Exerccio Tipagem

Objetivos:
- explorar a ferramenta PubMLST
- identificar as diferentes STs

Usando os dados dos 7 genes housekeeping abaixo, busque na ferramenta

PubMLST qual a ST correspondente de cada um e qual a origem da cepa
depositada no site.

Isolado 1
>acs
GGCCCGCTGGCCAACGGCGCCACCACCATTCTGTTCGAGGGCGTGCCGAACTACC
CCGAC
GTGACCCGCGTGGCGAAAATCATCGACAAGCACAAGGTCAACATCCTCTACACCGC
GCCG
ACCGCGATCCGCGCGATGATGGCCGAAGGCAAGGCGGCGGTGGCCGGTGCCGAC
GGTTCC
AGCCTGCGTCTGCTCGGTTCGGTGGGCGAGCCGATCAACCCGGAAGCCTGGCAGT
GGTAC
TACGAGACCGTCGGCCAGTCGCGCTGCCCGATCGTCGACACCTGGTGGCAGACCG
AGACC
GGCGCCTGCCTGATGACCCCGCTGCCGGGCGCCCACGCGATGAAGCCGGGCTCC
GCGGCC
AAGCCGTTCTTCGGCGTGGTCCCGGCGCTG

Isolado 2
>acs
GGCCCGTTGGCCAACGGCGCCACCACCATTCTGTTCGAGGGCGTGCCGAACTACC
CCGAC
GTGACCCGCGTGGCGAAAATCATCGACAAGCACAAGGTCAACATCCTCTACACCGC
GCCG
ACCGCGATCCGCGCGATGATGGCCGAAGGCAAGGCGGCGGTGGCCGGTGCCGAC
GGTTCC

AGCCTGCGTCTGCTCGGTTCGGTGGGCGAGCCGATCAACCCGGAAGCCTGGCAGT
GGTAC
TACGAGACCGTCGGCCAGTCGCGCTGCCCGATCGTCGACACCTGGTGGCAGACCG
AGACC
GGCGCCTGCCTGATGACCCCGTTGCCGGGCGCCCATGCGATGAAGCCGGGCTCC
GCGGCC
AAGCCGTTCTTCGGCGTGGTCCCGGCGCTG

Isolado 3
>acs
GGCCCGTTGGCCAATGGCGCTACCACCATTCTGTTCGAGGGCGTGCCGAACTACCC
CGAC
GTGACCCGCGTGGCGAAAATCATCGACAAGCACAAGGTCAACATCCTCTACACCGC
CCCG
ACCGCGATCCGCGCGATGATGGCCGAAGGCAAGGCGGCGGTGGCCGGTGCCGAC
GGTTCC
AGCCTGCGTCTGCTCGGTTCGGTGGGCGAGCCGATCAACCCGGAAGCCTGGCAGT
GGTAC
TACGAGACCGTCGGCCAGTCGCGCTGCCCGATCGTCGACACCTGGTGGCAGACCG
AGACC
GGCGCCTGCCTGATGACCCCGTTGCCGGGCGCCCATGCGATGAAGCCGGGCTCC
GCGGCC
AAGCCGTTCTTCGGCGTGGTCCCGGCGCTG

Isolado 4
>acs
GGCCCGCTGGCCAACGGCGCCACCACCATTCTGTTCGAGGGCGTGCCGAACTACC
CCGAC
GTGACCCGCGTGGCGAAAATCATCGACAAGCACAAGGTCAACATCCTCTACACCGC
GCCG
ACCGCGATCCGCGCGATGATGGCCGAAGGCAAGGCGGCGGTGGCCGGTGCCGAC
GGTTCC
AGCCTGCGTCTGCTCGGTTCGGTGGGCGAGCCGATCAACCCGGAAGCCTGGCAGT
GGTAC

TACGAGACCGTCGGCCAGTCGCGCTGCCCGATCGTCGACACCTGGTGGCAGACCG
AGACC
GGCGCCTGCCTGATGACCCCGCTGCCGGGCGCCCACGCGATGAAGCCGGGCTCT
GCAGCC
AAGCCGTTCTTCGGCGTGGTACCGGCACTG

Isolado 5
>acs
GGCCCGCTGGCCAACGGCGCCACCACCATTCTGTTCGAGGGCGTGCCGAACTACC
CCGAC
GTGACCCGCGTGGCGAAAATCATCGACAAGCACAAGGTCAACATCCTCTACACCGC
GCCG
ACCGCGATCCGCGCGATGATGGCCGAAGGCAAGGCGGCGGTGGCCGGTGCCGAC
GGTTCC
AGCCTGCGTCTGCTCGGTTCGGTGGGCGAGCCGATCAACCCGGAAGCCTGGCAGT
GGTAC
TACGAGACCGTCGGCCAGTCGCGCTGCCCGATCGTCGACACCTGGTGGCAGACCG
AGACC
GGCGCCTGCCTGATGACCCCGCTGCCGGGCGCCCACGCGATGAAGCCGGGCTCT
GCAGCC
AAGCCGTTCTTCGGCGTGGTACCGGCACTG

Isolado 1
>aro
ATGTCACCGTGCCGTTCAAGGAAGAGGCCTATCGTCTGGTGGACGAATTGAGCGAG
CGGG
CCACCCGGGCCGGGGCGGTGAACACCCTGATCCGCCTGGCCGACGGTCGCCTGC
GCGGCG

ACAACACCGACGGCGCGGGCTTGCTGCGGGACCTGACGGCGAACGCCGGGGTCG
AGCTGC
GCGGCAAGCGGGTTCTCCTGCTCGGCGCCGGCGGTGCGGTGCGCGGGGTGCTCG
AACCCT
TCCTCGGCGAGTGCCCGGCGGAGCTGCTGATCGCCAACCGCACGGCGCGGAAGG
CCGTGG
ATCTGGCCGAGCGGTTCGCCGATCTCGGCGCGGTGCGCGGCTGCGGTTTCGCCGA
GGTCG
AAGGTCCTTTCGACCTGGTCGTCAACGGCACCTCGGCCAGTCTTGCCGGCGACGTG
CCGC
CGCTGGCGCAGAGCGTGATCGAGCCCGGCCGTACCGTCTGCTACGACATGATGTAT
GCCA
AGGAACCGACCGCCTTCA

Isolado 2
>aro
ATGTCACCGTGCCGTTCAAGGAAGAGGCCTATCGTCTGGTGGACGAGTTGAGCGAG
CGGG
CCACCCGGGCCGGGGCGGTGAACACCCTGATCCGCCTCGCCGACGGTCGCCTGC
GCGGCG
ACAACACCGACGGCGCCGGCCTGCTGCGGGACCTGACGGCGAACGCCGGGGTC
GAGCTGC
GCGGCAAGCGGGTTCTCCTGCTCGGCGCCGGCGGTGCGGTGCGTGGGGTGCTCG
AACCCT
TCCTCGGCGAGTGCCCGGCGGAGGTGCTGATCGCCAACCGCACGGCGCGGAAGG
CCGTGG
ACCTGGCCGAGCGGTTCGCCGACCTCGGCGCGGTGCACGGCTGCGGTTTCGCCG
AGGTCG
AAGGGCCTTTCGACCTGATCGTCAACGGCACCTCGGCCAGTCTTGCCGGCGACGT
GCCGC
CGCTGGCGCAGAGCGTGATCGAGCCCGGCCGTACCGTTTGCTACGACATGATGTAT
GCCA
AGGAACCGACCGCCTTCA

Isolado 3
>aro
ATGTCACCGTGCCGTTCAAGGAAGAGGCCTATCGTCTGGTGGACGAATTGAGCGAG
CGGG
CCACCCGGGCCGGGGCGGTGAACACCCTGATCCGCCTGGCCGACGGTCGCCTGC
GCGGCG
ACAACACCGACGGCGCGGGCTTGCTGCGGGACCTGACGGCGAACGCCGGGGTCG
AGCTGC
GCGGCAAGCGGGTTCTCCTGCTCGGCGCCGGCGGTGCGGTGCGTGGGGTGCTCG
AACCCT
TCCTCGGCGAGTGCCCGGCGGAGTTGCTGATCGCCAACCGCACGGCGCGGAAGG
CCGTGG
ACCTGGCCGAGCGGTTCGCCGACCTCGGCGCGGTGCACGGCTGCGGTTTCGCCG
AGGTCG
AAGGGCCTTTCGACCTGATCGTCAACGGCACCTCGGCCAGTCTTGCCGGCGACGT
GCCGC
CGCTGGCGCAGAGCGTGATCGAGCCCGGCCGTACCGTCTGCTACGACATGATGTAT
GCCA
AGGAACCGACTGCCTTCA

Isolado 4
>aro
ATGTCACCGTGCCGTTCAAGGAAGAGGCCTATCGTCTGGTGGACGAATTGAGCGAG
CGGG
CCACCCGGGCCGGGGCGGTGAACACCCTGATCCGCCTCGCCGACGGTCGCCTGC
GCGGCG
ACAACACCGACGGCGCGGGCTTGCTGCGGGACCTGACGGCGAACGCCGGGGTCG
AGCTGC
GCGGCAAGCGGGTTCTCCTGCTCGGCGCCGGCGGTGCGGTGCGCGGGGTGCTCG
AACCCT
TCCTCGGCGAGTGCCCGGCGGAGTTGCTGATCGCCAACCGCACGGCGCGGAAGG
CCGTGG
ACCTGGCCGAGCGATTCGCCGATCTCGGCGCGGTGCGCGGCTGCGGTTTCGCCGA
GGTCG

AAGGGCCTTTCGACCTGATCGTCAACGGCACCTCGGCCAGTCTTGCCGGCGACGT
GCCGC
CGCTGGCGCAGAGCGGGATCGAGCCCGGCCGTACCGTCTGCTACGACATGATGTA
TGCCA
AGGAACCGACTGCCTTCA

Isolado 5
>aro
ATGTCACCGTGCCGTTCAAGGAAGAAGCCTATCGTCTGGTGGACGAGTTGAGCGAG
CGGG
CCACCCGGGCCGGGGCGGTGAACACCCTGATCCGCCTCGCCGACGGTCGCCTGC
GCGGCG
ACAACACCGACGGCGCCGGCCTGCTGCGGGACCTGACGGCGAACGCCGGGGTC
GAGCTGC
GCGGCAAGCGGGTTCTCCTGCTCGGCGCCGGCGGTGCGGTGCGTGGGGTGCTCG
AACCCT
TCCTCGGCGAGTGCCCGGCGGAGTTGCTGATCGCCAACCGCACGGCGCGGAAGG
CCGTGG
ACCTGGCCGAGCGGTTCGCCGACCTCGGCGCGGTGCACGGCTGCGGTTTCGCCG
AGGTCG
AAGGGCCTTTCGACCTGATCGTCAACGGCACCTCGGCCAGTCTTGCCGGCGACGT
GCCGC
CGCTGGCGCAGAGCGTGATCGAGCCCGGCCGTACCGTCTGCTACGACATGATGTAT
GCCA
AGGAACCGACTGCCTTCA

Isolado 1
>gua
CTGCTAGGCCTCTCCGGCGGCGTGGACTCCTCGGTGGTCGCCGCGCTGTTGCACA
AGGCC
ATCGGCGACCAACTGACCTGCGTGTTCGTCGACAACGGCCTGCTGCGCCTGCACG
AAGGC

GACCAGGTGATGGCCATGTTCGCCGAGAACATGGGCGTGAAGGTGATCCGCGCCA
ACGCC
GAGGACAAGTTCCTCGGCCGCCTGGCCGGCGTCGCCGACCCGGAAGAGAAGCGC
AAGATC
ATCGGCCGCACCTTCATCGAAGTCTTCGACGAAGAAGCCACCAAGCTGCAGGACGT
GAAG
TTCCTCGCCCAGGGCACCATCTACCCCGACGTGATCGAGTCGGCCGGCGCCAAGA
CCGGC
AAGGCCCACGTGA

Isolado2
>gua
CTGCTAGGCCTCTCCGGCGGCGTGGACTCCTCGGTGGTCGCCGCGCTGTTGCACA
AGGCC
ATCGGCGACCAACTGACCTGCGTGTTCGTCGACAACGGCCTGCTGCGCCTGCACG
AAGGC
GACCAGGTGATGGCCATGTTCGCCGAGAACATGGGCGTGAAGGTGATCCGCGCCA
ACGCC
GAGGACAAGTTCCTCGGCCGCCTGGCCGGCGTCGCCGACCCGGAAGAGAAGCGC
AAGATC
ATCGGCCGCACCTTCATCGAAGTCTTCGACGAAGAAGCCACCAAGCTGCAGGACGT
GAAG
TTCCTCGCCCAGGGCACCATCTACCCCGACGTGATCGAGTCGGCCGGCGCCAAGA
CCGGC
AAGGCCCACGTGA

Isolado 3
>gua
CTGCTCGGCCTCTCCGGTGGCGTGGACTCCTCGGTGGTCGCCGCGCTGCTGCACA
AGGCC
ATCGGCGACCAACTGACCTGCGTGTTCGTCGACAACGGCCTGCTGCGCCTGCACG
AAGGC
GACCAGGTGATGGCCATGTTCGCCGAGAATATGGGCGTGAAGGTGATCCGCGCCA
ACGCC

GAGGACAAGTTCCTCGGTCGCCTGGCCGGCGTCGCCGACCCGGAAGAGAAGCGC
AAGATC
ATCGGCCGCACCTTCATCGAAGTCTTCGACGAAGAAGCCACCAAGCTGCAGGACGT
GAAG
TTCCTCGCCCAGGGCACCATCTACCCCGACGTGATCGAGTCGGCCGGCGCCAAGA
CCGGC
AAGGCCCATGTGA

Isolado 4
>gua
CTGCTAGGCCTCTCCGGCGGCGTGGACTCCTCGGTGGTCGCCGCGCTGTTGCACA
AGGCC
ATCGGCGACCAACTGACCTGCGTGTTCGTCGACAACGGCCTGCTGCGCCTGCACG
AAGGC
GACCAGGTGATGGCCATGTTCGCCGAGAACATGGGCGTGAAGGTGATCCGCGCCA
ACGCC
GAGGACAAGTTCCTCGGCCGCCTGGCCGGCGTCGCCGACCCGGAAGAGAAGCGC
AAGATC
ATCGGCCGCACCTTCATCGAAGTCTTCGACGAAGAAGCCACCAAGCTGCAGGACGT
GAAG
TTCCTCGCCCAGGGCACCATCTACCCCGACGTGATCGAGTCGGCCGGCGCCAAGA
CCGGC
AAGGCCCACGTGA

Isolado 5
>gua
CTGCTCGGCCTCTCCGGCGGCGTGGACTCCTCGGTGGTCGCCGCGCTGTTGCACA
AGGCC
ATCGGCGACCAACTGACCTGCGTGTTCGTCGACAACGGCCTGCTGCGCCTGCACG
AAGGC
GACCAGGTGATGGCCATGTTCGCCGAGAACATGGGCGTGAAGGTGATCCGCGCCA
ACGCC
GAGGACAAGTTCCTCGGCCGCCTGGCCGGCGTCGCCGACCCGGAAGAGAAGCGC
AAGATC

ATCGGCCGCACCTTCATCGAAGTCTTCGACGAAGAAGCCACCAAGCTGCAGGACGT
GAAG
TTCCTCGCCCAGGGCACCATCTACCCCGACGTGATCGAGTCGGCCGGCGCCAAGA
CCGGC
AAGGCCCACGTGA

Isolado 1
>mut
CTGCAGGAAGTCATCAAGCGCCTGGCGCTGGCCCGTTTCGACGTGGCTTTCCACCT
GCGC
CACAACGGCAAGACCATCTTCGCCCTGCACGAGGCGCGAGACGAGCTGGCCCGT
GCGCGC
CGGGTCGGCGCGGTGTGCGGCCAGGCATTCCTCGAGCAGGCGCTGCCGATCGAG
GTCGAG
CGCAACGGCCTGCACCTGTGGGGCTGGGTCGGCTTGCCGACCTTCTCCCGCAGCC
AGCCG
GACCTGCAGTACTTCTATGTGAACGGGCGCATGGTGCGCGACAAGCTGGTCGCCC
ACGCG
GTGCGCCAGGCTTATCGCGACGTGCTGTACAACGGCCGGCATCCGACCTTCGTGCT
GTTC
TTCGAAGTCGATCCGGCGGTGGTGGACGTCAACGTGCACCCGACCAAGCACGAAG
TTCGC
TTCCGTGACAGCCGGATGGTCC

Isolado 2
>mut
CTGCAGGAGGTCATCAAGCGCCTGGCGCTGGCCCGCTTCGACGTGGCTTTCCACC
TGCGC
CACAACGGCAAGACCATCTTCGCCCTGCACGAGGCGCGAGACGAGCTGGCCCGC
GCGCGC

CGGGTCGGCGCGGTGTGCGGCCAGGCATTCCTCGAGCAGGCGCTGCCGATCGAG
GTCGAG
CGCAACGGCCTGCACCTGTGGGGCTGGGTCGGCTTGCCGACCTTCTCCCGCAGCC
AGCCG
GACCTGCAGTACTTCTATGTGAACGGGCGCATGGTGCGCGACAAGCTGGTCGCCC
ACGCG
GTGCGCCAGGCTTATCGCGACGTGCTGTACAACGGCCGGCATCCGACCTTCGTGCT
GTTC
TTCGAAGTCGATCCGGCGGTGGTGGACGTCAACGTGCACCCGACCAAGCACGAAG
TTCGC
TTCCGTGACAGCCGGATGGTCC

Isolado 3
>mut
CTGCAGGAAGTCATCAAGCGCCTGGCGCTGGCCCGTTTCGACGTGGCTTTCCACCT
GCGC
CACAACGGCAAGACCATCTTCGCCCTGCACGAGGCGCGAGACGAGCTGGCCCGC
GCGCGC
CGGGTCGGCGCGGTGTGCGGCCAGGCATTCCTCGAGCAGGCGCTGCCGATCGAG
GTCGAG
CGCAACGGTCTGCACCTGTGGGGCTGGGTCGGCTTGCCGACCTTCTCCCGCAGCC
AGCCG
GACCTGCAGTACTTCTATGTGAACGGGCGCATGGTGCGCGACAAGCTGGTCGCCC
ACGCG
GTGCGCCAGGCTTATCGCGACGTGCTGTACAACGGCCGGCATCCGACCTTCGTGCT
GTTC
TTCGAAGTCGATCCGGCGGTGGTGGACGTCAACGTGCACCCGACCAAGCACGAAG
TTCGC
TTCCGTGACAGCCGGATGGTCC

Isolado 4
>mut
CTGCAGGAGGTCATCAAGCGCCTGGCGCTGGCCCGCTTCGACGTGGCTTTCCACC
TGCGC

CACAACGGCAAGACCATCTTCGCCCTGCACGAGGCGCGAGACGAGCTGGCCCGC
GCGCGC
CGGGTCGGCGCGGTGTGCGGCCAGGCATTCCTCGAGCAGGCGCTGCCGATCGAG
GTCGAG
CGCAACGGCCTGCACCTGTGGGGCTGGGTCGGCTTGCCGACCTTCTCCCGCAGCC
AGCCG
GACCTGCAGTACTTCTATGTGAACGGGCGCATGGTGCGCGACAAGCTGGTCGCCC
ACGCG
GTGCGCCAGGCTTATCGCGACGTGCTGTACAACGGCCGGCATCCGACCTTCGTGCT
GTTC
TTCGAAGTCGATCCGGCGGTGGTGGACGTCAACGTGCACCCGACCAAGCACGAAG
TTCGC
TTCCGTGACAGCCGGATGGTCC

Isolado 5
>mut
CTGCAGGAAGTCATCAAGCGCCTGGCGCTGGCCCGTTTCGACGTGGCTTTCCACCT
GCGC
CACAACGGCAAGACCATCTTCGCCCTGCACGAGGCGCGAGACGAGCTGGCCCGC
GCGCGC
CGGGTCGGCGCGGTGTGCGGCCAGGCATTCCTCGAGCAGGCGCTGCCGATCGAG
GTCGAG
CGCAACGGCCTGCACCTGTGGGGTTGGGTCGGCTTGCCGACCTTCTCCCGCAGCC
AGCCG
GACCTGCAGTACTTCTATGTGAACGGGCGCATGGTGCGCGACAAGCTGGTCGCCC
ACGCG
GTGCGCCAGGCTTATCGCGACGTGCTGTACAACGGCCGGCATCCGACCTTCGTGCT
GTTC
TTCGAAGTCGATCCGGCGGTGGTGGACGTCAACGTGCACCCGACCAAGCACGAAG
TTCGC
TTCCGTGACAGCCGGATGGTCC

Isolado1

>nuo
ATGTTCCTCAACCTCGGCCCGAACCACCCGTCCGCCCACGGCGCGTTCCGCATCAT
CCTG
CAACTGGACGGCGAGGAGATCATCGACTGCGTCCCGGAGATCGGCTACCACCACC
GCGGC
GCCGAGAAGATGGCCGAGCGCCAGTCCTGGCACAGTTTCATCCCCTACACCGACC
GCATC
GACTACCTCGGCGGGGTGATGAACAACCTGCCCTACGTACTCTCGGTGGAGAAGCT
CGCC
GGGATCAAGGTGCCGCAGCGGGTCGACGTGATCCGGATCATGATGGCGGAGTTCTT
CCGT
ATCCTGAACCACCTGCTGTACCTGGGCACCTATATCCAGGACGTCGGCGCCATGAC
CCCG
GTGTTC

Isolado 2
>nuo
ATGTTCCTCAACCTCGGCCCGAACCACCCGTCCGCCCACGGCGCGTTCCGCATCAT
CCTG
CAACTGGACGGCGAGGAGATCATCGACTGCGTCCCGGAGATCGGCTACCACCACC
GCGGC
GCCGAGAAGATGGCCGAGCGCCAGTCCTGGCACAGTTTCATCCCCTACACCGACC
GCATC
GACTACCTCGGCGGGGTGATGAACAACCTGCCCTACGTACTCTCGGTGGAGAAGCT
CGCC
GGGATCAAGGTGCCCCAGCGGGTCGACGTGATCCGGATCATGATGGCGGAGTTCTT
CCGT
ATCCTGAAC

Isolado 3
>nuo

ATGTTCCTCAACCTCGGCCCGAACCACCCGTCCGCCCACGGCGCGTTCCGCATCAT
CCTG
CAACTGGACGGCGAGGAGATCATCGACTGCGTCCCGGAGATCGGCTACCACCACC
GCGGC
GCCGAGAAGATGGCCGAGCGCCAGTCCTGGCACAGTTTCATCCCTTACACCGACCG
CATC
GACTACCTCGGCGGGGTGATGAACAACCTGCCCTACGTACTCTCGGTGGAGAAGCT
CGCC
GGGATCAAGGTGCCGCAGCGGGTCGACGTGATCCGGATCATGATGGCGGAGTTCTT
CCGT
ATCCTGAACCACCTGCTGTACCTGGGCACCTATATCCAGGACGTCGGCGCAATGAC
CCCG
GTGTTC

Isolado 4
>nuo
ATGTTCCTCAACCTCGGCCCGAACCACCCGTCCGCCCACGGCGCGTTCCGCATCAT
CCTG
CAACTGGACGGCGAGGAGATCATCGACTGCGTCCCGGAGATCGGCTACCACCACC
GCGGC
GCCGAGAAGATGGCCGAGCGCCAGTCCTGGCACAGTTTCATCCCCTACACCGACC
GCATC
GACTACCTCGGTGGGGTGATGAACAACCTGCCCTACGTACTCTCGGTGGAGAAGCT
CGCC
GGGATCAAGGTGCCGCAGCGGGTCGACGTGATCCGGATCATGATGGCGGAGTTCTT
CCGT
ATCCTGAACCACCTGCTGTACCTGGGCACCTATATCCAGGACGTCGGCGCCATGAC
CCCG
GTGTTC

Isolado 5
>nuo
ATGTTCCTCAACCTCGGCCCGAACCACCCGTCCGCCCACGGCGCGTTCCGCATCAT
CCTG

CAACTGGACGGCGAGGAGATCATCGACTGCGTCCCGGAGATCGGCTACCACCACC
GCGGC
GCCGAGAAGATGGCCGAGCGCCAGTCCTGGCACAGTTTCATCCCCTACACCGACC
GCATC
GACTACCTCGGCGGGGTGATGAACAACCTGCCCTACGTACTCTCGGTGGAGAAGCT
CGCC
GGGATCAAGGTGCCGCAGCGGGTCGACGTGATCCGGATCATGATGGCGGAGTTCTT
CCGT
ATCCTGAACCACCTGCTGTACCTGGGCACCTATATCCAGGACGTCGGCGCCATGAC
CCCA
GTGTTC

Isolado1
>pps
CATCGTCCAGGCACGCCCGGAAACCGTGAAGAGCCGCGCCAGCGCCACGGTCATG
GAGCG
CTACCTGCTGAAAGAGAAGGGGACCGTCCTGGTGGAAGGACGTGCCATCGGCCAG
CGCAT
CGGTGCCGGTCCGGTCAAGGTGATCAACGACGTGTCGGAAATGGACAAGGTCCAA
CCGGG
TGACGTCCTGGTCTCCGACATGACCGACCCGGACTGGGAGCCGGTGATGAAGCGC
GCCAG
CGCCATCGTCACCAACCGCGGCGGGCGCACCTGCCACGCGGCGATCATCGCTCGC
GAACT
GGGCATCCCGGCGGTGGTCGGTTGCGGCAATGCCACCCAGATCCTGCAGGATGGC
CAGGG
GGTGACCGTT

Isolado 2
>pps
CATCGTCCAGGCACGCCCGGAAACCGTGAAGAGCCGCGCCAGCGCCACGGTCATG
GAGCG

CTACCTGCTGAAAGAGAAGGGGACCGTCCTGGTGGAAGGGCGTGCCATCGGCCA
GCGCAT
CGGTGCCGGTCCGGTCAAGGTGATCAACGACGTGTCGGAAATGGACAAGGTCCAA
CCGGG
TGACGTCCTGGTCTCCGACATGACCGACCCGGACTGGGAGCCGGTGATGAAGCGC
GCCAG
CGCCATCGTCACCAACCGCGGCGGGCGTACCTGCCACGCGGCGATCATCGCTCGC
GAACT
GGGCATCCCGGCGGTGGTCGGTTGCGGCAACGCCACCCAGATCCTGCAGGATGGC
CAGGG
GGTGACCGTT

Isolado 3
>pps
CATCGTCCAGGCACGCCCGGAAACCGTGAAGAGCCGCGCCAGCGCCACGGTCATG
GAGCG
CTACCTGCTGAAAGAGAAGGGGACCGTCCTGGTGGAAGGACGTGCCATCGGCCAG
CGCAT
CGGTGCCGGTCCGGTCAAGGTGATCAACGACGTGTCGGAAATGGACAAGGTCCAA
CCGGG
TGACGTCCTGGTCTCCGACATGACCGACCCGGACTGGGAGCCGGTGATGAAGCGC
GCCAG
CGCCATCGTCACCAACCGCGGCGGGCGTACCTGCCACGCGGCGATCATCGCTCGC
GAACT
GGGCATCCCGGCGGTGGTCGGTTGCGGCAACGCCACCCAGATCCTGCAGGATGG
GCAGGG
GGTGACCGTT

Isolado 4
>pps
CATCGTCCAGGCACGCCCGGAAACCGTGAAGAGCCGCGCCAGCGCCACGGTCATG
GAGCG
CTACCTGCTGAAAGAGAAGGGGACCGTCCTGGTGGAAGGACGTGCCATTGGCCAG
CGCAT

CGGTGCCGGTCCGGTCAAGGTGATCAACGACGTGTCGGAAATGGACAAGGTCCAA
CCGGG
TGACGTCCTGGTCTCCGACATGACCGACCCGGACTGGGAGCCGGTGATGAAGCGC
GCCAG
CGCCATCGTCACCAACCGCGGCGGGCGCACCTGCCACGCGGCGATCATCGCTCGC
GAACT
GGGCATCCCGGCGGTGGTCGGTTGCGGCAATGCCACCCAGATCCTGCAGGATGGC
CAGGG
GGTGACCGTT

Isolado 5
>pps
CGTCGTCCAGGCGCGCCCGGAAACCGTGAAGAGCCGCGCCAGCGCCACGGTCAT
GGAGCG
CTACCTGCTGAAAGAGAAGGGGACCGTCCTGGTGGAAGGGCGTGCCATCGGCCA
GCGCAT
CGGTGCCGGTCCGGTCAAGGTGATCAACGACGTGTCGGAAATGGACAAGGTCCAA
CCGGG
TGACGTCCTGGTCTCCGACATGACCGACCCGGACTGGGAGCCAGTGATGAAGCGC
GCCAG
CGCCATCGTCACCAACCGCGGCGGGCGCACCTGCCACGCGGCGATCATCGCTCGC
GAACT
GGGCATCCCGGCGGTGGTCGGTTGCGGCAACGCCACCCAGATCCTGCAGGATGGC
CAGGG
GGTGACCGTT

Isolado 1
>trp
TGTCGTGGGCAGCTCGCCGGAGGTGCTGGTACGGGTCGAGGATGGCCTGGTGAC
GGTGCG
CCCGATCGCCGGTACCCGTCCGCGCGGGATCAACGAAGAGGCCGACCTGGCGCT
GGAGCA
GGATCTGCTGTCGGACGCCAAGGAGATCGCCGAGCACCTGATGCTGATCGACCTG
GGGCG

CAACGACGTGGGGCGGGTGTCCGATATCGGCGCGGTGAAGGTCACCGAAAAAATG
GTGAT
CGAACGTTACTCCAACGTCATGCACATCGTGTCCAACGTCACCGGGCAATTGCGCG
AGGG
GCTCAGCGCGATGGACGCGCTGCGGGCGATTCTGCCGGCGGGCACTCTATCCGGC
GCGCC
GAAGATCCGCGCCATGGAGATCATCGACGAGCTGGAGCCGGTCAAGCGTGGAGTC
TACGG
CGGCGCGGTCGGCTACCTGGCAT

Isolado 2
>trp
TGTCGTGGGCAGCTCGCCGGAGGTGCTGGTACGGGTCGAGGACGGCCTGGTGAC
GGTGCG
CCCGATCGCCGGTACCCGTCCGCGCGGGATCAACGAAGAGGCCGACCTGGCACTG
GAGCA
GGATCTGCTGTCGGACGCCAAGGAGATCGCCGAGCACCTGATGCTGATCGACCTG
GGGCG
CAACGACGTGGGGCGGGTGTCCGATATCGGCGCGGTGAAGGTCACCGAAAAAATG
GTGAT
CGAACGTTACTCCAACGTCATGCACATCGTGTCCAACGTCACCGGGCAATTGCGCG
AGGG
GCTCAGCGCGATGGACGCGCTGCGGGCGATTCTGCCGGCGGGCACTCTATCCGGC
GCGCC
GAAGATCCGCGCCATGGAGATCATCGACGAGCTGGAGCCGGTCAAGCGTGGAGTC
TACGG
CGGCGCGGTCGGCTACCTGGCAT

Isolado 3
>trp
TGTCGTGGGTAGCTCGCCGGAGGTGCTGGTACGGGTCGAGGATGGCCTGGTCACG
GTGCG
CCCGATCGCCGGCACCCGTCCGCGCGGGATCAACGAAGAGGCCGACCTGGCGCT
GGAGCA

GGATCTGCTATCGGACGCCAAGGAGATCGCCGAGCACCTGATGCTGATCGACCTGG
GGCG
CAACGACGTGGGTCGAGTGTCCGACATCGGCGCGGTGAAGGTCACCGAAAAAATG
GTGAT
CGAACGTTACTCCAACGTCATGCACATCGTGTCCAACGTCACCGGGCAATTGCGCG
AGGG
GCTCAGCGCGATGGACGCGCTGCGGGCGATCCTGCCGGCGGGTACGCTGTCCGG
CGCGCC
GAAGATCCGCGCCATGGAGATCATCGACGAGCTGGAGCCGGTCAAGCGTGGAGTC
TACGG
CGGCGCGGTCGGCTACCTGGCAT

Isolado 4
>trp
TGTCGTGGGCAGCTCGCCGGAGGTGCTGGTACGGGTCGAGGATGGCCTGGTGAC
GGTGCG
CCCGATCGCCGGTACCCGTCCGCGCGGGATCAACGAAGAGGCCGACCTGGCGCT
GGAGCA
GGATCTGCTGTCGGACGCCAAGGAGATCGCCGAGCACCTGATGCTGATCGACCTG
GGGCG
CAACGACGTGGGGCGGGTGTCCGATATCGGCGCGGTGAAGGTCACCGAAAAAATG
GTGAT
CGAACGTTACTCCAACGTCATGCACATCGTGTCCAACGTCACCGGGCAATTGCGCG
AGGG
GCTCAGCGCGATGGACGCGCTGCGGGCGATCCTGCCGGCGGGCACTCTATCCGGC
GCGCC
GAAGATCCGCGCCATGGAGATCATCGACGAGCTGGAGCCGGTCAAGCGTGGAGTC
TACGG
CGGTGCGGTCGGCTACCTGGCAT

Isolado 5
>trp
TGTCGTGGGCAGCTCGCCGGAGGTGCTGGTACGGGTCGAGGATGGCCTGGTGAC
GGTGCG

CCCGATCGCCGGTACCCGTCCGCGCGGGATCAACGAAGAGGCCGACCTGGCACTG
GAGCA
GGATCTGCTGTCGGACGCCAAGGAGATCGCCGAGCACCTGATGCTGATCGACCTG
GGGCG
CAACGACGTGGGGCGGGTGTCCGATATCGGCGCGGTGAAGGTCACCGAAAAAATG
GTGAT
CGAACGTTACTCCAACGTCATGCACATCGTGTCCAACGTCACCGGGCAATTGCGCG
AGGG
GCTCAGCGCGATGGACGCGCTGCGGGCGATTCTGCCGGCGGGCACTCTATCCGGC
GCGCC
GAAGATCCGCGCCATGGAGATCATCGACGAGCTGGAGCCGGTCAAGCGTGGAGTC
TACGG
CGGCGCGGTCGGCTACCTGGCAT