CRYOGENIC TRANSMISSION

ELECTRON MICROSCOPY (CRYO-TEM)

Information: size,shape,complex structures in
the solution state
Advantages:
Characterize in situ,in the solution state
Disadvatages:
Heavy technical skills for sample preparation

Zhong and Pochan, Polm.Rev,2010,50,287
CRYOGENIC TRANSMISSION
ELECTRON MICROSCOPY (CRYO-TEM)
Sample preparation
Mats Almgren *, Katarina Edwards, Go ran
Karlsson,Box 532, S-751 21 Uppsala, Sweden
Structures formed after addition of
CTAC(alkyltrimethylammonium chlo-ride) to sonicated and
centrifuged EPC(egg yolk lecithin) liposomes in 0.100 M NaCl
(a) The initial liposome preparation (b) 75% CTAC 24h (c) 80% CTAC 24h
(d) 60% CTAC 15 min (e) 60% CTAC 3h, (f) 60% CTAC 24h
Mats Almgren *, Katarina Edwards, Go ran
Karlsson,Box 532, S-751 21 Uppsala, Sweden
EPC: CHOLESTEROL=60:40 MOL%
EPC mixed with PEG-lipid (17.5%) without
cholesterol
Mats Almgren *, Katarina Edwards, Go ran
Karlsson,Box 532, S-751 21 Uppsala, Sweden
DSPC:CHO (60:40 molar ratio)

15 mol% PEG-lipid (MW 2000) added

Mats Almgren *, Katarina Edwards, Go ran
Karlsson,Box 532, S-751 21 Uppsala, Sweden
http://www.sciencedirect.com/science/article
/pii/S0378517312000178
http://www.sciencedirect.com/science/article
/pii/S092777651200714X
http://pubs.acs.org/doi/abs/10.1021/mp1002
96w
Xc tc phc rhodium c nh trn
cht mang polime ha tan
Tc gi: bergbreiter
Phn ng: hydro ha anken
hydro ha 1-dodecen v 1-octen (tan trong heptan)
Xc tc tan trong dung mi etanol/nc
Ti s dung: 4 ln

48
O CH
3
O
C H
3
NH
CH
3
NH
CH
3
CH
3
48
90% EtOH/ n
-
C
7
H
16
(1:1)
TEA, 70
o
C, 11h
49
Phn ring theo phng php pH
50

pH>7.5
pH<4.0
Phn ring theo phng php kt ta
51
DCC, DMAP, CH
2
Cl
2
C H
3
O
CH
3
C H
3
CH
2
Ru
3-
PCy
3
PCy
3
Cl
Cl
CPh
2
O
+
CH
3
C H
3
Ru
4-
Cl
Cl
PCy
3
CH
3
(OCH
2
CH
2
)
n+1
OCOCH
2
CH
2
CO
2
CH
3
(OCH
2
CH
2
)
n
OCH
2
CH
2
OCOCH
2
CH
2
CO
2
H
C H
2
N
CH
2
SO
2
C
6
H
4
CH
3
N
SO
2
C
6
H
4
CH
3
51
a)
b)
54
Cl
O
+
O H
2 Cl
O
+
Cl
OH
O H
54
Phn ring xc tc trong h hai pha
nc hu c
55
PPh
2
Ph
2
P
N H O
C H
3
CH
3
O
O
-
O
O
-
n
n
2
[Rh(NBD)]
+
CF
3
SO
3
H
Ph
CO
2
H
NHCOCH
3
HO
2
C
NHCOCH
3
H
H
Ph
55
H
2
(22 bar)
EtOAc/H
2
O
Xc tc nano trong ha hc xanh
Xc tc nano