Genome of Musa textilis TTT TAA TTA TAG AGC TAT GAC ACA ATC AAA CCC GAA

TGA ACA AAA TGT TGA ATT GAA TCG TAC CAG TCT ATA CTG GGG TTT ATT ACT AAT TTT TGT ACT TGC TGT TTT ATT TTC CAA TTA TTT CTT CAA TTG AAA GAA AGA AAG AGA ATA AAA ATT AAT ATC TCG ATA CTG TGT TAG TTT GGG CTT ACT TGT TTT ACA ACT TAA CTT AGC ATG GTC AGA TAT GAC CCC AAA TAA TGA TTA AAA ACA TGA ACA ACA AAA TAA AAG GTT AAT AAA GAA CTT AAC TTT CTT TCT TTC TCT TAT PR: AAG PF: TAA 5 TTT TGT ACT TGC TGT TTT ATT TTC CAA TTA TTT CTT CAA TTG AAA GAA AGA AGA ATA 3 5 AAA ATT AAT ATC TCG ATA CTG TGT TAG TTT GGG CTT ACT TGT TTT ACA ACT CTT AGC 3

Tm PR

= 4(G+C) + 2(A+T) = 4(8+6) + 2(20+26) = 4(14) + 2(46) = 56 + 92 = 148 Suhu Annealing = 148 5 = 143 Tm PF = 4(G+C) + 2(A+T) = 4(9+9) + 2(17+25) = 4(18) + 2(42) = 72 + 84 = 156 Suhu Annealing = 156 5 = 151 PCR Produk = 60