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    This document outlines an experiment testing the effects of bignay extract on phenylhydrazine-induced anemic rats. The rats were divided into 5 groups, with groups 2-5 being induced with phenylhydrazine. Groups 3-5 were given different doses of bignay extract, group 2 was given a positive control vitamin B12, and group 1 was untreated. Blood samples were taken from all groups at various intervals to evaluate hematological effects, and subsets from groups 3-5 were sacrificed for histopathological and immunomodulatory testing of the liver and carbon clearance respectively.

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    This document outlines an experiment testing the effects of bignay extract on phenylhydrazine-induced anemic rats. The rats were divided into 5 groups, with groups 2-5 being induced with phenylhydrazine. Groups 3-5 were given different doses of bignay extract, group 2 was given a positive control vitamin B12, and group 1 was untreated. Blood samples were taken from all groups at various intervals to evaluate hematological effects, and subsets from groups 3-5 were sacrificed for histopathological and immunomodulatory testing of the liver and carbon clearance respectively.

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    © All Rights Reserved
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    3 views1 page

    Flowchart

    Uploaded by

    koikoroikoi
    This document outlines an experiment testing the effects of bignay extract on phenylhydrazine-induced anemic rats. The rats were divided into 5 groups, with groups 2-5 being induced with phenylhydrazine. Groups 3-5 were given different doses of bignay extract, group 2 was given a positive control vitamin B12, and group 1 was untreated. Blood samples were taken from all groups at various intervals to evaluate hematological effects, and subsets from groups 3-5 were sacrificed for histopathological and immunomodulatory testing of the liver and carbon clearance respectively.

    Copyright:

    © All Rights Reserved
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    of 1

    Biological

    Testing
    Acclimatization
    (1 week)
    Group 5
    (PHZ-induced)
    Bignay Extract
    500 mg/ kwb.w.
    Group 4
    (PHZ-induced)
    Bignay Extract
    500 mg/ kg b.w.
    Group 3
    (PHZ-induced)
    Bignay extract
    500 mg/ kg b.w.
    Group 2
    (PHZ-induced)
    Positive Control for
    Anti-anemic study
    Vitamin B
    12
    (1 mL)
    Experimental
    Groups
    Blood collection at 0 day, 4
    th
    day
    and 14
    th
    day for haematological
    evaluation
    Three (3) rats from groups 3, 4 & 5 (one from
    each group through random sampling) will be
    sacrificed for the histopathological evaluation
    of the liver on the 14
    th
    day
    Group 1
    Negative control

    Six (6) rats from groups 3, 4 & 5 (two from each
    group through random sampling) will be
    sacrificed for carbon clearance
    test(immunomodulatory study) on the 14
    th
    day

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