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  • Department of Biochemistry & Molecular Biology: Mid Semester Test 1 Tuesday 9 APRIL, 2013

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    William Thomas-Walters
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    This document provides instructions for a mid-semester exam on molecular cell biology. The exam contains two sections worth 15 marks each that must be completed within 15 minutes. Students are instructed to write their student number on each section and attempt all questions. The exam contributes 7.5% to the course grade. Section A contains two questions about membrane budding during vesicle transport and characteristics of protein translocation across the endoplasmic reticulum. Section B contains one question with three parts about NiNTA affinity chromatography used to isolate the TOM protein complex from Neurospora crassa and what electron microscopy revealed about this complex.

    Original Description:

    Past Exam Paper.

    Original Title

    bcmb30003 TEST1-Midsem2013

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    This document provides instructions for a mid-semester exam on molecular cell biology. The exam contains two sections worth 15 marks each that must be completed within 15 minutes. Students are instructed to write their student number on each section and attempt all questions. The exam contributes 7.5% to the course grade. Section A contains two questions about membrane budding during vesicle transport and characteristics of protein translocation across the endoplasmic reticulum. Section B contains one question with three parts about NiNTA affinity chromatography used to isolate the TOM protein complex from Neurospora crassa and what electron microscopy revealed about this complex.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    6 views5 pages

    Department of Biochemistry & Molecular Biology: Mid Semester Test 1 Tuesday 9 APRIL, 2013

    Uploaded by

    William Thomas-Walters
    This document provides instructions for a mid-semester exam on molecular cell biology. The exam contains two sections worth 15 marks each that must be completed within 15 minutes. Students are instructed to write their student number on each section and attempt all questions. The exam contributes 7.5% to the course grade. Section A contains two questions about membrane budding during vesicle transport and characteristics of protein translocation across the endoplasmic reticulum. Section B contains one question with three parts about NiNTA affinity chromatography used to isolate the TOM protein complex from Neurospora crassa and what electron microscopy revealed about this complex.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 5

    DEPARTMENTOFBIOCHEMISTRY&MOLECULARBIOLOGY

    BCMB30003MolecularAspectsofCellBiology

    MIDSEMESTERTEST1TUESDAY9THAPRIL,2013

    EXAMINATIONDURATION

    30MINUTES

    InstructionstoStudents:

    Thisexaminationpapercontains2sections.Eachsectionhasbeenallocated15marksforcompletion
    in15minoftheexaminationtime.

    WriteyourSTUDENTNUMBERwhereindicatedatthebeginningofEACHsection.

    Allquestionsinbothsectionsshouldbeattempted.

    Questionsmustbeansweredinthespacesprovided.

    Thispapercontributes7.5%ofthemarksforthesubjectBCMB30003

    ThisENTIREpapermustbereturnedattheendoftheexamination

    Page1of5

    SECTIONA(15marks)

    STUDENTNUMBER:

    Question1(7.5marks)

    Outlinethemolecularmechanismformembranebuddingandformationoftransportvesicles,givingCOPII
    derivedvesiclesastheexample.

    Page2of5

    Question2(7.5marks)

    A.

    Describethecharacteristicsoftheproteinsequencethatallowsnewlysynthesizedsolubleproteins
    tobetranslocatedacrossendoplasmicreticulummembranes(5marks)

    B.

    In12sentencesdescribeoneapproachtoidentifytheendoplasmicreticuluminculturedcelllinesby
    lightmicroscopy(2.5marks)

    Page3of5

    SECTIONB(15marks)

    STUDENTNUMBER:

    Question3(15marks)

    A.

    B.

    DescribethebasisofNiNTAaffinitychromatography.(5marks)

    HowwasthetechniqueofNiNTAaffinitychromatographyusedtoisolatetheTOMcomplexfrom
    Neurosporacrassa?(5marks)

    Page4of5

    C.

    WhatdidelectronmicroscopyoftheTOMcomplexpurifiedfromNeurosporacrassareveal?
    (5marks)

    ENDOFTEST
    Page5of5

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