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  • Bioquimica - Graficas Michaelis-Menten

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    Alejandro Peñafiel
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    This document contains data from experiments measuring enzyme kinetics. It includes tables of initial rates of reaction (Vo) at different substrate (S) concentrations, as well as Lineweaver-Burk plots of the reciprocal of initial rate (1/Vo) versus the reciprocal of substrate concentration (1/S). The Lineweaver-Burk plots are used to determine the Michaelis-Menten constant (KM) and maximum reaction rate (Vmax) for the enzyme by calculating the x-intercept and slope.

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    Michaelis-Menten

    Original Title

    Bioquimica- Graficas Michaelis-Menten

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    This document contains data from experiments measuring enzyme kinetics. It includes tables of initial rates of reaction (Vo) at different substrate (S) concentrations, as well as Lineweaver-Burk plots of the reciprocal of initial rate (1/Vo) versus the reciprocal of substrate concentration (1/S). The Lineweaver-Burk plots are used to determine the Michaelis-Menten constant (KM) and maximum reaction rate (Vmax) for the enzyme by calculating the x-intercept and slope.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    15 views3 pages

    Bioquimica - Graficas Michaelis-Menten

    Uploaded by

    Alejandro Peñafiel
    This document contains data from experiments measuring enzyme kinetics. It includes tables of initial rates of reaction (Vo) at different substrate (S) concentrations, as well as Lineweaver-Burk plots of the reciprocal of initial rate (1/Vo) versus the reciprocal of substrate concentration (1/S). The Lineweaver-Burk plots are used to determine the Michaelis-Menten constant (KM) and maximum reaction rate (Vmax) for the enzyme by calculating the x-intercept and slope.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    of 3

    (mM/min) (mM/min) (mM/min) (min/mM) (min/mM) (min/mM)

    S(mM) Io (1,5Mm) (1,5Mm) 1/S(mM) 1/Io (1,5Mm) (1,5Mm)


    0,2 1,67 0,63 0,83 5 0,598802395 1,587301587 1,204819277
    0,4 2,86 1,18 1,43 2,5 0,34965035 0,847457627 0,699300699
    0,8 4,44 2,11 2,22 1,25 0,225225225 0,473933649 0,45045045
    1,6 6,15 3,48 3,08 0,625 0,162601626 0,287356322 0,324675325
    3,2 7,62 5,16 3,81 0,3125 0,131233596 0,19379845 0,262467192

    Lineweaver - Burk
    2

    1/IA
    1,5 y = 0,2973x + 0,102
    R = 1
    1/IB 1/Io
    1
    y = 0,201x + 0,1989 1/IA (1,5Mm)
    R = 1
    1/IB(1,5Mm)
    1/I

    0,5
    1/Io
    y = 0,0997x + 0,1003 Lineal (1/Io)
    0 R = 1
    Lineal (1/IA (1,5Mm))
    -4 -2 0 2 4 6
    Lineal (1/IB(1,5Mm))
    -0,5

    -1
    1/S
    Michaelis - S(mM) 0,1 0,2 0,5 1 2 3,5 5
    Menten Vo (mM/s) 0,04 0,08 0,17 0,24 0,32 0,39 0,42
    Linewearver- 1/S 10 5 2 1 0,5 0,28571429 0,2
    Burk 1/Vo 25 12,5 5,88235294 4,16666667 3,125 2,56410256 2,38095238

    Michaelis - Menten
    0,45
    0,4
    0,35
    0,3
    Vo (mM/s)

    0,25
    y = 0,1009l n(x) + 0,2523
    0,2
    R = 0,9927
    0,15
    0,1
    0,05
    0
    0 1 2 3 4 5 6
    S(mM)
    Lineweaver - Burk
    30

    25

    20
    1/Vo

    15

    10 y = 2,2865x + 1,7442
    R = 0,9977
    5

    0
    0 2 4 6 8 10 12
    1/S

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