Physiological, nutritional and physical culture environment; by rooting treatments; through the induction

of culture photosynthesis; by the application of endophytic and epiphytic organisms. Thus, during growth
in vitro, plants can be prepared for optimal growth after their transfer to ex vitro conditions. This means
that the in vitro system may also be used to increase the quality of the plants. It should be noted that
because of the in vitro environment, the performance of the plant may suffer instead of benefit. Plant
growth regulators used during tissue culture may have unwanted after-effects