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  • Fgfrs Primers: Supplementary Table 1. Primer Sequences Used For Mutation Analysis of The FGFRS, Brachyury, Kras and Braf

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    This document lists the primer sequences and lengths that were used for mutation analysis of several genes, including the FGFR family of genes, Brachyury, KRAS, and BRAF. The primers were designed based on the 3' end of the transcribed sequence of each target gene using primer3 software to have lengths between 200-600 base pairs.

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    This document lists the primer sequences and lengths that were used for mutation analysis of several genes, including the FGFR family of genes, Brachyury, KRAS, and BRAF. The primers were designed based on the 3' end of the transcribed sequence of each target gene using primer3 software to have lengths between 200-600 base pairs.

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    Fgfrs Primers: Supplementary Table 1. Primer Sequences Used For Mutation Analysis of The FGFRS, Brachyury, Kras and Braf

    Uploaded by

    EstefaníaCarrillo
    This document lists the primer sequences and lengths that were used for mutation analysis of several genes, including the FGFR family of genes, Brachyury, KRAS, and BRAF. The primers were designed based on the 3' end of the transcribed sequence of each target gene using primer3 software to have lengths between 200-600 base pairs.

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    © All Rights Reserved
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    Supplementary Table 1.

    Primer sequences used for mutation analysis of the FGFRs, Brachyury, KRAS and BRAF
    FGFRs primers
    Gene (exon)
    FGFR1 (exon 3)

    Primer sequence
    F
    5- AGATGTGGAGCCTTGTCACC -3
    R
    5- CCTGTTGACCACATCACCTG -3
    F
    5- TGCCTGTCTCTCTTGGCTTT -3
    R
    5- CCTCCCCTGTTCCCATTACT -3
    F
    5- TGGGAAGCCCTGACTAAGAA -3
    R
    5- AAGCAAGGAATGCCTTCAAA -3
    F
    5- GTGGGGTTTCTTTGAGGTGA 3
    R
    5- CACCGGCTGGAAGACTAGGG -3
    F
    5- CTCCCCTGTGCTGCTTTC -3
    R
    5- CATCTGGAGCAGAGGGAATG -3
    F
    5- TTTACTCATGGAGGGGAAGC -3
    R
    5- CGAGACTCCATCGCAAAAA -3
    F
    5- GCGTTTTCCTTGCAGCGGCTGG - 3
    R
    5 - GTAAGTCACAGGATTCCCGTC - 3
    F
    5 - CTTTAGTAAGCCGCTGAAAGA -3
    R
    5- TTCACATGCCACAAAGGAA - 3
    F
    5- CGGGAAACACAAAAACATCA - 3
    R
    5- CTGAAGCCTCTCCACCTCTC -3
    F
    5- AGGCTTCCACTGCTGTGTCT -3
    R
    5- GCACTCGGCTCCTTTCTGTA - 3
    F
    5- AGGAAGTGCTGCCCAAATG - 3
    R
    5- TTAGTCCCTCAGCTGCCTGT - 3
    F
    5- GAAGGGGGTTGTTCAGAGG -3
    R
    5- CTGTGGGGGCAGATGACGCTCA -3
    F
    5- GCGTCGTGGAGAACAAGTTT -3
    R
    5- GAAGCTCCAACCCCTAGACC -3
    F
    5- GCCTATCGCTCTGCTCTCTC -3
    R
    5- GGCCGTAAGTCACAGGATTC -3
    F
    5- GCTCCTTCTCTCCAGGGTCT -3
    R
    5- TTCCACCAGCATTGAATGAA -3
    F
    5- TTCATTCAATGCTGGTGGAA -3
    R
    5- CCTCTGACTGGTGGCTGTTT -3
    F
    5- CTGCAGAGCTCAGGCTTCA -3
    R
    5- CGCCTTATTCGGGAACAG -3
    F
    5- CCCAGAGTGCTGAGGTGTG -3
    R
    5- ACCCAGGGTACCTCTGCAC -3
    F
    5- GCAGGGTAAGCAGGAGACAG -3
    R
    5- GACATGCTCTGGGGTCACA -3
    F
    5- GAGCTGGGAGGGACTGAGTT -3
    R
    5- ATGGAGAAAGTCCAGCCTCA -3
    F
    5- CTGTGGTGGGTCATGTCTGT -3
    R
    5- GGAGGAGGACGAGGAGTTCT -3

    Length bp
    226

    Gene (exon)

    Primer sequence

    Length bp

    KRAS (exon 2)

    F
    R
    F
    R
    F
    R
    F
    R

    270

    FGFR1 (exon 6
    FGFR1 (exon 11)
    FGFR1 (exon 12)
    FGFR1 (exon 14)
    FGFR2 (exon 5)
    FGFR2 (exon 7)
    FGFR2 (exon 11)
    FGFR2 (exon 12)
    FGFR3 (exon 2)
    FGFR3 (exon 3)
    FGFR3 (exons 4,5)
    FGFR3 (exon 6)
    FGFR3 (exon 7)
    FGFR3 (exon 8)
    FGFR3 (exon 9)
    FGFR3 (exon 15)
    FGFR3 (exon 17)
    FGFR4 (exon 7)
    FGFR4 (exon 9)
    FGFR4 (exon 16)

    314
    355
    302
    368
    314
    321
    302
    408
    389
    258
    538
    449
    275
    340
    430
    453
    476
    447
    601
    349

    Brachyury primers

    KRAS (exon 3)
    BRAF (exon 11)
    BRAF (exon 15)

    5GATACACGTCTGCAGTCAACTG--3
    5-GGTCCTGCACAGTAATATGC-3
    5-TGCACTGTAATAATCCAGACTGTG-3
    5-CTCCTTAATGTCAGCTTATTATATTCA-3
    5- TCCCTCTCAGGCATAAGGTAA -3
    5- CGAACAGTGAATATTTCCTTTGAT -3
    5- TCATAATGCTTGCTCTGATAGGA -3
    5- CTGATTTTTGTGAATACTGGGAAC TC-3

    240
    350
    220

    The primers were designed based on the 3 end transcribed sequence of the test gene with the primer3 design software (www.broad.mit.edu/cgibin/primer/primer3.cgi/primer3_www.cgi)

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