Bhagwan Mahavir College of M.Sc.

Biotechnology
INTERNAL EXAMINATION 2013-2014
Semester : 2
nd
sem. Time :
Date : /0 /2014 Total Marks:
Subject : MB 03 Microbial Growth and Nutrition

Q : 1 Answer the following in short. (05)
a) Define Nutrients.
b) Who developed pure culture technique?
c) Define pure culture.
d) Write the full form of MIP.
e) What do you mean by Colony?

Q : 2 Attempt the following: (Choose the best suitable option) (05)
a) Which is known as trace element for bacteria?
A. C B. H
C. Ni D. P


b) Which are the representative microorganisms of group
Photolithoautotrophs?

A. Purple sulfur Bacteria B. Green nonsulfur bacteria
C. Sulfur oxidizing bacteria D. Methanogens


c) Which molecule cannot be transferred by Passive Diffusion?
A. Water B. Carbon Dioxide
C. Glucose D. Oxygen


d) Protein molecule used in Chromosome movement is..
A. MreB B. MinCD
C. FtsZ D. MimiC


e) At which temperature Psychrophiles will grow?
A. 10-15C B. 25-40C
C. 37C D. 50-70C


Q : 4 Answer the following. (25)
a) Describe in detail: Chemical and Physical types of culture media.
b) Give a detailed account on Functional types of media.
c) Write a note on methods for isolation of pure cultures
d) Explain: Passive and facilitated diffusion.
e) Describe in detail about requirement of growth factors by bacteria.

BEST OF LUCK


Paper Setter: Dr. Naresh Butani