Coagulation Testing

Partial Thromboplastin Time (PTT)

(Intrinsic Pathway)
Contact Activation

Factor XIIa + Calcium

Prothrombin Time (PT)
(Extrinsic Pathway)
Tissue Factor/Phospholipid+Calcium Factor XIa Factor XI

Factor VIIa Factor IXa + Factor VIIIa Factor IX

Factor X Factor Xa + Factor Va

Prothrombin Thrombin

Fibrinogen Fibrin

Key: solid arrows indicate activation of a coagulation factor by the factor above it in the cascade,
dotted arrows indicate activation of factor V and factor VIII by thrombin.

© 2008 American Association for Clinical Chemistry