RESKY DWIYANA PM

KELOMPOK III

REAKSI ASAM AMINO DAN PROTEIN

1. Tes Ninhidrin

HO

OH

+ R

C
C

CH

OH

COOH

+ R

C
C

NH2

CH + NH3 + CO2
O

hidrindantin

ninhidrin
O

O
OH

HO

+ NH3 +

C
OH

C
H

C
O

C
C

3 H2O

OH

diketohydrindylenediketohydrindamine

2. Reaksi Gugus rantai panjang (Gugus R )

O
HS - CH2 - CH - C - OH + Fe(CN)5 - NO - Na + NH4OH
NH2
O
NH4 - Fe(CN)5 - NO - S - CH2 - CH - C - OH + NaOH
NH2

3. Reaksi Biuret

a. Asam Aspartat
HOOC

CH2

CH

COOH

NaOH

+ CuSO4

NH2

b. Albumin
O
2NH2

CH

NH

CH

CH

NH

CH

NH

OH + 2NaOH

O
2NH2

CH

O
NH

CH

ONa + CuSO4

R
O

O
NH

CH

CH

NH

OH

Cu2+
O
OH

O
NH

CH

c. Glisin
H

CH

COOH

NaOH

CuSO4

NH2

d. Alanin
H3C

CH

CH
NH2

COOH

NaOH

CuSO4

NH

Na2SO4 + H2O

4. Reaksi Hopkins-Cole
a. Alanin
H3 C

CH

COOH

Pereaksi Hopkins

NH2

b. Glisin
H

CH

+ Pereaksi Hopkins

COOH

NH2

c. Asam Aspartat
COOH - CH2 CHNH2 COOH
d. Albumin
H

+ Pereaksi Hopkins
O

O
OH

HO

OH

HC

SO3H

SO3H

CHCOOH
H

OH

+
N

OH

H2SO4

NH2

CH2CHCOOH

- H2O

NH

N
HO

CH
C

+
N

CH3 CH

COOH

CH

O
COOH

OH

5. Rekasi Millon
a. Albumin
HO

CH2

CH

COOH + Hg(NO3)2

NH2

HOOC

CH2

CH2

Hg

CH2

CH
NH2

COOH + 2HNO3

b. Alanin

H3C

CH

COOH

Hg(NO3)2

NH2

c. Glisin
H

CH

COOH

Hg(NO3)2

NH2

d. Asam Aspartat
COOH - CH2 CHNH2 COOH

Hg(NO3)2