715-542 Artificial Insemination

& Reproductive Technology

การผสมเทียม ในกระบือ

ผศ. สพ.ญ.ดร.สุณรี ัตน เอี่ยมละมัย

คณะสัตวแพทยศาสตร มหาวิทยาลัยขอนแกน
OESTROUS BUFF-COW WAS MATED
BY A BUFF-BULL
Swamp Buffalo
Milking Buffalo (murrah)
SEMEN COLLECTION AND A.I. IN BUFFALO

SEXUAL BEHAVIOUR (MATING BEHAVIOUR)

is under the influence of...

INTRINSIC FACTORS

- GENOTYPE (sexual weakness is hereditary factor)

=LESS SEX DRIVE THAN THE DAIRY BULLS

- ANDROGENS -fetal life-birth-puberty

PUBERTY 2.5-3 Yrs MATURITY 3-5 Yr

EXTRINSIC FACTORS

๘ NUTRITION,
๘ WORK LOAD,
๘ SEASON,
๘ LIGHT AND TEMPERATURE,
๘ SOCIAL INTERACTION -in group

FERTILITY, LIBIDO AND SEMEN QUALITY

DECLINE DURING HOT SUMMER -HARD WORK LOAD

 BUFFALO-BULL
AND ACTION TO OESTRUS BUFFALO-COW

• LICKING AND RUBBING OF THE FEMALE EXTERNAL

GENITALIA

• FLEHMEN RESPONSE

• REST HEAD AND CHIN ON THE FEMALE’S OR

TEASER’S RUMP

• TEMPERATURE AND TACTILE RECEPTORS ON

THE PENIS ARE IMPORTANT TO INTROMISSION

(A.V.)
 MATING BEHAVIOUR
• INTERESTING OF FEMALE (TEASER)

•ERECTION OF PENIS

•POSTURAL ADJUSTMENT

•MOUNTING -FORELEGS GRASP LUMBAR REGION

•SEARCHING AND INTROMISSION OF PENIS

•THRUST
•EJACULATION AND ORGASM

•DISMOUNT 1-2 mins

FALSE-MOUNT ON TEASER IN COLLECTION AREA
COLLECTOR WITH READY TO BE USED AV
 MOUNTING / INTROMISSION / THRUST /
EJACULATION = VERY FAST WITHIN 1-2 min ???
SEMEM EVALUATION

DO AS SOON AFTER
COLLECTION

APPLIED FROM BULL’S

SEMEN EVALUTION

MACRO /MICRO EXAMS

Volume = 2 ml

COLOR = MILKY WHITE

CONSISTENCY = CREAMY AND THICK

Ph = 6.9

INDIVIDUAL SPERM MOTILITY = 50-80%

CONCENTRATION = 800-1,000 mil/ml

VIABILITY = AT LEAST 75% - 85%

MORPHOLOGY = abnormal head < 5%

= abnormal midpiece and tails <10%
DUE TO SIMILARITY OF ANATOMY / PHYSIOLOGY
OF REPRODUCTIVE ORGANS
OF BOTH BUFFALOES AND CATTLE

SEMEN COLLECTION AND PROCESSING

ARTFICIAL INSEMINATION TECHNIQUE

ADOPTION WITH MODIFICATION

LIQUID SEMEN DEEP FROZEN SEMEN

DILUENT = EGG YOLK TRIS (YET) = CR 50%

SEMEN PROCESSING

AFTER PASS THE EVALUATION QUALITY

COOLING AND FREEZING

DILUTORS= EYT (egg yolk tris)

- COOLED TO 5 ‘c IN 1-1.5 hr

- PACKED IN 0.25 ml STRAW IN 5‘c 4-8 hr

- IN LIQ. NITROGEN VAPOUR -120’c FOR 10 min

- THEN DIP IN LIQ. NITROGEN -196’c

- QC
 715 633 Artificial Insemination

SEMEN COLLECTION
AND
ARTIFICIAL INSEMINATION

IN BUFFALO

BY
Suneerat Aiumlamai
Dept. of Surgery and Theriogenology
Fac. of Veterinary Medicine
Khon Kaen University
July 1999
IMPORTANT STEPS ON A.I. WORK

1. BULL SELECTION

2. TRAINING OF THE BULL AND OPRATOR

3. SEMEN COLLECTION / EVALUATION

4. MATING BEHAVIOUR

5. SEMEN PROCESSING / STORAGE

6. A.I. TECHNIQUE

7. RECORD / CONCEPTION RATE

 • ONCE A WEEK

• 2 COLLECTIONS
10 MIN. APART

•AV-TEMP = 42 ‘c
(air + lubricant)

• VOLUME 2 ml /
EJACULATATE

• 800-1,000 mil/ml

ARTIFICIAL VAGINA
FROZEN SEMEN THAWING PROCESS

WARM WATER 37’c-42’c FOR 15-20 sec

SEMEN THAWING AND AI-TECHNIQUE

SIMILAR AS IN COW
Fresh-Semen INSEMINATION
RECTAL PALPATION FEMALE BUFFALO
BUFFALO IN THAILAND IN 1985
What ever will be, will be …… in near future ???
NEXT GENERATION ????
WHY ???

•THAI- RURAL

•AGRICULTURE

•SUSTAINABLE

•PEOPLE

•LIVE / LIFE

•VETERINARIAN
BUFFALO BULL