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  • Grafico de Cinética Enzimática

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    Andreiita_23
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    This document contains a graph and data table summarizing kinetic enzyme assays performed with and without an inhibitor. The graph of 1/V versus 1/[S] shows non-competitive inhibition, with the x-intercept indicating an inhibitor constant (Ki) of 0.5. Kinetic parameters calculated from the graph include a Km of 2.77 mM and Vmax of 4.878 mM/min.

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    This document contains a graph and data table summarizing kinetic enzyme assays performed with and without an inhibitor. The graph of 1/V versus 1/[S] shows non-competitive inhibition, with the x-intercept indicating an inhibitor constant (Ki) of 0.5. Kinetic parameters calculated from the graph include a Km of 2.77 mM and Vmax of 4.878 mM/min.

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    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
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    46 views1 page

    Grafico de Cinética Enzimática

    Uploaded by

    Andreiita_23
    This document contains a graph and data table summarizing kinetic enzyme assays performed with and without an inhibitor. The graph of 1/V versus 1/[S] shows non-competitive inhibition, with the x-intercept indicating an inhibitor constant (Ki) of 0.5. Kinetic parameters calculated from the graph include a Km of 2.77 mM and Vmax of 4.878 mM/min.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
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    of 1

    Grafico de Cinética Enzimática

    [S] mM Vo (mM x min -1) Vo con I (mM x min -1)


    1 1,3 0,8
    2 2,0 1,2
    4 2,8 1,7
    8 3,6 2,2
    12 4,0 2,4

    1/[S] 1/Vo 1/Vo + I


    1 0,769 1,250
    0,5 0,500 0,833
    0,25 0,357 0,588
    0,125 0,277 0,454
    0,083 0,250 0,416

    Inhibición no competitiva

    1.5

    1.3

    1.1

    0.9

    1/V 0.7 α=1


    X
    0.5

    0.3

    0.1

    -0.6 -0.4 -0.2-0.1 0 0.2 0.4 0.6 0.8 1 1.2


    1/[s]

    Km = -(1/-0,36)
    Km = 2,77 mM

    Vmax = α = 1/0,205 = 4,878 (mM x min -1)

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