766 68.102 91.314 50.246 159.416 209.662 42.720 32.482 25 1.03
interferon
% transfer % transfer
total soluble all protein
14 hour OD DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100
tempt IPTG
1 4.64 2.500 9.341 3.492 4.820 12.833 17.653 72.789 52.915 20 0.05
2 4.31 2.300 7.453 1.564 3.760 9.017 12.777 82.655 58.331 20 0.05
3 13.49 7.905 39.542 12.218 7.148 51.760 58.908 76.394 67.125 30 0.05
4 14.01 8.224 60.641 18.851 12.715 79.493 92.208 76.285 65.766 30 0.05
5 13.32 7.806 59.153 18.451 3.829 77.604 81.433 76.224 72.640 20 0.05
6 13.34 7.816 74.047 21.940 14.659 95.987 110.646 77.143 66.922 20 0.05
7 16.05 9.470 29.652 17.633 15.797 47.285 63.082 62.710 47.006 30 0.05
8 15.47 9.116 29.559 16.721 11.208 46.280 57.488 63.870 51.418 30 0.05
9 3.6 1.867 9.502 21.810 2.519 31.312 33.831 30.346 28.087 20 2
10 3.48 1.795 12.653 34.425 5.852 47.078 52.930 26.877 23.905 20 2
11 9.31 5.354 13.669 20.554 16.431 34.223 50.655 39.942 26.986 30 2
12 8.43 4.819 14.544 22.377 9.502 36.921 46.423 39.392 31.329 30 2
13 11.69 6.810 56.673 32.127 11.089 88.800 99.889 63.821 56.736 20 2
14 11.63 6.772 97.017 36.062 6.963 133.079 140.042 72.902 69.277 20 2
15 13.04 7.634 27.848 18.758 15.823 46.606 62.429 59.751 44.607 30 2
16 13.09 7.666 21.252 33.885 18.666 55.137 73.803 38.543 28.795 30 2
17
18
19 0 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 25 1.03
20 13.142 7.695 384.980 134.860 90.790 519.840 610.630 74.057 63.046 25 1.03
21 13.840 8.122 0 20.01 45.84 20.010 65.850 0.000 0.000 25 0
22 14.610 8.592 187.84 183.27 220.96 371.110 592.070 50.616 31.726 25 2.66
22 13.34 7.82 191.37 159.35 27.55 350.718 378.271 54.564 50.589 25 2.98
23 13.47 7.896 361.209 353.757 371.797 714.966 1086.763 50.521 33.237 25 1.03
24 15.41 9.080 340.937 299.324 349.857 640.261 990.118 53.250 34.434 25 1.03
25 14.36 8.44 180.4 129.07 39.57 309.468 349.042 58.293 51.683 25 1.03
26 14.36 8.44 172.78 120.68 35.61 293.461 329.068 58.876 52.505 25 1.03
27 12.43 7.26 39.2 36.58 28.74 75.781 104.524 51.731 37.505 25 1.03
28 12.95 7.58 44.78 42.2 36.04 86.986 123.030 51.484 36.401 25 1.03
interferon
% transfer % transfer
total soluble all protein
18 hour OD DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100
tempt IPTG
1 5.53 3.046 7.985 5.427 0.000 13.412 13.412 59.536 59.536 20 0.05
2 5.86 3.246 9.985 7.427 1.078 17.412 18.490 57.346 54.002 20 0.05
3 11.84 6.900 13.846 11.158 6.011 25.004 31.015 55.376 44.644 30 0.05
4 12.81 7.489 28.527 14.879 8.867 43.406 52.273 65.721 54.573 30 0.05
5 12.69 7.418 148.287 55.813 17.000 204.100 221.100 72.654 67.068 20 0.05
6 12.68 7.411 119.121 44.184 11.644 163.305 174.949 72.944 68.089 20 0.05
7 12.66 7.401 27.336 15.353 8.550 42.689 51.239 64.035 53.350 30 0.05
8 13.25 7.758 12.851 14.507 7.016 27.358 34.373 46.973 37.386 30 0.05
9 4.26 2.272 9.604 28.024 2.321 37.628 39.949 25.524 24.041 20 2
10 4.42 2.366 16.637 32.053 0.655 48.690 49.345 34.169 33.716 20 2
11 9.79 5.649 12.432 18.637 8.259 31.070 39.328 40.014 31.611 30 2
12 10.45 6.048 21.075 21.568 12.067 42.643 54.710 49.422 38.521 30 2
13 12.45 7.270 194.044 81.415 21.682 275.459 297.141 70.444 65.304 20 2
14 13.3 7.789 244.892 58.083 16.683 302.975 319.658 80.829 76.611 20 2
15 13.13 7.686 11.651 20.386 7.651 32.037 39.688 36.366 29.356 30 2
16 12.8 7.487 12.702 23.596 9.634 36.298 45.932 34.994 27.654 30 2
17
18
19 0 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 25 1.03
20 11.798 6.874 362.330 112.290 67.270 474.620 541.890 76.341 66.864 25 1.03
21 11.200 6.509 0 16.26 30.21 16.260 46.470 0.000 0.000 25 0
22 11.822 6.889 140.7 116.64 131.22 257.340 388.560 54.675 36.211 25 2.66
22 15.49 9.13 191.81 130.98 54.41 322.794 377.206 59.422 50.851 25 2.98
23 12.73 7.444 343.021 331.578 371.082 674.599 1045.681 50.848 32.804 25 1.03
24 11.75 6.844 340.621 322.647 389.903 663.268 1053.171 51.355 32.342 25 1.03
25 15.42 9.088 193.416 131.457 76.708 324.874 401.582 59.536 48.164 25 1.03
26 15.42 9.088 202.222 175.931 41.082 378.153 419.235 53.476 48.236 25 1.03
27 7.62 4.32 55.47 47.59 40.33 103.062 143.390 53.825 38.687 25 1.03
28 9.11 5.23 40.97 42.93 42.55 83.902 126.452 48.836 32.403 25 1.03
OD induce
1
1
1
1
7
7
7
7
1
1
1
1
7
7
7
7
4
4
OD induce
1
1
1
1
7
7
7
7
1
1
1
1
7
7
7
7
4
4
0
8 14
4 14
4 14
4
4
4
4
4
4
4
13.34 7.82 14
15.49 9.13 18
 14.36 8.44 14
15.42 9.09 18

14.36 8.44 14
OD induce 15.42 9.09 18
1
1
1
1
7
7
7
7
1
1
1
1
7
7
7
7
4
4
0
8 18
4 18
4 18
4
4
4
4
4
4
4
interferon
% transfer % transfer
total soluble all protein
10 hour OD DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 tempt IPTG OD induce
1 2.47 1.179 17.700 9.130 3.591 26.830 30.421 65.971 58.183 20 0.05 1
2 3 1.500 21.458 11.798 6.234 33.256 39.490 64.524 54.338 20 0.05 1
3 11.72 0.582 1.087 0.844 0.000 1.930 1.930 56.295 56.295 30 0.05 7
4 11.92 0.583 22.210 10.711 0.000 32.921 32.921 67.464 67.464 30 0.05 7
5 10.92 6.337 20.070 15.428 4.820 35.498 40.318 56.538 49.779 20 0.05 1
6 14.49 8.517 31.876 8.078 6.606 39.954 46.560 79.782 68.462 20 0.05 1
7 13.96 8.196 43.830 15.139 0.000 58.970 58.970 74.327 74.327 30 0.05 7
8 13.98 8.204 34.276 15.177 0.000 49.453 49.453 69.311 69.311 30 0.05 7
9 2.47 1.179 30.560 15.484 0.456 46.044 46.500 66.371 65.720 20 2 1
10 3.34 1.705 27.343 11.772 4.305 39.115 43.420 69.904 62.973 20 2 1
11 8.04 4.579 11.818 16.163 0.000 27.981 27.981 42.237 42.237 30 2 7
12 7.88 4.480 14.925 18.675 0.000 33.600 33.600 44.421 44.421 30 2 7
13 9.56 5.510 33.355 23.698 6.526 57.053 63.579 58.463 52.462 20 2 1
14 13.77 8.077 108.599 19.223 6.447 127.822 134.269 84.961 80.882 20 2 1
15 13.22 7.745 23.950 20.898 0.000 44.848 44.848 53.402 53.402 30 2 7
16 12.49 7.297 25.392 24.033 0.000 49.425 49.425 51.374 51.374 30 2 7
17 12.34 7.205 83.416 97.742 53.579 181.158 234.737 46.046 35.536 25 1.03 4
18 13.26 7.766 68.102 91.314 50.246 159.416 209.662 42.720 32.482 25 1.03 4
19
20
21
22
23
24
25
26
27
28

10 hour OD DCW (g/L) Pr/OD TC/OD IB/OD Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100

 1 2.47 1.179 7.154 3.690 1.451 10.845 12.296 65.971 58.183
2 3 1.500 7.152 3.932 2.078 11.085 13.162 64.524 54.338
3 11.72 0.582 0.093 0.072 0.000 0.165 0.165 56.295 56.295
4 11.92 0.583 1.864 0.899 0.000 2.763 2.763 67.464 67.464
5 10.92 6.337 1.838 1.413 0.441 3.251 3.693 56.538 49.779
6 14.49 8.517 2.200 0.558 0.456 2.758 3.214 79.782 68.462
7 13.96 8.196 3.139 1.084 0.000 4.224 4.224 74.327 74.327
8 13.98 8.204 2.453 1.086 0.000 3.539 3.539 69.311 69.311
9 2.47 1.179 12.352 6.259 0.184 18.611 18.795 66.371 65.720
10 3.34 1.705 8.199 3.530 1.291 11.729 13.019 69.904 62.973
11 8.04 4.579 1.470 2.010 0.000 3.480 3.480 42.237 42.237
12 7.88 4.480 1.894 2.370 0.000 4.265 4.265 44.421 44.421
13 9.56 5.510 3.488 2.478 0.682 5.965 6.648 58.463 52.462
14 13.77 8.077 7.888 1.396 0.468 9.285 9.753 84.961 80.882
15 13.22 7.745 1.811 1.580 0.000 3.391 3.391 53.402 53.402
16 12.49 7.297 2.033 1.924 0.000 3.957 3.957 51.374 51.374
17 12.34 7.205 6.760 7.921 4.342 14.681 19.022 46.046 35.536
18 13.26 7.766 5.137 6.887 3.790 12.024 15.814 42.720 32.482
19
20
21
22
23
24
25
26
27
28

interferon
% transfer % transfer
total soluble all protein
14 hour OD DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 tempt IPTG OD induce
1 4.64 2.500 9.341 3.492 4.820 12.833 17.653 72.789 52.915 20 0.05 1
 2 4.31 2.300 7.453 1.564 3.760 9.017 12.777 82.655 58.331 20 0.05 1
3 13.49 7.905 59.542 12.218 17.148 71.760 88.908 82.974 66.970 30 0.05 7
4 14.01 8.224 60.641 18.851 12.715 79.493 92.208 76.285 65.766 30 0.05 7
5 13.32 7.806 69.153 18.451 3.829 87.604 91.433 78.938 75.632 20 0.05 1
6 13.34 7.816 74.047 21.940 14.659 95.987 110.646 77.143 66.922 20 0.05 1
7 16.05 9.470 29.652 17.633 15.797 47.285 63.082 62.710 47.006 30 0.05 7
8 15.47 9.116 29.559 16.721 11.208 46.280 57.488 63.870 51.418 30 0.05 7
9 3.6 1.867 9.502 21.810 2.519 31.312 33.831 30.346 28.087 20 2 1
10 3.48 1.795 12.653 24.425 5.852 37.078 42.930 34.125 29.474 20 2 1
11 9.31 5.354 13.669 20.554 16.431 34.223 50.655 39.942 26.986 30 2 7
12 8.43 4.819 14.544 22.377 9.502 36.921 46.423 39.392 31.329 30 2 7
13 11.69 6.810 86.673 32.127 11.089 118.800 129.889 72.957 66.729 20 2 1
14 11.63 6.772 97.017 36.062 6.963 133.079 140.042 72.902 69.277 20 2 1
15 13.04 7.634 27.848 28.758 15.823 56.606 72.429 49.196 38.448 30 2 7
16 13.09 7.665 21.252 33.885 18.666 55.137 73.803 38.543 28.795 30 2 7
17 8.69 4.976 25.96 56.7 52.94 82.660 135.604 31.404 19.143 15 1.03 4
18 11.79 6.870 10.14 5.75 5.06 15.891 20.954 63.800 48.384 35 1.03 4
19 0 -0.333 0.000 0.000 0.000 0.000 0.000 0.000 0.000 25 1.03 0
20 13.142 7.695 184.980 134.860 90.790 319.840 410.630 57.835 45.048 25 1.03 8
21 13.840 8.122 0 20.01 45.84 20.010 65.850 0.000 0.000 25 0 4
22 13.340 7.816 191.37 159.35 189.36 350.718 540.078 54.564 35.433 25 2.98 4
23 13.47 7.896 361.209 353.757 371.797 714.966 1086.763 50.521 33.237 25 1.03 4
24 15.41 9.080 394.937 365.324 389.857 760.261 1150.118 51.948 34.339 25 1.03 4
25 14.36 8.437 380.4 339.07 345.574 719.468 1065.042 52.872 35.717 25 1.03 4
26 14.25 8.372 307.421 314.802 289.607 622.223 911.830 49.407 33.715 25 1.03 4
27 12.43 7.262 316.202 322.579 316.743 638.781 955.524 49.501 33.092 25 1.03 4
28 12.95 7.581 334.784 312.202 348.043 646.986 995.030 51.745 33.646 25 1.03 4

total % soluble
14 hour OD DCW (g/L) Pr/OD TC/OD IB/OD Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100
1 4.637 2.500 2.014 0.753 1.039 2.768 3.807 72.789 52.915
2 4.310 2.300 1.729 0.363 0.872 2.092 2.965 82.655 58.331
3 13.485 7.905 4.415 0.906 1.272 5.321 6.593 82.974 66.970
4 14.007 8.224 4.329 1.346 0.908 5.675 6.583 76.285 65.766
5 13.323 7.806 5.190 1.385 0.287 6.575 6.863 78.938 75.632
 6 13.339 7.816 5.551 1.645 1.099 7.196 8.295 77.143 66.922
7 16.047 9.470 1.848 1.099 0.984 2.947 3.931 62.710 47.006
8 15.468 9.116 1.911 1.081 0.725 2.992 3.717 63.870 51.418
9 3.601 1.867 2.639 6.057 0.700 8.695 9.395 30.346 28.087
10 3.483 1.795 3.633 7.013 1.680 10.645 12.326 34.125 29.474
11 9.309 5.354 1.468 2.208 1.765 3.676 5.441 39.942 26.986
12 8.433 4.819 1.725 2.654 1.127 4.378 5.505 39.392 31.329
13 11.692 6.810 7.413 2.748 0.948 10.161 11.109 72.957 66.729
14 11.631 6.772 8.341 3.101 0.599 11.442 12.040 72.902 69.277
15 13.041 7.634 2.135 2.205 1.213 4.341 5.554 49.196 38.448
16 13.093 7.666 1.623 2.588 1.426 4.211 5.637 38.543 28.795
17 8.691 4.976 2.987 6.524 6.092 9.511 15.603 31.404 19.143
18 11.792 6.870 0.860 0.488 0.429 1.348 1.777 63.800 48.384
19 0.000 0.000 0.000 0.000 0.000 0.000 0.000 1.000 2.000
20 13.142 7.695 14.075 10.262 6.908 24.337 31.246 57.835 45.048
21 13.840 8.122 0.000 1.446 3.312 1.446 4.758 0.000 0.000
22 14.610 8.592 #REF! #REF! 14.195 #REF! #REF! #REF! #REF!
23 13.470 7.896 26.816 26.263 27.602 53.078 80.680 50.521 33.237
24 15.409 9.080 25.630 23.708 25.301 49.339 74.639 51.948 34.339
25 9.466 5.450 #REF! #REF! 36.507 #REF! #REF! #REF! #REF!
26 11.054 6.420 27.811 28.479 26.199 56.289 82.489 49.407 33.715
27 12.432 7.262 25.435 25.947 25.478 51.382 76.860 49.501 33.092
28 12.954 7.581 25.844 24.101 26.868 49.945 76.813 51.745 33.646

interferon
% transfer % transfer
total soluble all protein
18 hour OD DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 tempt IPTG OD induce
1 5.53 3.045 7.985 5.427 0.000 13.412 13.412 59.536 59.536 20 0.05 1
2 5.86 3.246 9.985 7.427 1.078 17.412 18.490 57.346 54.002 20 0.05 1
3 11.84 6.899 13.846 11.158 6.011 25.004 31.015 55.376 44.644 30 0.05 7
4 12.81 7.489 28.527 14.879 8.867 43.406 52.273 65.721 54.573 30 0.05 7
5 12.69 7.418 148.287 55.813 17.000 204.100 221.100 72.654 67.068 20 0.05 1
6 12.68 7.411 119.121 44.184 11.644 163.305 174.949 72.944 68.089 20 0.05 1
7 12.66 7.402 27.336 15.353 8.550 42.689 51.239 64.035 53.350 30 0.05 7
 8 13.25 7.758 12.851 14.507 7.016 27.358 34.373 46.973 37.386 30 0.05 7
9 4.26 2.271 9.604 28.024 2.321 37.628 39.949 25.524 24.041 20 2 1
10 4.42 2.366 16.637 32.053 0.655 48.690 49.345 34.169 33.716 20 2 1
11 9.79 5.650 12.432 18.637 8.259 31.070 39.328 40.014 31.611 30 2 7
12 10.45 6.048 21.075 21.568 12.067 42.643 54.710 49.422 38.521 30 2 7
13 12.45 7.270 194.044 81.415 21.682 275.459 297.141 70.444 65.304 20 2 1
14 13.3 7.789 244.892 58.083 16.683 302.975 319.658 80.829 76.611 20 2 1
15 13.13 7.686 11.651 20.386 7.651 32.037 39.688 36.366 29.356 30 2 7
16 12.8 7.487 12.702 23.596 9.634 36.298 45.932 34.994 27.654 30 2 7
17 9.31 5.351 136.66 95.94 88.41 232.605 321.016 58.753 42.572 15 1.03 4
18 11.03 6.406 3.69 13.99 13.75 17.673 31.420 20.863 11.735 35 1.03 4
19 0 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 25 1.03 0
20 11.798 6.874 362.330 112.290 67.270 474.620 541.890 76.341 66.864 25 1.03 8
21 11.200 6.509 0 16.26 30.21 16.260 46.470 0.000 0.000 25 0 4
22 15.490 9.130 191.81 130.98 131.22 322.794 454.014 59.422 42.248 25 2.98 4
23 12.73 7.444 343.021 331.578 371.082 674.599 1045.681 50.848 32.804 25 1.03 4
24 11.75 6.844 340.621 322.647 309.903 663.268 973.171 51.355 35.001 25 1.03 4
25 15.42 9.088 193.42 131.46 260.71 324.874 585.582 59.536 33.030 25 1.03 4
26 15.42 9.088 210.32 207.7 210.08 418.020 628.102 50.313 33.485 25 1.03 4
27 7.62 4.321 205.47 203.59 230.33 409.062 639.390 50.230 32.136 25 1.03 4
28 9.11 5.234 245.97 235.93 252.55 481.902 734.452 51.042 33.491 25 1.03 4

total % transfer soluble

18 hour OD DCW (g/L) Pr/OD TC/OD IB/OD Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100
1 5.530 3.045 1.444 0.981 0.000 2.425 2.425 59.536 59.536
2 5.858 3.246 1.705 1.268 0.184 2.972 3.156 57.346 54.002
3 11.839 6.899 1.170 0.942 0.508 2.112 2.620 55.376 44.644
4 12.805 7.489 2.228 1.162 0.692 3.390 4.082 65.721 54.573
5 12.688 7.418 11.687 4.399 1.340 16.086 17.426 72.654 67.068
6 12.676 7.411 9.397 3.486 0.919 12.883 13.802 72.944 68.089
7 12.661 7.402 2.159 1.213 0.675 3.372 4.047 64.035 53.350
8 13.245 7.758 0.970 1.095 0.530 2.066 2.595 46.973 37.386
9 4.263 2.271 2.253 6.574 0.544 8.827 9.371 25.524 24.041
10 4.418 2.366 3.766 7.255 0.148 11.021 11.169 34.169 33.716
11 9.793 5.650 1.269 1.903 0.843 3.173 4.016 40.014 31.611
12 10.445 6.048 2.018 2.065 1.155 4.083 5.238 49.422 38.521
 13 12.446 7.270 15.591 6.541 1.742 22.132 23.874 70.444 65.304
14 13.296 7.789 18.418 4.368 1.255 22.787 24.042 80.829 76.611
15 13.127 7.686 0.888 1.553 0.583 2.441 3.023 36.366 29.356
16 12.801 7.487 0.992 1.843 0.753 2.836 3.588 34.994 27.654
17 9.305 5.351 14.687 10.311 9.502 24.998 34.499 58.753 42.572
18 11.032 6.406 0.334 1.268 1.246 1.602 2.848 20.863 11.735
19 0.000 -0.333 0.000 0.000 0.000 0.000 0.000 0.000 0.000
20 11.798 6.874 30.711 9.518 5.702 40.229 45.931 76.341 66.864
21 11.200 6.509 0.000 1.452 2.697 1.452 4.149 0.000 0.000
22 11.822 6.889 12.383 8.456 8.471 20.839 29.310 59.422 42.248
23 12.731 7.444 26.944 26.045 29.148 52.989 82.137 50.848 32.804
24 11.748 6.844 28.994 27.464 26.379 56.458 82.837 51.355 35.001
25 7.830 4.450 12.542 8.524 33.296 21.066 54.362 59.536 23.071
26 7.344 4.154 13.638 13.468 28.606 27.105 55.711 50.313 24.479
27 7.618 4.321 26.972 26.725 30.235 53.697 83.932 50.230 32.136
28 9.112 5.234 26.995 25.892 27.716 52.887 80.603 51.042 33.491

Specific ifn production (ng/mL/OD)

% transfer % transfer
total soluble all protein
10 hour OD DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 tempt IPTG OD induce
1 2.47 1.18 7.15 3.69 1.45 10.84 12.30 65.97 58.18 20 0.05 1
2 3.00 1.50 7.15 3.93 2.08 11.08 13.16 64.52 54.34 20 0.05 1
3 11.72 0.58 0.09 0.07 0.00 0.16 0.16 56.29 56.29 30 0.05 7
 4 11.92 0.58 1.86 0.90 0.00 2.76 2.76 67.46 67.46 30 0.05 7
5 10.92 6.34 1.84 1.41 0.44 3.25 3.69 56.54 49.78 20 0.05 1
6 14.49 8.52 2.20 0.56 0.46 2.76 3.21 79.78 68.46 20 0.05 1
7 13.96 8.20 3.14 1.08 0.00 4.22 4.22 74.33 74.33 30 0.05 7
8 13.98 8.20 2.45 1.09 0.00 3.54 3.54 69.31 69.31 30 0.05 7
9 2.47 1.18 12.35 6.26 0.18 18.61 18.80 66.37 65.72 20 2 1
10 3.34 1.71 8.20 3.53 1.29 11.73 13.02 69.90 62.97 20 2 1
11 8.04 4.58 1.47 2.01 0.00 3.48 3.48 42.24 42.24 30 2 7
12 7.88 4.48 1.89 2.37 0.00 4.26 4.26 44.42 44.42 30 2 7
13 9.56 5.51 3.49 2.48 0.68 5.97 6.65 58.46 52.46 20 2 1
14 13.77 8.08 7.89 1.40 0.47 9.28 9.75 84.96 80.88 20 2 1
15 13.22 7.75 1.81 1.58 0.00 3.39 3.39 53.40 53.40 30 2 7
16 12.49 7.30 2.03 1.92 0.00 3.96 3.96 51.37 51.37 30 2 7
17 12.34 7.21 6.76 7.92 4.34 14.68 19.02 46.05 35.54 25 1.03 4
18 13.26 7.77 5.14 6.89 3.79 12.02 15.81 42.72 32.48 25 1.03 4

Specific ifn production (ng/mL/OD)

% transfer % transfer
total soluble all protein
14 hour OD DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 tempt IPTG OD induce
1 4.64 2.50 2.01 0.75 1.04 2.77 3.81 72.79 52.91 20 0.05 1
2 4.31 2.30 1.73 0.36 0.87 2.09 2.96 82.65 58.33 20 0.05 1
3 13.49 7.90 2.93 0.91 0.53 3.84 4.37 76.39 67.12 30 0.05 7
4 14.01 8.22 4.33 1.35 0.91 5.68 6.58 76.29 65.77 30 0.05 7
5 13.32 7.81 4.44 1.38 0.29 5.82 6.11 76.22 72.64 20 0.05 1
6 13.34 7.82 5.55 1.64 1.10 7.20 8.29 77.14 66.92 20 0.05 1
7 16.05 9.47 1.85 1.10 0.98 2.95 3.93 62.71 47.01 30 0.05 7
8 15.47 9.12 1.91 1.08 0.72 2.99 3.72 63.87 51.42 30 0.05 7
9 3.60 1.87 2.64 6.06 0.70 8.70 9.39 30.35 28.09 20 2 1
10 3.48 1.80 3.63 9.88 1.68 13.52 15.20 26.88 23.91 20 2 1
11 9.31 5.35 1.47 2.21 1.77 3.68 5.44 39.94 26.99 30 2 7
12 8.43 4.82 1.72 2.65 1.13 4.38 5.50 39.39 31.33 30 2 7
13 11.69 6.81 4.85 2.75 0.95 7.59 8.54 63.82 56.74 20 2 1
14 11.63 6.77 8.34 3.10 0.60 11.44 12.04 72.90 69.28 20 2 1
15 13.04 7.63 2.14 1.44 1.21 3.57 4.79 59.75 44.61 30 2 7
 16 13.09 7.67 1.62 2.59 1.43 4.21 5.64 38.54 28.79 30 2 7
17 13.47 7.90 26.82 26.26 27.60 53.08 80.68 50.52 33.24 25 1.03 4
18 15.41 9.08 22.13 19.43 22.70 41.55 64.26 53.25 34.43 25 1.03 4

Specific ifn production (ng/mL/OD)

% transfer % transfer
total soluble all protein
18 hour OD DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 tempt IPTG OD induce
1 5.53 3.05 1.44 0.98 0.00 2.43 2.43 59.54 59.54 20 0.05 1
2 5.86 3.25 1.70 1.27 0.18 2.97 3.16 57.35 54.00 20 0.05 1
3 11.84 6.90 1.17 0.94 0.51 2.11 2.62 55.38 44.64 30 0.05 7
4 12.81 7.49 2.23 1.16 0.69 3.39 4.08 65.72 54.57 30 0.05 7
5 12.69 7.42 11.69 4.40 1.34 16.09 17.43 72.65 67.07 20 0.05 1
6 12.68 7.41 9.40 3.49 0.92 12.88 13.80 72.94 68.09 20 0.05 1
7 12.66 7.40 2.16 1.21 0.68 3.37 4.05 64.03 53.35 30 0.05 7
8 13.25 7.76 0.97 1.10 0.53 2.07 2.60 46.97 37.39 30 0.05 7
9 4.26 2.27 2.25 6.57 0.54 8.83 9.37 25.52 24.04 20 2 1
10 4.42 2.37 3.77 7.26 0.15 11.02 11.17 34.17 33.72 20 2 1
11 9.79 5.65 1.27 1.90 0.84 3.17 4.02 40.01 31.61 30 2 7
12 10.45 6.05 2.02 2.06 1.16 4.08 5.24 49.42 38.52 30 2 7
13 12.45 7.27 15.59 6.54 1.74 22.13 23.87 70.44 65.30 20 2 1
14 13.30 7.79 18.42 4.37 1.25 22.79 24.04 80.83 76.61 20 2 1
15 13.13 7.69 0.89 1.55 0.58 2.44 3.02 36.37 29.36 30 2 7
16 12.80 7.49 0.99 1.84 0.75 2.84 3.59 34.99 27.65 30 2 7
17 12.73 7.44 26.94 26.04 29.15 52.99 82.14 50.85 32.80 25 1.03 4
18 11.75 6.84 28.99 27.46 41.70 56.46 98.16 51.35 29.54 25 1.03 4
OD induce
 Protein % transfer % transfer
total soluble all protein
OD induce 14 hour OD DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100
1 4.64 2.500 0.02 0.11 0.1 0.127 0.232 13.928 7.631
 2 4.31 2.300 0.02 0.11 0.1 0.127 0.232 13.928 7.631
3 13.49 7.905 0.22 0.29 0 0.513 0.513 43.657 43.657
4 14.01 8.224 0.26 0.28 0.05 0.542 0.594 48.463 44.236
5 13.32 7.806 0.14 0.18 0.04 0.324 0.366 44.129 39.104
6 13.34 7.816 0.22 0.22 0.04 0.445 0.483 50.292 46.315
7 16.05 9.470 0.22 0.32 0.05 0.541 0.589 41.568 38.138
8 15.47 9.116 0.27 0.33 0.05 0.595 0.645 44.868 41.444
9 3.6 1.867 0.02 0.11 0.11 0.127 0.236 12.983 6.999
10 3.48 1.795 0.01 0.14 0.05 0.154 0.205 6.050 4.523
11 9.31 5.354 0.12 0.23 0 0.347 0.347 34.334 34.334
12 8.43 4.819 0.08 0.22 0 0.300 0.300 26.459 26.459
13 11.69 6.810 0.1 0.21 0.09 0.319 0.409 32.779 25.612
14 11.63 6.772 0.16 0.19 0.13 0.353 0.480 46.316 34.061
15 13.04 7.634 0.16 0.29 0 0.447 0.447 34.715 34.715
16 13.09 7.666 0.21 0.32 0 0.524 0.524 39.842 39.842
17 8.69 4.98 0.14 0.39 0.2 0.533 0.730 26.816 19.593
18 11.79 6.87 0.16 0.44 0.15 0.596 0.746 26.349 21.038
19 0 0 0 0 0 0.000 0.000 0.000 0.000
20 13.142 7.695 0.34 0.41 0.15 0.742 0.891 45.195 37.669
21 13.840 8.122 0.34 0.41 0.15 0.742 0.891 45.195 37.669
22 13.34 7.82 0.43 0.3 0.15 0.726 0.875 58.854 48.846
23 13.47 7.896 0.34 0.41 0.15 0.742 0.891 45.195 37.669
24 15.41 9.080 0.35 0.52 0.18 0.865 1.050 39.973 32.930
25 9.47 5.450 0.46 0.31 0.19 0.774 0.968 59.478 47.552
26 11.05 6.420 0.46 0.31 0.19 0.774 0.968 59.478 47.552
27 12.43 7.262 0.46 0.31 0.19 0.774 0.968 59.478 47.552
28 12.95 7.581 0.46 0.31 0.19 0.774 0.968 59.478 47.552

microg/mL
14 hour OD DCW (g/L) Pr/OD TC/OD IB/OD Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100
(Pr/total)*100
1 4.64 2.500 3.820 23.607 22.632 27.427 50.059 13.928 7.631
2 4.31 2.300 4.110 25.399 24.350 29.510 53.860 13.928 7.631
3 13.49 7.905 16.601 21.425 0.000 38.026 38.026 43.657 43.657
4 14.01 8.224 18.759 19.949 3.699 38.707 42.406 48.463 44.236
5 13.32 7.806 10.745 13.604 3.129 24.349 27.478 44.129 39.104
 6 13.34 7.816 16.785 16.590 2.866 33.376 36.242 50.292 46.315
7 16.05 9.470 14.008 19.692 3.030 33.700 36.730 41.568 38.138
8 15.47 9.116 17.271 21.222 3.181 38.493 41.674 44.868 41.444
9 3.6 1.867 4.589 30.759 30.222 35.348 65.570 12.983 6.999
10 3.48 1.795 2.668 41.439 14.882 44.108 58.990 6.050 4.523
11 9.31 5.354 12.795 24.471 0.000 37.266 37.266 34.334 34.334
12 8.43 4.819 9.403 26.135 0.000 35.537 35.537 26.459 26.459
13 11.69 6.810 8.957 18.369 7.646 27.326 34.972 32.779 25.612
14 11.63 6.772 14.053 16.289 10.916 30.342 41.258 46.316 34.061
15 13.04 7.634 11.894 22.367 0.000 34.261 34.261 34.715 34.715
16 13.09 7.666 15.949 24.082 0.000 40.031 40.031 39.842 39.842
17 8.69 4.98 16.448 44.888 22.610 61.336 83.946 26.816 19.593
18 11.79 6.87 13.311 37.208 12.752 50.519 63.271 26.349 21.038
19 0 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
20 13.142 7.695 25.533 30.963 11.287 56.496 67.783 45.195 37.669
21 13.840 8.122 24.246 29.401 10.718 53.647 64.365 45.195 37.669
22 13.34 7.82 32.024 22.389 11.148 54.414 65.562 58.854 48.846
23 13.47 7.896 24.912 30.209 11.012 55.120 66.133 45.195 37.669
24 15.41 9.080 22.440 33.698 12.006 56.137 68.143 39.973 32.930
25 9.47 5.450 48.612 33.119 20.498 81.731 102.229 59.478 47.552
26 11.05 6.420 41.629 28.361 17.553 69.990 87.543 59.478 47.552
27 12.43 7.262 37.014 25.218 15.608 62.232 77.840 59.478 47.552
28 12.95 7.581 35.523 24.201 14.979 59.724 74.703 59.478 47.552

protein % transfer % transfer

total soluble all protein
OD induce 18 hour OD DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100
1 5.530 3.046 0.045 0.142 0.081 0.187 0.268 24.216 16.879
2 5.858 3.246 0.045 0.142 0.081 0.187 0.268 24.216 16.879
3 11.839 6.900 0.111 0.324 0.047 0.435 0.482 25.588 23.094
4 12.805 7.489 0.162 0.304 0.000 0.466 0.466 34.825 34.825
5 12.688 7.418 0.226 0.168 0.148 0.394 0.542 57.371 41.722
6 12.676 7.411 0.294 0.213 0.140 0.507 0.647 58.049 45.486
7 12.661 7.401 0.259 0.200 0.000 0.459 0.459 56.416 56.416
 8 13.245 7.758 0.150 0.162 0.000 0.312 0.312 47.943 47.943
9 4.263 2.272 0.042 0.106 0.055 0.149 0.204 28.523 20.779
10 4.418 2.366 0.045 0.134 0.042 0.179 0.221 25.199 20.392
11 9.793 5.649 0.153 0.211 0.000 0.364 0.364 42.124 42.124
12 10.445 6.048 0.231 0.204 0.000 0.435 0.435 53.036 53.036
13 12.446 7.270 0.329 0.225 0.092 0.554 0.646 59.441 50.981
14 13.296 7.789 0.286 0.116 0.082 0.401 0.483 71.194 59.176
15 13.127 7.686 0.145 0.194 0.000 0.339 0.339 42.694 42.694
16 12.801 7.487 0.121 0.199 0.000 0.320 0.320 37.761 37.761
17 9.305 5.351 0.222 0.326 0.124 0.548 0.672 40.526 33.067
18 11.032 6.406 0.243 0.416 0.179 0.659 0.838 36.813 28.965
19 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
20 11.798 6.874 0.341 0.511 0.187 0.852 1.039 40.052 32.842
21 11.200 6.509 0.341 0.511 0.187 0.852 1.039 40.052 32.842
22 15.490 9.130 0.550 0.328 0.242 0.877 1.119 62.676 49.135
23 12.731 7.444 0.341 0.511 0.187 0.852 1.039 40.052 32.842
24 11.748 6.844 0.343 0.494 0.169 0.836 1.005 40.992 34.102
25 7.830 4.450 0.441 0.299 0.286 0.740 1.026 59.633 42.995
26 7.344 4.154 0.441 0.299 0.286 0.740 1.026 59.633 42.995
27 7.618 4.321 0.441 0.299 0.286 0.740 1.026 59.633 42.995
28 9.112 5.234 0.441 0.299 0.286 0.740 1.026 59.633 42.995

microg/mL
18 hour OD DCW (g/L) Pr/OD TC/OD IB/OD Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100
(Pr/total)*100
1 5.53 3.046 8.181 25.602 0.015 33.783 33.798 24.216 24.205
2 5.86 3.246 7.723 24.169 0.014 31.891 31.905 24.216 24.205
3 11.84 6.900 9.407 27.356 0.004 36.763 36.766 25.588 25.585
4 12.81 7.489 12.674 23.719 0.000 36.393 36.393 34.825 34.825
5 12.69 7.418 17.817 13.239 0.012 31.055 31.067 57.371 57.349
6 12.68 7.411 23.232 16.790 0.011 40.022 40.033 58.049 58.033
7 12.66 7.401 20.461 15.807 0.000 36.267 36.267 56.416 56.416
8 13.25 7.758 11.292 12.261 0.000 23.553 23.553 47.943 47.943
9 4.26 2.272 9.954 24.944 0.013 34.898 34.911 28.523 28.512
10 4.42 2.366 10.215 30.324 0.010 40.539 40.549 25.199 25.193
11 9.79 5.649 15.650 21.502 0.000 37.153 37.153 42.124 42.124
12 10.45 6.048 22.076 19.548 0.000 41.623 41.623 53.036 53.036
 13 12.45 7.270 26.472 18.062 0.007 44.534 44.542 59.441 59.432
14 13.3 7.789 21.492 8.696 0.006 30.187 30.193 71.194 71.179
15 13.13 7.686 11.023 14.796 0.000 25.819 25.819 42.694 42.694
16 12.8 7.487 9.441 15.561 0.000 25.003 25.003 37.761 37.761
17 9.31 5.35 23.863 35.020 0.013 58.884 58.897 40.526 40.517
18 11.03 6.41 21.995 37.753 0.016 59.748 59.764 36.813 36.803
19 0 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
20 11.798 6.874 28.930 43.301 0.016 72.231 72.247 40.052 40.043
21 11.200 6.509 30.475 45.613 0.017 76.088 76.105 40.052 40.043
22 15.49 9.13 35.505 21.143 0.016 56.648 56.664 62.676 62.659
23 12.73 7.444 26.810 40.128 0.015 66.938 66.953 40.052 40.043
24 11.75 6.844 29.187 42.014 0.014 71.202 71.216 40.992 40.984
25 7.83 4.45 56.349 38.145 0.037 94.494 94.530 59.633 59.609
26 7.34 4.15 60.078 40.669 0.039 100.747 100.786 59.633 59.609
27 7.62 4.32 57.917 39.206 0.038 97.123 97.161 59.633 59.609
28 9.11 5.23 48.421 32.778 0.031 81.199 81.231 59.633 59.609

OD induce
OD induce
OD induce
tempt IPTG OD induce
20 0.05 1
 20 0.05 1
30 0.05 7
30 0.05 7
20 0.05 1
20 0.05 1
30 0.05 7
30 0.05 7
20 2 1
20 2 1
30 2 7
30 2 7
20 2 1
20 2 1
30 2 7
30 2 7
15 1.03 4
35 1.03 4
25 1.03 0
25 1.03 8
25 0 4
25 2.98 4
25 1.03 4
25 1.03 4
25 1.03 4
25 1.03 4
25 1.03 4
25 1.03 4

Pr/total)*100
tempt IPTG OD induce
20 0.05 1
20 0.05 1
30 0.05 7
30 0.05 7
20 0.05 1
20 0.05 1
30 0.05 7
 30 0.05 7
20 2 1
20 2 1
30 2 7
30 2 7
20 2 1
20 2 1
30 2 7
30 2 7
15 1.03 4
35 1.03 4
25 1.03 0
25 1.03 8
25 0 4
25 2.98 4
25 1.03 4
25 1.03 4
25 1.03 4
25 1.03 4
25 1.03 4
25 1.03 4

Pr/total)*100