Professional Documents
Culture Documents
H.RAMLAN SADELI,dr.MS,SpMK
CLINICAL MYCOLOGY
Introduction to Mycology :
1. History, and development of mycology
2. Structure, morphology, classification and taxonomy of fungi
3. Growth and cultivation of fungi
4. Diagnostic and examination of fungal disease
Introduction to Mycology
In modern mycology, the fungi are placed within a separate Kingdom :
1. Monera
2. Protista
3. Fungi
4. Plantae
5. Animalia
Phyllum Eumycota, the four classes of fungi in which the human pathogens are
placed :
Fungi (Kingdom)
Eumycota (Phylum)
Classes : Zygomycetes
Ascomycetes - yeast
Basidiomycetes - mushrooms
Deuteromycetes - asexual (anamorph)
state only
Classes :
1. Zygomycetes :
is characterized by the production of large, non-septate (coenocytic) hyphae
and sporangia
are found everywhere, they produce diseases most often in diabetics
2. Ascomycetes :
are identified by their sexual (teleomorphic) state
since this state is not often seen in the laboratory, the fungi found as human
pathogens usually are not identified in this class
telemorph state is characterized by production of ascus (or sac) within which
sexual spore are produced (ascospores)
Classes :
3. Basidiomycetes :
are identified by their sexual (teleomorphic) state
the teleomorphic structure is the basidium and sexual spores, basidiospores,
are produced on the outside of the basidium
4. Deuteromycetes (fungi imperfecti) :
no sexual state is seen
are identified by the type of conidia and other asexual structures formed in
culture on standard media
Structures :
1. Yeast :
may bud from another mature cell
or from the septal area of certain hyphal fungi
2. Hyphae :
is the thread-like structure that form the cell body of most fungi
may be large ang without cross-walls (septae) :
non-septate or coenocytic; is found in the Zygomycetes
may be thinner and produced cross-walls (septae) :
septate hyphae; are formed by all other fungi
Structures :
3. Conidia : may be small (microconidia) or large (macroconidia)
blastoconidia : form as buds
arthroconidia : form directly from hyphal fragmentation
chlamydospore : form directly from the hyphae by a rounding up and
thickening of the wall
4. Sporangiospores :
asexual cells developed within a sporangium
produced by the Zygomycetes
5. Vesicle : is the swollen tip of a conidiophore or sporangiophore
6. Phialide : flask-shaped structure which produces phialoconidia
Collection of specimens :
1. Skin scrapings :
clean the lesion of dirt or any topical medicines
scrape the outer edge of the lesion with a scalple
collect the scrapings in a clean container
2. Hair : remove hair from the infected site with clean forceps
collect in a clean container
3. Biopsied tissue :
placed in a sterile containers;
add steril water or saline to keep the tissue moist
do not freeze the tissue
4. Exudate or pus :
should be aspirated from an unopened abscess
placed in a steril tube and taken directly to the laboratory
never let the specimen dry
Collection of specimens :
5. Sputum :
collect sputum early in the morning as soon as the patient awakens
before collecting the sputum, the patient should brush his teeth or remove his
dentures, then thoroughly rinse his mouth
ask the patient to take a deep cough and raise sputum from the lung
collect the sputum in a wide-mouthed, sterile container that can be tightly
closed to prevent leakage
send the specimen directly to the laboratory
Collection of specimens :
6. Blood : should be collected aseptically
placed in the culture medium at the patients bedside
7. Spinal fluid :
collect aseptically and place into a sterile tube
do not refrigerate spinal fluid
8. Urine : collect in asterile container
take directly to the laboratory
Processing of specimens :
Specimens should be processed as soon as possible :
to ensure that the infecting fungus does not die
to control contaminating organism
If the specimen cannot be promtly processed, it should be refrigerated (except
spinal fluid).
1. Sputum and bronchial washings :
examine specimen grossly for purulent or caseous material or particles
prepare smears and wet mounts and inoculate this material onto appropriate
culture media
Processing of specimens :
2. Spinal fluid, urine and pleural fluid :
concentrate the specimen by centrifugation
make a wet preparation of the sediment and inoculate the appropriate media
with the remaining sediment
3. Tissue taken by surgical procedure :
remove any caseous or purulent material and place onto the appropriate
media & prepare wet preparation & smears
cut the tissue into small pieces with sterile scissors and grind the tissue with
a sterile mortar and pestle
transfer the homogenized tissue to appropriate media
Processing of specimens :
Direct microscopic examination :
is an essential step in diagnosing a fungal disease
often provide a rapid, tentative diagnosis (without having to wait for the
culture to grow)
culture must always be made to correctly identify the fungus
most mycological specimens are examined in the fluid state (wet mount),
include a KOH (or NaOH) preparation, India ink. lactophenol-cotton blue
Processing of specimens :
Culture media for isolation and identification :
The proper selection of isolation media is critical to obtaining a laboratory
diagnosis of a fungal disease. If the wrong medium is used, the fungus causing
disease may not grow.
Culture media routinely used may be divided into two main
primary isolation media (non-selective or selective ---> may contain
antibiotics to inhibit rapidly growing fungi)
differential media; are used to identify selected genera or or species (by
stimulation of characteristic growth/sporulation; or by the production of
physiological reaction on these media)
Processing of specimens :
Culture media for isolation and identification :
the isolation medium selection depends upon :
the type of specimen (heavily contaminated, or sterile)
the suspected etiological agent
A non selective medium like Sabouraud dextrose agar (SDA) should be routinely
used because it will support the growth of almost all the medically important
fungi.
However, without the addition of selective agent (such as chloramphenicol and
cycloheximide) this medium is practically useless.
Processing of specimens :
Temparature of incubation :
is important in the primary isolation of fungi
may be room temparature (25 - 27C) but prefarably 30C
can act as a selective factor (incubation at 45C will inhibit most fungi and
bacteria, but not Aspergillus fumigatus)
Processing of specimes :
Primary isolation media :
non-selective : Sabouraud dextrose agar
Brain heart infusion agar
Blood agar base
selective : Sabouraud dextrose agar
Brain heart infusion agar
Blood base agar
)* penicillin, streptomycin or chloramphenicol
Differential media : Neger seed agar
Yeast assimilation media
Sporulation media : cornmeal agar
yeast extract agar
potato dextrose agar
Czapek agar
with antibiotics )*
or antibiotics and
cycloheximide
MYCOSES
Superficial mycoses
Cutaneous mycoses
Subcutaneous mycoses
Systemic mycoses : pathogenic
opportunistic
SUPERFICIAL MYCOSES
Disease
Agent
Pityriasis versicolor
Malassezia furfur
Pityriasis nigra
Exophiala hortae
Black piedra
Piedraia hortae
White piedra
Trichosporon beigelii
PITYRIASIS VERSICOLOR
the term versicolor is particularly appropriate, since color of the lesion varies
according to the normal skin pigmentation, exposure to sunlight & severity of
infection
lesion occur more often on the upper body, face, neck, arm
the reason for a change from normal flora status to a pathogenic agent are not
clear
PITYRIASIS VERSICOLOR
Laboratory diagnosis :
under uv ligth (Woods lamp) ---> Fluoresence : yellow
wet mount of skin scales : lesion contain short typical elements & spherical
cells (yeast) & this observation is virtually pathognomonic (spaghetti & meat
ball appearance)
culture identification is not diagnostic (may be positive from non-infective
person); but the organism can be cultured onto SDA (Sabouraud dextrose agar)
covered with olive oil
Treatment : selenium sulfide, sodium thiosulfate, miconazole; but recurrent is
frequent.
SUPERFICIAL MYCOSES
Treatment : keratolytic agent such as salicylic acid or sulfur have been used;
topical miconazol
SUPERFICIAL MYCOSIS
BLACK PIEDRA
infection of hair shaft (of the scalp), characterized by black, hard nodules on
the hair shaft; very difficult to remove the nodules
in some area of the world, the infection may be encouraged for cosmetic
purpose
diagnosed by direct examination of the hair & nodules; reveals hyphal strands
are often aligt along the periphery of the mature nodules; and the center of the
mass resembles organized tissue with area in which asci are produced
SUPERFICIAL MYCOSIS
WHITE PIEDRA
infection of hair shaft (moustache; axilla; pubis); characterized by soft, lightcolored nodules of the hair sharf, easily to remove
direct examination of the hair sharf reveal the mass of intertwined hyphae of
the nodules; often fragmented into arthroconidia
CUTANEUS MYCOSES
Dermatophytosis :
may involve the skin, hair, nails (parts of the body which contain keratin)
may be acquired from animal (zoophilic), soil (geophilic), in which lesion are
quite inflammatory & may heal spontaneously
may be acquired from human (anthropophilic); usually less inflamation but
may be chronic
dermatophytosis are classified by the area of the body involved
DERMATOPHYTES
Antrophophilic
Zoophilic
E.floccosum
M.canis
M.audouinii
M.nanum
T.rubrum
T.verrucosum
T.schonleini
T.equinum
T.tonsurans
M.gallinae
T.violaceum
T.mentagrophytes
T.frrugineum
var mentagrophytes
T.concentricum
T.mentagrophytes var interdigitale
Geophilic
M.gypseum
M.fulvum
T.ajelloi
T.terrestre
DERMATOPHYTOSIS
TINEA CAPITIS
Grapatches ringworm :
TINEA CAPITIS
Blackdot ringworm :
caused by T.tonsurans; occurs in adults & is a chronic infection
characterized by hair breakage, leaving follicles with dark conidia (the hair
shaft breakage right on the surface of the scalp);
may be results in alopecia; usually treated with griseofulvin or ketoconazol
Kerion :
occurs primarily in children; usually transmitted by pets; accordingly by farm
animals
is most commonly caused by M.canis or T.mentagrophytes; more
inflammatory & occurs with kerion
may results in inflammation, keloid, kerion, & alopecia
my heal spontaneously; but usually treated with antifungal
DERMATOPHYTOSIS
TINEA FAVOSA (FAVUS)
TINEA BARBAE :
is an acute or chronic folliculitis of the beard, neck or face
is most commonly cause by zoophilic dematophytes (T.verrucosum;
T.mentagrophytes)
results in pustular; or dry, scally lesion; my be superinfected with bacteria;
treated with griseofulvin
DERMATOPHYTOSIS
TINEA CORPORIS :
is fungal infection of the glabrous skin; most commonly caused by T.rubrum,
T.mentagrophytes & M.canis
is characterized by annular lesion with active border & may be vesicular or
pustular
is treated with topical antifungal (tolnaftate, myconazol) or griseofulvin
(systemic)
TINEA IMBRICATA
is caused by T.concentricum; occurs on Pacific Ocean Islands & numerous
countries of Asia
is characterized by concentric ring on the skin; may cover large area of the
body; the scally often overlap
is treated by griseofulvin
DERMATOPHYTOSIS
TINEA CRURIS = Jock itch
is an accute or chronic fungal infection of the groin
is caused by E.floccosum, T.rubrum, T.mentagrophytes,
has some predisposing factors such as : hyperhidrosis, obesity, diabetes,
pregnancy, fluor albus, neurodermatitis
is treated with tolnaftate, miconazol, ketoconazol
TINE APEDIS = Athlete foot
is an acute or chronic fungal infections of the feet; most commonly caused by
T.rubrum, T.mentagrophytes, E.floccosum
may be superinfected with bacteria, which may require antibiotic treatment
before tinea pedis is treated
DERMATOPHYTOSIS
TINEA PEDIS
Clinical features : Chronic intertriginous tine pedis :
results in white mascerated tissue bet ween the toes (the most common form)
is treated with tolnaftate or imidazole & by keeping the feet dry (by using
alumunium chroride) & aerated; if infections persist, griseofulvin or
ketoconazole is used.
Clinical features : chronic dry scally tinea pedis :
results in hyperkeratotic scales on the heel, sole, or side of the feet; also
known as mocasin foot
is treated with hyperkeratotic agent such as whitfield ointment & griseofulvin
DERMATOPHYTOSIS
TINEA PEDIS
Clinical features : vesicular tinea pedis
is characterized by vesicles & vesiculopustules
permanganate or Burrows solution is used to open vesicle;
dermatophytid reactions my occur;
griseofulvin is the treatment of choice
TINEA MANUM :
is chronic, unilateral fungal infection of the hand, caused by T.rubrum,
T.mentagrophytes, E.floccosum
is characterized by diffuse hyperkeratotic; exfoliative, vesicular;
treatment = tinea pedis
DERMATOPHYTOSIS
TINEA UNGUIUM :
course of disease
Tinea unguium
(Dermatophytes)
Onychomycosis
(Candida sp.)
distal proximal
proximal distal
debris
non
pain
non
non
thickness
DERMATOPHYTOSIS
Laboratory diagnosis :
DERMATOPHYTOSIS
Laboartory diagnosis :
species identification requires culture
culture identification is based primarily on the appearance of the asexual
reproductive conidia or the specific hyphae
while all of these species grow as molds, they have distinctive features
the reverse of colonies of some species may be pigmented (red, yellow) and
the tops may be fluffy, velvety; white or pigmented
this characteristics combine with the microscopic morphology generally
permit an identification
SUBCUTANEUS MYCOSES
Diseases
Agent
Mycetoma pedis
Eumycetoma Pseudoallescheria boydii; Madurella sp;
Acremonium sp; Fusarium sp;
Actinomycetoma
Actinomyces israeli; Nocardia sp;
sp; Acinomadura sp;
Chromoblastomycosis
Fonseca pedrosoi; Fonseca compecta;
Cladosporium carionii; Phialophora
verrucosa
Sporotrichosis Sporothrix schenckii
Rhinosporidiosis
Lobomycosis
Rhinosporidium seeberi
Loboa loboi
Streptomyces
SUBCUTANEUS MYCOSES
General characteristic :
SUBCUTANEUS MYCOSES
Actinomycetoma :
Pseudoallescheria boydii
Madurella sp;
Acremonium sp;
Fusarium sp;
.
Actinomyces israeli
Nocardia sp;
Streptomyces sp;
Actinomadura sp;
.
MYCETOMA PEDIS
Laboratory diagnosis :
direct microscopic examination : granules obtain from the draining tissues are
examined for ther gross physical characteristic
microscopic examination (wet mount with KOH) reveal that bacterial filament
less than 1 um; & fungi filamen (hyphae) greater than 1 um
Treatment :
eumycetoma : amphotericin B; azole compound; & nystatin topical;
actinomycetoma : sulfonamide
surgical excition or even amputation may indicated
SUBCUTANEUS MYCOSES
CHROMOBLASTOMYCOSIS :
CHROMOBLASTOMYCOSIS :
Laboratory diagnosis :
direct microscopic examination : wet mount --> examination of tissue from
lesion reveals characteristics pigmented (brown) sclerotic bodies a round, thickwalled form that have cross divisions
culture : is required to identified the etiologic agent specifically
the colonial morphology growth rate & microscopic morphology of conidial
productions (sporulations) are characteristic of the species
Treatment : surgical exicition; antifungal drugs
SUBCUTANEUS MYCOSES
SPOROTRICHOSIS
caused by Sporothrix schenckii, a dimorphic fungus, is found worldwide as
inhibitant in soil & decaying vegetation
is characterized by a lesion that begin as a movable nodule & subsequently
became necrotic
if it is untreated, new lesion appear along the lymphatic draining area (this
pattern is pathocnomonic for this form of sporotrichosing)
the infections is particularly associated with gardener --> rose gardener
syndrome
SPOROTRICHOSIS
in endemic area, a non-lymphatic or fixed form may be seen
single lesion, do not spead, often found on the face, neck or finger (occurs in
hypersensitive / allergic person)
another form of non-cutaneus, systemic infections is pulmonary infections; is
seldom diagnosed & often found in chronic alcoholic; infections is initiated
following inhalation of fungal conidia
SPOROTRICHOSIS
Laboartory diagnosis :
the appearance of the lymphatic form is very characteristic; however diagnosis
requeres isolation & culture identification diagnosis requires isolation & culture
identification
direct microscopic examination : material removed from such lesion can be
directly examined for cigar-shaped yeast cells
stain such as calcoflour white & fluorescence antibodies may be useful
however, the number of orgnaism is often too few for releable observation and
material should be submitted for fungal culture
SPOROTRICHOSIS
Laboratory diagnosis :
the organism is thermally dimorphic; in soil or at room temperature is grows
as mold with distinctive conidia that are produced in a pattern often described
as a daisy head
Treatment :
KI (pottasium iodida), oral; topical
amphotericin B
SUBCUTANEUS MYCOSES
RHINOSPORIDIOSIS
in tissue, the organism a large (6-300 um), spherical sporangia (sperula) that,
when mature, is filled with endospres; on lysis, these endospore then repeat
the development sequence
SUBCUTANEUS MYCOSES
Diseasea
Agent
Blastomycosis
Blastomycoss drmatitidis
Histoplasmosis
Histoplasma capsulatum
Coccidioidomycosis
Coccidioides immitis
Paracoccidioidomycosis
Paracoccidoides brasiliansis
BLASTOMYCOSIS
= Notrh American Blastomycosis
= Gilchists disease
Etiologic agent : Blastomyces dermatitidis, a dimorphic fungus that grows as
mold at room temperature and as a yeast at 35 - 370C
Epidemiology :
or decaying wood associated with soil, has been isolated several times, but
repeated isolation from the same sites were not succesful
most of the cases have been found in Noth America, but also prevalent in
Africa & has been reported in India, occurs most often in adult males
the lack of a specific skin test antigen has prevented the determination of the
prevalence of asymptomatic Blastomycosis in large population
BLASTOMYCOSIS
Cxlinical features :
the primary site of Blastomycisis is the lung, with mild infiltrat & few clinical
symptoms
in severe disease, pulmonary infiltrate may be more extensive & the patients
will have fever, cough & weight lose, nodular pulmonary lesion may occur
some cases may progres to chronic disease with pulmonary fibrosis & the
cavitation
the fungus may disseminate to any organ of the body, mostly skin & bone
skin lesion are frequently a manifestationof disseminated disease, with
dry & scaly, extensive granulomatous with vescle or pustule
BLASTOMYCOSISIS
Laboratory diagnosis :
Direct microscopic examination :
histiphatology : the yeast form is usually easily fount in infected tissue, are
best detected with PAS or GMS stain
BLASTOMYCISIS
Laboratory diagnosis :
Culture :
is the dimorphic fungus, that grws in the mycelial form (mold) at room
temperature & as a yeast at 370C
the mold form grows slowly, became visible in 7 - 10 days, the colony is
usually white & cottony
the yeast-like colony grows on blood agar at 370C after 3 - 4 days
Microscopic morpology :
the mold produces small, smooth walled cinidia & attached to the
conidiophores that arise directly from the hyphae
yaest are large, thick-walled, single-budding & the bud has a wide base (neck)
the diagnostic structure of B. dermatitidis
BLASTOMYCOSIS
Serology :
ID test is the most reliable, CFT not detect antibodies in all cases, will
cross-react with antibodies to H. capsulatum
HISTOPLASMOSIS
= reticuloendothelionsis
= Darlings disease
Etiologic agent : Histoplasma capsulatum, a dimorphic fungus, having a mold form at
room temperature & yeast form at 370C
Epidemiology :
H. capsulatum grows in soil, especially in soil that esriched with bat or bird manure
often be isolated from old building/caves, where birds/chickens or bats have roosted
H. capsulatum grows in soil in the mycelial form & large number of conidia are
produce
the disease is acquired by inhaling conidia & reported from most area of the world
HISTOPLASMOSIS
Clinical features :
is primarily a pulmonary disease; when conidia are inhaled, infections is established in
the lungs; the disease may be mild, with few or no symptom (95%)
the most severe form of histoplasmosis is disseminated disease; the fungus invade any
organs of body
HISTOPLASMOSIS
Laboratory diagnosis :
Direct microskopic examination : wet mount :
histopathology : the yeast form can be found in tissue removed from the
infected sites, ussually in the macrophage & in granulomas
HISTOPLASMOSIS
Culture :
colony morphology : H. capsulatum grows slowly in the mold form when incubated at
room temperature, appear in 7 - 10 days but conidia is not form until later; on SDA
( sabouraud dextrose agar ) the colony Is ussually white & cottony
microscopic morphology :
two types of conidia are prodeced by H. capsulatum small, pyriform smoth-walled
conidia (microconidia, 4 - 6m ) and large, round, thick-waled tuberculated conidia
( macroconidia, 8 - 18m )
the diagnostic conidia
to prove the identification of H. capsulatum, convert the mold form - yeast form; be
done by transferring the mold colony to blood agar & incubate at 37 0C in 3 - 5 days the
yeast colony will be white
brown
HISTOPLASMOSIS
Serology :
antibodies to the fungus are produced within 10 - 21 days after a person is infected by
H. capsulatum
CFT, measures both IgM & IgG, is quantitative test; ID test is a quantitative test
Treatment :
Amphotericin B, Ketoconazole
COCCIDIOIDOMYCOSIS
= valley fever
Etiologic agent : Coccidioides immitis, a biphasic fungal pathogen
Epidemiology :
C. immitis grows in semi-acrid, solid, is known to exist in North, Central,
& South American, especially California; its inhaled into the alveoli, where it
produces disease, either benign ( resembles flu ), or acute, depending on many
factors ( race; incculum )
Clinical features :
most is a benign disease, prodeces only mild symptoms; among certain races
( Filipinos, Black ), immunosupressed or the used of corticosteroids,
disseminated may occur
there is no site of predilection for this organism; any body tissue may become
infected
COCCIDIOIDOMYCOSIS
Laboratory diagnosis :
Direct microscopic examination :
COCCIDIOIDOMYCOSIS
Culture : Never work with culture on the laboratory bench OUTSIDE of a
biohazard hood !
Serology : used as diagnostic & prognostic tools; include CFT, latex aglutination,
ID test
Treatment : Amphotericin B, Ketoconazole
PARACOCCIDIOIDOMYCOSIS
= South American blastomycosis
Etiologic agent : Paracoccidioidomycosis brasiliaensis, a domorphic fungus that
grows as mold at room temperature & as a yeast at 370C / in infected tissues
Epidemiology :
the saprophytic habitat of P. brasiliensis is not known; endemic mostly in
South America
PARACOCCIDIOIDOMYCOSIS
Clinical features :
the primarily site of infection is the lung; disease may be benign, primary
pulmonary form or may disseminate to produce acute & chronic, progresive
disease, includes lymph nodes & skin
the primary benign form may ultimately results with some residual interstitial
fibrosis
progresive chronic pulmonary disease may involve all lobes of the lung;
produce extensive fibrosis
PARACOCCIDIOIDOMYCOSIS
Laboratory diagnosis :
Direct microscipic examination :
wet mount : appears a large, yeast-like cells ( 30 - 360 m ), budding with one or
more buds ( multiple buds ) with narrow necks
PARACOCCIDIOIDOMYCOSIS
Laboratory diagnosis :
Culture :
colony morphology : P. brasiliensis is a dimorphic fungus, grows slowly in the
mycelial form at room temperature; readily convert to the yeast phase when
grown at 370C on enriched media
PARACOCCIDIOIDOMYCOSIS
Laboratory diagnosis :
Serology : CFT & ID test have been shown to be reliable; however cross reactions
may occur
Tretment : Ketoconazole, Amphotericin B, Sulfadiazine
SYSTEMIC MYCOSES
Agents
Candidiasis
Cryptococcosis
Cryptococcus neoformans
Aspergillosis
Zygomycosis
SYSTEMIC MYCOSES
Pathogenic
Opportunistic
Agent
dimorphic fungus
non-dimorphic fungus
Port dentre
Disease
usually chronic
usually acute
Patients
SYSTEMIC MYCOSES
CANDIDIASIS = Candidosis
acute / chronic fungal infections, involving, the mouth, vagina, skin nails,
bronchi / lung, alimentary tract, urinary tract, blood steam and less commonly,
the heart or menungen
CANDIDIASIS
Candida albicans :
is part of the normal flora of the skin, mucous membranes & GI tract along
with other Candida sp.
form elongated budding forms called pseudohyphae, which are often seen in
clinical material along with true hyphae, blastoconidia & yaest cells
CANDIDIASIS
CANDIDIASIS
CANDIDIASIS
Clinical features : Cutaneus candidiasis :
is usually an extension of oral thrush & may include esophagitis & ultimately
the entire gastrintestinal tract
CANDIDIASIS
CANDIDIASIS
Clinical feature : Candidemia / blood borne infections :
CANDIDIASIS
Laboratory diagnosis :
C. albicans be identified by :
* germ tube test -- yeast germination in serum at 370C
* culture on corn-meal-agar -- reveals chlamydospres
* culture on Eosin-methylen-blue-agar : reveals spider colony
* fermentation test of : glucose, lactose, maltose, sacharose
CRIPTOCOCCOSIS
include subacute or chronic fungal infections involving the lungs, meninges, or less
commonly the skin, bones & other tissues
CRYPTOCOCCOSIS
= Busse-Buschkes disease
= European Blastomycosis
Clinical feature :
CRYPTOCOCCOSIS
Laboratory diagnosis :
ASPERGILLOSIS
is a ubiquitous filmentous fungus whose airborne spores are contantly in the air
is recognized both in tissue & in culture by its characteristic septate hyphae with
dichotomous branching, produced conidial heads with numerous conidia
ASPERGILLOSIS
ASPERGILLOSIS
it may begin as sinisitis or lungs; it disseminate to any part of the body, most
frequently brain
ASPERGILLOSIS
is an allergic disease, in which the organism colonies the mucous plugs form
in the lung, but does not invade lung tissue
caused by the genera Rhizopus, Mucor & Absidia; non-septate fungi; phylum
Zygomycota; grow repidly & predilection for invading blood vessels & the
brain
ZYGOMYCOSIS
Clinical features : Rhinocerebral infections
presents with facial swelling & blood tinged exudate in the turbinate bones &
eyes; lethargy & fixated pupil