Thursday July 3

rd
, 2014 Lab reports are due 3502
-double spaced, regular sized margin
crude is the most amount of protein you can have, calculate the molecular weight of
the crude figure out the relative mobility of the bands and use the equation of the
line which gives you the log of the molecular weight
A-13 KDa
G-63 KDa
ONLY 2 decimal places
Find molecular weight of alpha lact likely 12 KDa

Lab reports:
longer is not better
the more boring it is and the more it is followed the layout the better
8 hours of effort


use headings
Order of writing:
1. Results
2. Map out discussion
3. Intro
4. Discussion
5. Conclusion
6. Abstract

No need for title page

Abstract:


Introduction: 4 PARAGRAPHS!!!!
-context and area of the work
background information
gives the reader information to appreciate the discussion point
-introduction to the topic area obtain a pure sample of alpha lact of bovine milk
using . .. . And SDS page was used to evaluate the efficacy of purification
What is milk? protein at the end of the composition
protein is subdivided into 2 categories: hydrophobicity and charge : casein and
whey
1-2 sentences of caseins: molecular weight
3 sentences of whey proteins :
table of whey proteins and caseins including examples, molecular weight pI

para 2
alpha lact is a globular protein with a pI of
percent, aromatic properties, disulfide bridge, alpha beta?

Lab 3 pI of alpha lact
Lab 4 shape of alpha lact


Para 3
Role of alpha lact and cancer treatment
Recently alpha lact has a role of anti-carcinogenic properties.
logically support the main idea,
get general and then more and more specific

Para 4:
-Statement of objectives and how you are going to meet them
The overall objective was to obtain a pure sample of alpha lact from bovine milk
Using .
Overall, purification was assessed using SDS page


METHOD
Refereeing to the lab manual
The experiment protocol to purify alpha lact was from the manual (reference)
State any changes from the protocol

RESULTs
- use subheadings, ie. Isoelectric acid purification
- 3 sentences, and then put them together
- first sentence: introduction of what you did and how you did it:
- second sentence: most specific result
- Third sentence: second most specific result

Ie.
Lab 3 the crude prep of alpha lactbumin was used to by applying


300 mM contained the highest fraction of protein 4.578mg/ml

overall, 347% was recovered and 345% was recovered in the 300mM fraction


Lab 2:
-table of observations

Lab 3:
-elution profile: NaCL vs. protein conc.
- purification table: NaCl, protein conc, total protein recovered, percent recovered

Lab 4:
-elution profile: NaCL vs. protein conc.
- purification table: NaCl, protein conc, total protein recovered, percent recovered


Lab 5:
-picture of the gel, containing the appropriate labels and titles
-table of calculated molecular weights

NO STANDARD CURVES
- dont include dilutions, dont care sample volumes, just analyzed statement

Lab 2:
-observation from the addition of Naso4 and acidification

Figures presented before the table

- have to make reference of the table and figure in the text

ie. 300mM eluted the highest concentration of protein 4.80832 (table 1)


DISCUSSION:
-paragraph for lab 2, para for 3, papar for 4, para for 5, and para for overall
- second sentence is a comparison to literature,
explain why is that way
bulk of the whey proteins have a very specific pI and will bind tightly to the column
relate the results to the protein you are studying
alpha lact as a pI of 2-9 (reference) and it was expected to be eluted from
if wash was really high why? Contained to contaminated caseins that were not
negatively charged
Lab 5 main specific results : crude protein fractions contained 16-28 kDa, how
does that compare, discuss the 50 kD in the crude IDENTIFY, which
purification step is the most effective statement and rational support

This protein is likely alpha-lact comparison to molecular weight,

CONCLUSION
restatement

REFERENCES
better to over-reference reference anything that is common knowledge
reference the first sentence and the last sentence dont reference every
sentence
reference pI values
6 references is more than enough