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  • Lamda Bokum

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    This document contains data from restriction enzyme digests of lambda DNA. It lists the distance migrated and estimated base pairs for uncut lambda DNA and digests with PstI, EcoRI, and HindIII. It also shows a linear regression analysis with distance vs log10 base pairs with R2 = 0.9993, indicating the relationship is linear.

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    This document contains data from restriction enzyme digests of lambda DNA. It lists the distance migrated and estimated base pairs for uncut lambda DNA and digests with PstI, EcoRI, and HindIII. It also shows a linear regression analysis with distance vs log10 base pairs with R2 = 0.9993, indicating the relationship is linear.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
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    32 views2 pages

    Lamda Bokum

    Uploaded by

    api-306533742
    This document contains data from restriction enzyme digests of lambda DNA. It lists the distance migrated and estimated base pairs for uncut lambda DNA and digests with PstI, EcoRI, and HindIII. It also shows a linear regression analysis with distance vs log10 base pairs with R2 = 0.9993, indicating the relationship is linear.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 2

    Base Pairs Distance (in mm) log10 Base Pairs

    23630
    7
    4.3734637216
    9416
    9
    3.9738664498
    6557
    10
    3.8167051837

    L = Uncut Lamba No
    Enzyme
    Distance
    (in mm)

    Estimated
    Base Pairs
    6 35900.459

    4.5
    4.4

    f(x) = - 0.1876165303

    4.3
    4.2
    4.1
    4
    3.9
    3.8
    3.7
    3.6
    3.5
    6.5

    7.5

    P = Pstl Restriction
    Digest of Lamba DNA
    Distance
    (in mm)
    8
    12

    E = EcoRi Restriction H = Hindlll Restriction


    Digest of Lamba DNA Digest of Lamba DNA

    Estimated Distance Estimated Distance


    Base Pairs
    (in mm) Base Pairs (in mm)
    15132.1278
    7 23307.7312
    7
    2688.43784
    10 6378.22742
    8
    11 4140.95013
    9

    Estimated
    Base Pairs
    23307.7312
    15132.1278
    9824.26345

    f(x) = - 0.1876165303x + 5.6806883806

    Column C
    Linear (Column C)

    7.5

    8.5

    9.5

    10

    10.5

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